GEO DataSets

(Submitter supplied) Brucella melitensis and Brucella canis differ by ~75 genes yet B. melitensis is highly virulent for humans while B. canis is considered rarely pathogenic. No identified bacterial factors or mechanisms account for this difference in virulence. To identify functional differences of these two bacteria, gene transcription was examined during infection of murine macrophages and compared to bacteria grown in broth. more...

Organism:

Brucella canis; Brucella ovis

Type:

Expression profiling by array

Platform:

GPL15024

4 Samples

Download data: CALLS, PAIR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Brucella canis; Brucella melitensis bv. 1 str. 16M; Brucella melitensis; Brucella ovis

Type:

Expression profiling by array

Platforms:

GPL15024 GPL15023

16 Samples

Download data: CALLS, PAIR

(Submitter supplied) Brucella melitensis and Brucella canis differ by ~75 genes yet B. melitensis is highly virulent for humans while B. canis is considered rarely pathogenic. No identified bacterial factors or mechanisms account for this difference in virulence. To identify functional differences of these two bacteria, gene transcription was examined during infection of murine macrophages and compared to bacteria grown in broth. more...

Organism:

Brucella ovis; Brucella canis

Type:

Expression profiling by array

Platform:

GPL15024

4 Samples

Download data: CALLS, PAIR

(Submitter supplied) Brucella melitensis and Brucella canis differ by ~75 genes yet B. melitensis is highly virulent for humans while B. canis is considered rarely pathogenic. No identified bacterial factors or mechanisms account for this difference in virulence. To identify functional differences of these two bacteria, gene transcription was examined during infection of murine macrophages and compared to bacteria grown in broth. more...

Organism:

Brucella ovis; Brucella canis

Type:

Expression profiling by array

Platform:

GPL15024

4 Samples

Download data: CALLS, PAIR

(Submitter supplied) Brucella melitensis and Brucella canis differ by ~75 genes yet B. melitensis is highly virulent for humans while B. canis is considered rarely pathogenic. No identified bacterial factors or mechanisms account for this difference in virulence. To identify functional differences of these two bacteria, gene transcription was examined during infection of murine macrophages and compared to bacteria grown in broth. more...

Organism:

Brucella melitensis; Brucella melitensis bv. 1 str. 16M

Type:

Expression profiling by array

Platform:

GPL15023

4 Samples

Download data: CALLS, PAIR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platforms:

GPL81 GPL32

16 Samples

Download data: CEL, CHP

(Submitter supplied) Brucella dynamically engage macrophages while trafficking to an intracellular replicative niche as macrophages, the first line of innate host defense, attempt to eliminate organisms. Brucella melitensis, B. neotomae, and B. ovis are highly homologous, yet exhibit a range of host pathogenicity and specificity. RAW 264.7 macrophages infected with B. melitensis, and B. ovis exhibit divergent patterns of bacterial persistence and clearance; conversely, B. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL81

11 Samples

Download data: CEL, CHP

(Submitter supplied) Hfq is a bacterial Sm-like protein which serves as a post-transcriptional regulator of global gene expression. It has been shown to be involved in a wide range of cellular processes and pathways. To characterize the regulation roles of Hfq in Brucella melitensis, the in vitro hfq induction conditions simulating intracellular environments were firstly defined, and then, the transcriptome of the hfq mutant was compared to that of the wild type strain. more...

Organism:

Brucella melitensis bv. 1 str. 16M; Brucella melitensis

Type:

Expression profiling by array

Platform:

GPL11117

4 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Brucella melitensis bv. 1 str. 16M; Brucella melitensis

Type:

Expression profiling by array

Platform:

GPL7612

30 Samples

Download data: GPR

(Submitter supplied) We sequenced the mRNA of Brucella melitensis 16M and otpR mutant under acid stress to identify genes that were regulated by regulator OtpR.

Organism:

Brucella melitensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17325

2 Samples

Download data: TXT, XLS

(Submitter supplied) The four-carbon sugar erythritol is an important component of B. melitensis pathogenesis. To determine the transcriptional response to erythritol, B. melitensis strain 16M was grown in the presence of either glucose or erythritol as a sole carbon source.

