GEO DataSets

(Submitter supplied) SAGE profile of rat hippocampus mRNA from Wistar rats

Organism:

Rattus norvegicus

Type:

Expression profiling by SAGE

Platform:

GPL23

1 Sample

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(Submitter supplied) Serial analysis of gene expression (SAGE) was used to get a global view of the gene profile in human hippocampus. A library were generated from control hippocampus, obtained by rapid autopsy. Keywords: hippocampus human inventory genes

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

1 Sample

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(Submitter supplied) Evaluation of extraocular muscle (EOM) critical period by expression profiling in dark reared rat. Data form part of publication: Investigative Ophthalmology and Visual Sciences 44: 3842-3855, 2003. Keywords: other

Organism:

Rattus norvegicus

Type:

Expression profiling by SAGE

Platform:

GPL23

2 Samples

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(Submitter supplied) SAGE profile of the rat superior olivary complex (SOC). Rats were 60 days old (P60). Keywords: other

Organism:

Rattus norvegicus

Type:

Expression profiling by SAGE

Platform:

GPL23

1 Sample

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(Submitter supplied) experimental group and control group in rat post-status epilepticus model. Keywords: parallel sample

Organism:

Rattus norvegicus

Type:

Expression profiling by SAGE

Platform:

GPL23

2 Samples

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(Submitter supplied) SAGE analysis of genes expressed in rat brain microvessels. Keywords: Novel gene identification

Organism:

Rattus norvegicus

Type:

Expression profiling by SAGE

Platform:

GPL23

1 Sample

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(Submitter supplied) Three groups of Wistar rats with different manipulated levels of corticosterone were used to identify corticosteroid-responsive genes in hippocampus: 1. rats were adrenalectomised (ADX) 2. In addition to ADX these rats were implanted with a subcutaneous pellet containing 20 mg corticosterone and 80% cholesterol (ADX + low CORT) 3. In addition to ADX and implantation of a corticosterone pellet, these rats received a subcutaneous injection with corticosterone (1 mg/kg bodyweight) three hours prior to decapitation ((ADX + low CORT) + acute high CORT). more...

Organism:

Rattus norvegicus

Type:

Expression profiling by SAGE

Platform:

GPL23

3 Samples

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(Submitter supplied) APOE4 allele is a major risk factor for late-onset Alzheimer disease (AD). The mechanism of action of APOE in AD remains unclear. To study the effects of APOE alleles on gene expression in AD, we have analyzed the gene transcription patterns of human hippocampus from APOE3/3, APOE3/4, APOE4/4 AD patients and normal control using Serial Analysis of Gene Expression (SAGE). Using SAGE, we found gene expression patterns in hippocampus of APOE3/4 and APOE4/4 AD patients differ substantially from those of APOE3/3 AD patients. more...

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platforms:

GPL4 GPL1485

6 Samples

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(Submitter supplied) This library was generated from normal human thyroid tissue collected at routine autopsy. Standard SAGE protocol was used (Velculescu et al. Science 1995). Data from this library was published in: 'Pauws E, Moreno JC, Tijssen M, Baas F, de Vijlder JJ, Ris-Stalpers C. J Clin Endocrinol Metab. 2000 May;85(5):1923-7' Data was generated and analyzed at the laboratory of Pediatric Endocrinology, Academic Medial Center, Amsterdam, The Netherlands.

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

1 Sample

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(Submitter supplied) SAGE libraries were prepared from mRNA of cultured bovine glomerular and aortic endothelial cells. Cells both cell lines were positive for acetylated LDL uptake and vWF expression, and were proven to be free of non-endothelial cells. Cultures were studied at passage 9-10. The cells were confluent, cultured in parallel, under identical conditions. Cells were plated on gelatin coated cell culture plastic in RPMI 1640 medium containing 10% FBS and streptomycin/penicillin. more...

