GEO DataSets

(Submitter supplied) Study of brain regions from GRN KO, Heterozygous and WT mice at different time points (2-6-9 months)

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5501

Platform:

GPL6885

99 Samples

Download data: TXT

(Submitter supplied) Progranulin (GRN) mutations cause frontotemporal dementia (FTD), but GRN's function in the CNS remains largely unknown. To identify the pathways downstream of GRN, we used weighted genome co-expression network analysis (WGCNA) to develop a systems-level view of transcriptional alterations in a human neural progenitor model of GRN-deficiency. This highlighted key pathways such as apoptosis and ubiquitination in GRN deficient human neurons, while revealing an unexpected major role for the Wnt signaling pathway, which was confirmed by analysis of gene expression data from postmortem FTD brain. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6883

12 Samples

Download data: TXT

Analysis of brain cortices, cerebella, and hippocampi of progranulin (GRN) deficient mutants at ages 2, 6, and 9 months. GRN mutations cause frontotemporal dementia. Results provide insight into the function of GRN in the central nervous system.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 3 age, 2 gender, 3 genotype/variation, 3 tissue sets

Platform:

GPL6885

Series:

GSE31840

99 Samples

Download data

(Submitter supplied) To understand how haploinsufficiency of progranulin (PGRN) protein causes frontotemporal dementia (FTD), we created induced pluripotent stem cells (iPSC) from patients carrying the GRNIVS1+5G>C mutation (FTD-iPSCs). FTD-iPSCs were fated to cortical neurons, the cells most affected in FTD and known to express PGRN. Although generation of neuroprogenitors was unaffected, their further differentiation into neurons, especially CTIP2-, FOXP2- or TBR1-TUJ1 double positive cortical neurons, was significantly decreased in FTD-neural progeny. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

6 Samples

Download data: XLSX

(Submitter supplied) FTLD-U is the most common pathological correlate of the neurodegenerative dementia frontotemporal dementia We used microarrays to perform global expression profiling of FTLD-U brain samples Keywords: disease

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3459

Platform:

GPL571

56 Samples

Download data: CEL

Analysis of various brain regions of patients who suffered from frontotemporal lobar degeneration with ubiquitinated inclusions (FTLD-U). Presence of progranulin (GRN) mutations determined. GRN mutations are associated with FTLD-U. Results identify expression signatures for GRN subtypes of FTLD-U.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 3 disease state, 3 tissue sets

Platform:

GPL571

Series:

GSE13162

56 Samples

Download data: CEL

(Submitter supplied) Fz2Fz7 double knock out mouse microarray (E8.5)

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL, CHP

(Submitter supplied) Multiple FTD patient-specific iPSC lines were generated for the first time, Human neurons of progranulin haploinsufficiency have been established. PGRN S116X neurons are more sensitive to kinase inhibitors-induced cell stress, which can be rescued by ectopic progranulin expression, revealing progranulin-dependent cellular defects in FTD. Microarray analysis reveals that the serine/threonine kinase S6K2 (RPS6KB2) and other genes involved in MAPK signaling are dysregulated specifically in neurons with progranulin deficiency.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

32 Samples

Download data: TXT

(Submitter supplied) Patients with frontotemporal dementia (FTD) resulting from granulin (GRN) haploinsufficiency have reduced levels of progranulin and exhibit dysregulation in inflammatory and lysosomal networks. Microglia produce high levels of progranulin, and reduction of progranulin in microglia alone is sufficient to recapitulate inflammation, lysosomal dysfunction, and hyperproliferation in a cell-autonomous manner. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

13 Samples

Download data: TXT

(Submitter supplied) RNA was extracted from cortex of 1 year old wildtype, heterozygous or homozygous PGRN knockout mice. The "SAMPLE_ID" sample characteristic is a sample identifier internal to Genentech. The ID of this project in Genentech's ExpressionPlot database is PRJ0007808

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

15 Samples

Download data: TSV

(Submitter supplied) 1 year old mice were perfused and brains were dissociated. Cells were fixed, immunolabeled and FACS sorted. RNA was extracted from neuron, astrocyte, and microglial cell populations. Typical RIN=4-5 for neurons, 6-8 for astrocytes, and 5-7 for microglia. Typical RNA yields ~100ng for neurons, ~20ng for microglia, and ~10ng for astrocytes. cDNA was generated from up to 25 ng of total RNA using Nugen’s RNA-Seq method for low-input RNA samples, Ovation RNA-Seq System V2 (NuGEN). more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

29 Samples

Download data: TSV

(Submitter supplied) Nails protect the soft tissue of the tips of digits in humans. In birds and mammals the equivalent claws are used for purposes including capturing prey, digging, climbing, fighting and maintaining dexterity and balance. The molecular mechanism of nail (and claw) development is largely unknown, but we have recently identified a Wnt receptor gene, Frizzled6 (Fzd6), as mutated in a human autosomal-recessive nail dysplasia. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6867

18 Samples

Download data: TXT