Organism:

Brucella melitensis bv. 1 str. 16M

Type:

Expression profiling by array

Platform:

GPL15533

4 Samples

Download data: CALLS, PAIR

(Submitter supplied) In this study, we employed the Illumina Genome Analyzer platform to perform a Digital Gene Expression (DGE) analysis of the peritoneal macrophages genome-wide transcriptome response to B.melitensis infection. Common changes in gene expression were observed among Brucella app. infected macrophages suggesting similar strategies were employed for their survival and replication, inducing anti-inflammatory and anti-apoptotic. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL9250

3 Samples

Download data

(Submitter supplied) A mini-microarray containing spotted oligos corresponding to 289 B. melitensis genes was used to identify candidate genes whose transcription is influenced by BlxR. This microarray contains genes encoding the type IV secretion system, flagella, transcriptional regulators, transporters, and proteins involved in outer membrane biogenesis, iron acquisition, and denitrification. RNA was prepared from both B. more...

Organism:

Brucella melitensis

Type:

Expression profiling by array

Platform:

GPL6170

14 Samples

Download data

(Submitter supplied) In this study we report that B. melitensis at the late logarithmic phase of growth are more invasive for HeLa cells than at mid logarithmic or stationary growth phases. Microarray analysis of B. melitensis gene expression identified 414 up- and 40 down-regulated genes in late-log growth phase compared to the stationary growth phase. The vast majority of the up-regulated genes in late-log cultures were those associated with DNA replication, transcription and translation, intermediate metabolism, energy production and conversion, membrane transport and cell envelope, biogenesis and outer membrane, while the down-regulated genes were distributed among several functional categories. more...

Organism:

Brucella melitensis

Type:

Expression profiling by array

Platform:

GPL6748

8 Samples

Download data: GPR

(Submitter supplied) Facultative intracellular Brucella infect and survive inside macrophages, and the outcome of macrophage-Brucella interaction is a basis for establishment of a chronic Brucella infection. The majority of Brucella are killed at the early infection stage. A subpopulation of virulent Brucella strains is instead trafficked to an intracellular replicative phagosome, and are resistant to further attack and begin to multiply dramatically. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

12 Samples

Download data: CEL

(Submitter supplied) Microarray analysis determined that 7.82% (244/3334) of Brucella abortus genes were up-regulated and 5.4% (180/3334) were down-regulated in RAW264.7 macrophages, compared to free-living bacteria in TSB

Organism:

Brucella abortus bv. 1 str. 9-941; Brucella abortus str. 2308 A

Type:

Expression profiling by array

Platform:

GPL17087

6 Samples

Download data: TXT

(Submitter supplied) Many pathogenic bacteria use a regulatory process termed Quorum Sensing (QS) to produce and detect small diffusible molecules to synchronize gene expression within a population. In Gram-negative bacteria, the detection and response to these molecules depend on transcriptional regulators belonging to the LuxR family. Such a system have been discovered in the intracellular pathogen Brucella melitensis, a Gram-negative bacteria responsible for brucellosis, a word-wide zoonosis remaining a serious public health concern in endemic countries. more...

Organism:

Brucella melitensis bv. 1 str. 16M

Type:

Expression profiling by array

Platform:

GPL5730

3 Samples

Download data: FTR

(Submitter supplied) The design (.ndf) file and description (.ngd) file are available as Supplementary files (at the foot of this record). Protocol: Microarray design and experimentation were made by NimbleGen Systems, INC from catalogue design for B. melitensis 16M chromosomes I (NC_003317) and II (NC_003318) with 20 probes per gene (10 perfect matches and 10 mismatches). Each probe (24 mer) was replicated three times on chip (design includes random GC probes). more...

Organism:

Brucella melitensis bv. 1 str. 16M

2 Series

9 Samples

Download data: NDF, NGD

(Submitter supplied) Investigation of whole genome gene expression level changes in a Brucella melitensis delta prlr mutant compared to the wild type strain. The mutants analyzed in this study are further described in A. Mirabella, R-M Yanez, R.M. Delrue, S. Uzureau, M.S. Zygmunt, A. Cloeckaert, X. De Bolle, J.J. Letesson (2012). The two component system PrlS/PrlR of Brucella melitensis is required for persistence in mice and appears to respond to ionic strength. more...

Organism:

Brucella melitensis bv. 1 str. 16M

Type:

Expression profiling by array

Platform:

GPL5730

6 Samples

Download data: PAIR

(Submitter supplied) Brucella spp. is an intracellular pathogen in vivo. The intracellular B. melitensis transcriptome was determined by initially enriched and then amplified B. melitensis RNA from total RNA of B. melitensis-infected HeLa cells. Analysis of microarray results identified 161 and 115 genes differentially expressed at 4 and 12 h p.i., respectively. Most of the genes (78%) differentially expressed were down-regulated at the earliest time point, but up-regulated (75%) at 12 h p.i. more...

Organism:

Brucella melitensis bv. 1 str. 16M; Brucella melitensis

Type:

Expression profiling by array

Platform:

GPL8156

18 Samples

Download data: GPR