Organism:

Bos taurus

Type:

Expression profiling by SAGE

Platform:

GPL223

2 Samples

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(Submitter supplied) SAGE analysis of fully grown ovarian follicles has been carried out to supply a resource for identification of important molecules involved in vertebrate oogenesis and in early stages of embryonic development. Keywords: SAGE

Organism:

Danio rerio

Type:

Expression profiling by SAGE

Platform:

GPL3209

1 Sample

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(Submitter supplied) Rice blast, caused by the fungal pathogen Magnaporthe grisea, is a devastating disease causing tremendous yield loss in rice production. The public availability of the complete genome sequence of M. grisea provides ample opportunities to understand the molecular mechanism of its pathogenesis on rice plants at the transcriptome level. To identify all the expressed genes encoded in the fungal genome, we have analyzed the mycelium and appressorium transcriptomes using MPSS, RL-SAGE and oligoarray methods. more...

Organism:

Pyricularia grisea

Type:

Expression profiling by array; Expression profiling by SAGE

Platforms:

GPL4080 GPL4085

2 Samples

Download data: TXT

(Submitter supplied) To identify functions that distinguish the posterior and median cells producing fibroin and sericin in the silk gland of Bombyx mori, serial analysis of gene expression (SAGE) profiles from both silk gland regions were analyzed and compared. The construction of a B. mori reference tag collection extracted from a set of 38000 Bombyx EST sequenced from the 3’ side, helped annotating the SAGE libraries. more...

Organism:

Bombyx mori

Type:

Expression profiling by SAGE

Platform:

GPL2095

2 Samples

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(Submitter supplied) The analysis of differentially expressed genes is a powerful approach to elucidate the genetic mechanisms underlying the morphological and evolutionary diversity among serially homologous structures, both within the same organism (e.g., hand vs. foot) and between different species (e.g., hand vs. wing). In the developing embryo, limb-specific expression of Pitx1, Tbx4, and Tbx5 regulates the determination of limb identity. more...

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platform:

GPL11

2 Samples

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(Submitter supplied) PURPOSE: To compare the gene expression profiles of normal human corneal endothelium with Fuchs' corneal endothelium, by using serial analysis of gene expression (SAGE). METHODS: Three pairs of normal human corneas were obtained from eye banks. Thirteen bisected Fuchs' corneal buttons were processed at the time of corneal transplantation. The endothelia of normal and Fuchs'-affected corneas were stripped, and total RNA was isolated. more...

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

2 Samples

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(Submitter supplied) Triticum aestivum cultivars Scorpion 25 and Xi 19 were grown under both normal and hot/dry conditions. We compared the effect of these two growth conditions on these two closely related varieties.

Organism:

Triticum aestivum

Type:

Expression profiling by SAGE

Platform:

GPL7079

6 Samples

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(Submitter supplied) Limbal and central regoins of the rat cornea epithelium were used to constract 2 SAGE libraries, in order to identify candidate genes to distinguish stem cell from differentiated central cornea epithelium cells. Keywords: gene expression SAGE-based, count

Organism:

Rattus norvegicus

Type:

Expression profiling by SAGE

Platform:

GPL23

2 Samples

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(Submitter supplied) Increasing evidence suggests that low-abundant transcripts may play fundamental roles in biological processes. In an attempt to estimate the prevalence of low-abundant transcripts in eukaryotic genomes, we performed a transcriptome analysis in Drosophila using the SAGE technique. We collected 244,313 SAGE tags from transcripts expressed in Drosophila embryonic, larval, pupae, adult, and testicular tissue. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by SAGE

Platform:

GPL1882

5 Samples

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(Submitter supplied) We performed a genome-wide analysis of gene expression in primary human CD151 myeloid progenitor cells. By using the serial analysis of gene expression (SAGE) technique, we obtained quantitative information for the expression of 37,519 unique SAGE-tag sequences. Of these unique tags, (i) 25% were detected at high and intermediate levels, whereas 75% were present as single copies, (ii) 53% of the tags matched known expressed sequences, 34% of which were matched to more than one known expressed sequence, and (iii) 47% of the tags had no matches and represent potentially novel genes. more...

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

1 Sample

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(Submitter supplied) PURPOSE: To provide a detailed gene expression profile of the normal postnatal mouse cornea. METHODS: Serial analysis of gene expression (SAGE) was performed on postnatal day (PN)9 and adult mouse (6 week) total corneas. The expression of selected genes was analyzed by in situ hybridization. RESULTS: A total of 64,272 PN9 and 62,206 adult tags were sequenced. Mouse corneal transcriptomes are composed of at least 19,544 and 18,509 unique mRNAs, respectively. more...

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platform:

GPL11

2 Samples

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