GEO DataSets

(Submitter supplied) Acute quadriplegic myopathy (AQM) or critical illness myopathy (CIM) is frequently observed in intensive care unit (ICU) patients. In order to elucidate duration-dependent effects of the ICU intervention on molecular and functional networks that control the muscle wasting and weakness in AQM, gene expression profile was analyzed at time points varying from 6 hours to 14 days in a unique experimental rat model mimicking ICU conditions, i.e., post-synaptically paralyzed, mechanically ventilated and extensively monitored animals.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL6247

28 Samples

Download data: CEL, TXT

(Submitter supplied) AQM shows acute muscle wasting and weakness. Key aspects of AQM include muscle atrophy and myofilament loss. Gene expression profiling, using muscle biopsies from AQM, neurogenic atrophy and normal controls, showed that both myogenic and neurogenic atrophy share induction of myofiber-specific ubiquitin/proteosome pathways while only the AQM shows a specific strong induction of transforming growth factor (TGF)-beta/MAPK pathways. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL96 GPL8300

10 Samples

Download data: CEL

(Submitter supplied) Background: The aim of this study is to improve our understanding of the mechanisms underlying the sparing of masticatory muscles relative to limb muscles in ICU patients with acute quadriplegic myopathy (AQM) by using a unique porcine ICU model, i.e., 5-day longitudinal experiments where animals are sedated, mechanically ventilated and exposed to factors triggering AQM, such as muscle unloading, endotoxin-induced sepsis, and systemic exposure to CS and NMBA. more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL3533

18 Samples

Download data: CEL, TXT

(Submitter supplied) Critically ill intensive care unit (ICU) patients commonly develop severe muscle wasting and impaired muscle function, leading to delayed recovery, with subsequent increased morbidity and financial costs, and decrease quality of life of survivors. Acute Quadriplegic Myopathy (AQM) is one of the most common neuromuscular disorders associated with ICU-acquired muscle weakness. Although there are no available treatments for the ICU-acquired muscle weakness, it has been demonstrated that early mobilization can improve its prognosis and functional outcomes. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS4563

Platform:

GPL6247

46 Samples

Download data: CEL

Temporal analysis of loaded leg gastrocnemius of females (subjected to neuromuscular blockade, mechanical ventilation) following unilateral passive mechanical loading (PML). Results provide insight into molecular basis of the beneficial effects of PML on muscle mass and function in this ICU model.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, transformed count, 2 protocol, 3 time sets

Platform:

GPL6247

Series:

GSE37944

46 Samples

Download data: CEL

(Submitter supplied) The goal of this study was to identify changes in muscle gene expression that may contribute to loss of adaptability of old muscle. Muscle atrophy was induced in young adult (6-month) and old (32-month) male Brown Norway/F344 rats by two weeks of hind limb suspension (HS) and soleus muscles were analyzed by cDNA microarrays. We conclude that a cold shock response may be part of a compensatory mechanism in muscles undergoing atrophy to preserve remaining muscle mass and that RBM3 may be a therapeutic target to prevent muscle loss.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL7045

18 Samples

Download data: CSV

(Submitter supplied) To identify atrophy genes directly targeted by Bcl-3 transactivator at a genome wide level, we performed whole transcript expression array and ChIP-seq for muscles from weight bearing or 5-day hind limb unloaded mice. Genes that showed increased expression with unloading and a Bcl-3 peak in the promoter (from ChIP-seq data) were considered as Bcl-3 direct targets during disuse atrophy. Using ChIP array, we identified 241 direct targets for Bcl-3. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11078

8 Samples

Download data: CEL

(Submitter supplied) Background : The aim of this study is to improve our understanding of the mechanisms underlying the role of sepsis in the limb muscles of ICU patients with acute quadriplegic myopathy (AQM) by using a unique porcine ICU model, i.e., 5-day longitudinal experiments where animals are sedated, mechanically ventilated and exposed to factor triggering AQM that is endotoxin-induced sepsis. Results : An increased expression of genes involved in chemokine activity and transcriptional regulation. more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL3533

8 Samples

Download data: CEL

(Submitter supplied) Following spinal cord injury, skeletal muscle loss is rapid. This severe atrophy is attributed to declines in protein synthesis and increases in protein breakdown. However, the signaling mechanisms controlling these changes are not well understood. Nine male patients and one female patient with spinal cord injury (SCI) (Mean ± SEM = 43.9 ± 6.7 yrs) were recruited for this study. Six patients were quadriplegics and four patients were paraplegics. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

20 Samples

Download data: CEL, CHP

(Submitter supplied) Although short-term disuse does not result in measurable muscle atrophy, studies suggest that molecular changes associated with protein degradation may be initiated within days of the onset of a disuse stimulus. We examined the global gene expression patterns in sedentary men (n = 7, mean age ± S.D = 22.1 ± 3.7 yr) following 48h unloading (UL) via unilateral lower limb suspension and 24h reloading (RL). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3762

Platform:

GPL570

21 Samples

Download data: CEL, CHP

Analysis of left vastus lateralis muscle biopsies from 7 sedentary men following unloading (48 hr) via unilateral lower limb suspensionand and subsequent reloading (24 hr). Results provide insight into molecular events underlying the earliest stages of skeletal muscle disuse and reambulation.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 7 individual, 3 protocol sets

Platform:

GPL570

Series:

GSE21496

21 Samples

Download data: CEL, CHP

(Submitter supplied) We showed that nandrolone attenuated subacute, but not acute, denervation atrophy and upregulation of MAFbx. The present study explored the molecular determinants for this time-dependent effect using microarray analysis to identify genes that were differentially regulated by administration of nandrolone for 7 days beginning either concomitantly with denervation (7 days) or 29 days later (35 days)

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

12 Samples

Download data: CEL, TXT

(Submitter supplied) Myostatin is a highly conserved Transforming Growth Factor beta family member which negatively regulates muscle development. Double-muscled (DM) cattle have a loss-of-function mutation in their myostatin gene responsible for a hypermuscular phenotype mainly due to hyperplasia. Thus these animals represent a good model for understanding the mechanisms at the origin of muscular hypertrophy. In order to identify individual genes or networks that may be myostatin targets, we looked for genes differentially expressed between DM and normal (NM) animals (n=3 per group) in the semitendinosus muscle (a hypertrophied muscle in DM animals) at 260 days of fetal development (intensive muscle biochemical differentiation). more...

Organism:

Bos taurus; Mus musculus

Type:

Expression profiling by array

Platform:

GPL4049

24 Samples

Download data

(Submitter supplied) Background: Skeletal muscle wasting and impaired muscle function in response to mechanical ventilation and immobilization in intensive care unit (ICU) patients are clinically challenging partly due to (i) the poorly understood intricate cellular and molecular networks; and (ii) the unavailability of an animal model mimicking this condition. By employing a unique porcine model mimicking the conditions in the ICU with long-term mechanical ventilation and immobilization, we have analyzed the expression profile of skeletal muscle biopsies taken at three time points during a five-day period. more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL3533

24 Samples

Download data: CEL, CHP