GEO DataSets

(Submitter supplied) To assess changes in the miRNA-ome during the early stages of endothelial differentiation, the expression of mature human miRNAs at days 2, 4 and 10 of endothelial differentiation (vs pluripotent time-matched samples) in SA461 hESC cell line was analysed in a two-channel microarray. Expression of the pluripotency-associated miRNAs miR-302a – d and miR-372 and miR-373 was significantly suppressed with progression of differentiation. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL14799

28 Samples

Download data: TXT

(Submitter supplied) Differentiation of human embryonic stem cells into endothelial cells (hESC-ECs) has the potential to provide an unlimited source of cells for novel transplantation therapies of ischemic diseases by supporting angiogenesis and vasculogenesis. In this study, we developed an extracellular matrix culture system for increasing endothelial differentiation and free from contaminating animal cells. We investigated the transcriptional changes that occur during endothelial differentiation of hESCs using whole genome microarray, and compared to human umbilical vein endothelial cells (HUVECs). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

16 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array; Expression profiling by array

Platforms:

GPL8733 GPL7363

29 Samples

Download data: CEL

(Submitter supplied) Pluripotent hESCs can differentiate into the three primary embryonic lineages (endoderm, mesoderm, ectoderm) as well as extraembryonic tissues. Definitive endoderm (DE) is the first step into the pathway to endoderm dreived tissues (pancreas, liver, gut, lung). We used microarrays to detail the changes in microRNA expression during the transition from pluripotent hESCs into definitive endoderm.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL8733

6 Samples

Download data: CEL

(Submitter supplied) Pluripotent hESCs can differentiate into the three primary embryonic lineages (endoderm, mesoderm, ectoderm) as well as extraembryonic tissues. Definitive endoderm is the first step into the pathway to endoderm dreived tissues (pancreas, liver, gut, lung) We used microarrays to detail the changes in microRNA expression during the transition from pluripotent hESCs into definitive endoderm

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL8733

6 Samples

Download data: CEL

(Submitter supplied) hESCs can differentiate into the three primary embryonic lineages (endoderm, mesoderm, ectoderm) as well as extraembryonic tissues. Definitive endoderm (DE) is the first step into the pathway to endoderm derived tissues: pancreas, liver, gut, lung. We used microarrays to detail the changes in mRNA expression during the transition from pluripotent hESCs into definitive endoderm.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL7363

9 Samples

Download data: TXT

(Submitter supplied) Pluripotent hESCs can differentiate into the three primary embryonic lineages (endoderm, mesoderm, ectoderm) as well as extraembryonic tissues. Definitive endoderm is the first step into the pathway to endoderm dreived tissues (pancreas, liver, gut, lung). We used microarrays to detail the changes in microRNA expression during the transition from pluripotent hESCs into definitive endoderm.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL8733

8 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Rattus norvegicus; Homo sapiens; Macacine gammaherpesvirus 4; Betapolyomavirus macacae; Mus musculus; Human gammaherpesvirus 8

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platforms:

GPL7089 GPL7088

38 Samples

Download data: GPR

(Submitter supplied) Human embryonic stem (hES) cells have unique features: self-renewal ability and pluripotency. They can be continuously cultured in undifferentiated state and give rise to cells and tissues of all three germ layers. Thus hES cells provide a resource not only for cell replacement therapy but also for studying human developmental biology. We aimed to identify the unique signature of miRNAs in human embryonic stem cells. more...

Organism:

Mus musculus; Human gammaherpesvirus 8; Betapolyomavirus macacae; Rattus norvegicus; Macacine gammaherpesvirus 4; Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL7089

21 Samples

Download data: GPR

(Submitter supplied) Human embryonic stem (hES) cells have unique features: self-renewal ability and pluripotency. They can be continuously cultured in undifferentiated state and give rise to cells and tissues of all three germ layers. Thus hES cells provide a resource not only for cell replacement therapy but also for studying human developmental biology. However, much work needs to be done to understand the molecular mechanisms responsible for the maintenance of the undifferentiatiation state and the differentiation process of human embryonic stem cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL7088

17 Samples

Download data: GPR

(Submitter supplied) We used massively parallel pyrosequencing to discover and characterize microRNAs (miRNAs) expressed in human embryonic stem cells (hESCs). Sequencing of small RNA cDNA libraries derived from undifferentiated hESC and from isogenic differentiating cultures yielded a total of 425,505 high-quality sequence reads. A custom data analysis pipeline delineated expression profiles for 191 previously annotated miRNAs, 36 novel miRNAs highly conserved across vertebrates, and 291 novel candidate miRNAs. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL10400

2 Samples

Download data: FA, FNA

(Submitter supplied) We aimed to determine whether overexpression of endoderm-specific miRNA may affect hESC differentiation. To this end, we analyzed the effect of lentiviral-based overexpression of liver-specific miR-122 on hESC differentiation, using genomewide gene microarrays. Stable overexpression of endoderm-specific miR-122 in hESC resulted in increased expression of a few endodermal markers in spontaneously-differentiating hESC, but had no clear effect on directing differentiation towards an endodermal fate; rather, it delayed the general differentiation of hESC. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3470

Platform:

GPL571

5 Samples

Download data: CEL

Analysis of embryonic stem cells (ESCs) overexpressing wild-type miR-122. miR-122 is an endoderm specific microRNA. Results provide insight into the role of miR-122 in the differentiation of ESCs.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent sets

Platform:

GPL571

Series:

GSE13460

5 Samples

Download data: CEL

(Submitter supplied) This experiment explored what miRNA differences and similarities exist between endothelial cells obtained from different vascular beds.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL10850

16 Samples

Download data: TXT

(Submitter supplied) Human ES or iPS Cells were differentiated into endothelial cells (ECs) defined by expression of CD31 (PECAM1) and CD144 (VE-Cadherin) on the cell surface. All ES or iPS derived ECs were greater than 90% double positive for these two markers. These in vitro derived ECs were compared to each other and to ECs from primary cell sources; arterial (aortic Ecs), venous (saphenous vein) and lymphatic.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

12 Samples

Download data: CEL

(Submitter supplied) Increased monocyte adhesion to dysfunctional endothelial cells (ECs) orchestrated by chemokines plays an important role in arterial inflammation during atherosclerosis. Endothelial microRNAs (miRNAs) processed by the RNase Dicer1 determine the phenotype of ECs by posttranscriptional regulation of gene expression. However, the impact of endothelial miRNAs on endothelial inflammation and atherosclerosis is currently unclear. more...

Organism:

Mus musculus

Type:

Expression profiling by RT-PCR

Platform:

GPL18082

6 Samples

Download data: TXT

(Submitter supplied) Increased monocyte adhesion to dysfunctional endothelial cells (ECs) orchestrated by chemokines plays an important role in arterial inflammation during atherosclerosis. Endothelial microRNAs (miRNAs) processed by the RNase Dicer1 determine the phenotype of ECs by posttranscriptional regulation of gene expression. However, the impact of endothelial miRNAs on endothelial inflammation and atherosclerosis is currently unclear. more...

Organism:

Mus musculus

Type:

Expression profiling by RT-PCR

Platform:

GPL18082

6 Samples

Download data: TXT

(Submitter supplied) Mouse embryonic stem cells can spontaneously differentiate and assemble into a spherical embryoid body (EB) during suspension culture. The initial study aims to identify the up-regulated or down-regulated microRNAs during the differentiation process of pluripotent stem cells. From the microRNA profiling, we will focus on the microRNAs associated with mesodermal and endothelial differentiation of embryonic or induced pluripotent stem cells.

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array

Platform:

GPL21312

2 Samples

Download data: TXT

(Submitter supplied) Human embryonic stem (hES) cells have the capacities to propagate for extended periods and to differentiate into cell types from all three germ layers both in vitro and in vivo. These characteristics of self-renewal and pluripotency enable hES cells having the potential to provide an unlimited supply of different cell types for tissue replacement, drug screening, and functional genomics studies. The hES-T3 cells with normal female karyotype cultured on either mouse embryonic fibroblasts (MEF) in hES medium (containing 4 ng/ml bFGF) (T3MF) or feeder-free Matrigel in MEF-conditioned medium (supplemented with additional 4 ng/ml bFGF) (T3CM) were found to express very similar profiles of mRNAs and microRNAs, indicating that the unlimited self-renewal and pluripotency of hES cells can be maintained by continuing culture on these two conditions. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

6 Samples

Download data: CEL

(Submitter supplied) miRNA plays a critical role in a wide variety of biological processes Profiling miRNA expression during the differentiation of embryonic stem cells will help us to understand the regulation pathway of differentiation, therefore to explain disease mechanisms and to find possible therapeutical targets. In this study miRNA expressions were profiled during cardiomyocyte-specific differentiation of murine embryonic stem cells with high-throughput microarray platforms. more...

Organism:

synthetic construct; Mus musculus

Type:

Non-coding RNA profiling by array

Platforms:

GPL8786 GPL10849

32 Samples

Download data: CEL, TXT