GEO DataSets

(Submitter supplied) To test whether NDGA attenuate dyslipidemia and hepatic steatosis by enhancing fatty acid oxidation through activation of PPAR-α. Using wild type (WT, C57BL/6) fed with chow diet as control, WT mice were either fed with high-fat diet or high-fat diet with NDGA (2.5g/kg food); ob/ob mice were fed with either chow or chow with NDGA (2.5 g/kg food), and maintained on the respective diets for 16 weeks. The expression of lipid metabolism related genes in the liver of these mice were analyzed using Phalanx GPL6845 platform (Mouse OneArray V1). Together with other biochemical/physiological data, our results suggest that the beneficial actions of NDGA on dyslipidemia and hepatic steatosis in ob/ob mice are exerted primarily through enhanced fatty acid oxidation and energy utilization via the activation of PPAR- α receptor activity.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6845

5 Samples

Download data: GPR

(Submitter supplied) A high-fructose diet (HFrD) fed rat model of hypertriglyceridemia, dyslipidemia, insulin resistance and hepatic steatosis was employed to investigate the global transcriptional changes in the lipid metabolizing pathways in three insulin sensitive tissues:, liver, skeletal muscle and adipose tissue in response to chronic dietary administration of NDGA.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL13694

54 Samples

Download data: GPR, XLS

(Submitter supplied) Peroxisome proliferator-activated receptor alpha (PPARα) is a key regulator of hepatic fat oxidation that serves as an energy source during starvation. Vanin-1 has been described as a putative PPARα target gene in liver, but its function in hepatic lipid metabolism is unknown. We investigated the regulation of vanin-1, and total vanin activity, by PPARα in mice and humans. Furthermore, the function of vanin-1 in the development of hepatic steatosis in response to starvation was examined in Vnn1 deficient mice, and in rats treated with an inhibitor of vanin activity. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4872

Platform:

GPL11533

16 Samples

Download data: CEL

Analysis of liver from vanin-1 KOs fasted for 24 hrs. Vanin-1 is highly expressed in liver compared to other tissues. In the fasted state, liver switches to fatty acid oxidation and glucose production. Results provide insight into the role of vanin-1 in hepatic lipid metabolism during starvation.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 genotype/variation, 2 protocol sets

Platform:

GPL11533

Series:

GSE51712

16 Samples

Download data: CEL

(Submitter supplied) If the function of the nuclear receptor PPARa is well-known during a prolongated fasting, its hepatic biological function during feeding and refeeding conditions still needs to be investigated. Moreover, in vivo data collected so far on PPARa function during fasting were obtained using the total Ppara KO transgenic mouse model. To identify genes whose expression is under the strict dependence of hepatic PPARa activity, we generated a new mouse strain of PPARa-specific deletion in hepatocyte (albumin-Cre+/- Pparaflox/flox or LKO) and we compared them to total Ppara KO (KO), wild-type (WT) and liver WT (albumin-Cre-/- Pparaflox/flox or LWT) mice under three nutritional challenges. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10787

52 Samples

Download data: TXT

(Submitter supplied) Fenofibrate is a specific agonist of the nuclear receptor PPARa. To identify the gene expression under the strict dependence of hepatic PPARa activity, we generated a new mouse strain of PPARa-specific deletion in hepatocyte (albumin-Cre+/- Pparaflox/flox or LKO) and we compared them to total Ppara KO (KO), wild-type (WT) and liver WT (albumin-Cre-/- Pparaflox/flox or LWT) mice. We used microarrays to detail the global programme of gene expression in liver of Ppara LKO, LWT, Ppara KO and WT male mice.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10787

34 Samples

Download data: TXT

(Submitter supplied) *Objectives:* In addition to their well-known role in the control of cellular proliferation and cancer, cell cycle regulators are increasingly identified as important metabolic modulators. Genome wide association studies identified SNPs near the cell cycle regulator CDKN2A/p16INK4a (p16) as strongly associated with risk of developing type 2 diabetes (T2D). T2D is associated with numerous perturbations of hepatic lipid and glucose metabolism. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

8 Samples

Download data: CEL

(Submitter supplied) Sepsis induces a metabolic shift from glucose to fatty acid utilization that is key to survival for the organism. Here we used microarray analysis to characterize hepatic gene expression in conditions mimicking sepsis.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

24 Samples

Download data: CEL

(Submitter supplied) Hepatic metabolic derangements are key components in the development of fatty liver, insulin resistance, and atherosclerosis. SIRT1, a NAD+-dependent protein deacetylase, is an important regulator of energy homeostasis in response to nutrient availability. Here we demonstrate that hepatic SIRT1 regulates fatty acid metabolism by positively regulating PPAR-alpha. Hepatocyte-specific deletion of SIRT1 impairs PPAR-alpha signaling and decreased fatty acid beta-oxidation in the liver. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3666

Platform:

GPL7202

6 Samples

Download data: TIFF, TXT

Analysis of liver deficient for SIRT1. SIRT1, a NAD+-dependent protein deacetylase, is an important regulator of energy homeostasis in response to nutrient availability. Results provide insight into the role of hepatic SIRT1 in fatty acid metabolsim.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation sets

Platform:

GPL7202

Series:

GSE14921

6 Samples

Download data: TIFF, TXT

(Submitter supplied) Kupffer cells have been implicated in the pathogenesis of various liver diseases. However, their involvement in metabolic disorders of the liver, including fatty liver disease, remains unclear. The present study sought to determine the impact of Kupffer cells on hepatic triglyceride storage and to explore the possible mechanisms involved. To that end, C57Bl/6 mice rendered obese and steatotic by chronic high-fat feeding were treated for 1 week with clodronate liposomes, which cause depletion of Kupffer cells. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4166

Platform:

GPL1261

4 Samples

Download data: CEL

Analysis of liver from C57BL/6 males fed high fat diets (HFD) for 20 wks to induce obesity and hepatic steatosis, and injected with clodronate during the last week of HFD to deplete Kupffer cells (KCs) in liver. Results provide insight into role of KCs in regulation of hepatic triglyceride storage.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 disease state, 2 growth protocol, 2 protocol sets

Platform:

GPL1261

Series:

GSE33044

4 Samples

Download data: CEL

(Submitter supplied) transcriptomic changes in wild typw and PPARa-null mice fed the Lieber-Decarli liquid diet with or without alcohol for up to four months to identify biomarkers for the early stage of alcohol induced liver damage. Mice were sampled after 1, 2 and 4 months treatment.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10787

48 Samples

Download data: TXT

(Submitter supplied) Perfluoroalkyl substances (PFAS) are man-made chemicals with suspected endocrine-disrupting properties. Exposure to perfluorooctanoic acid (PFOA) has been linked to disturbed metabolism via the liver, although the exact mechanism is not clear. Moreover, information on the metabolic effects of the new PFAS alternative GenX is limited. We tested whether low-dose exposure to PFOA and GenX induces metabolic disturbances, including NAFLD, dyslipidemia, and glucose tolerance in mice and studied the involvement of PPARα. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23479

32 Samples

Download data: RDATA, TXT

(Submitter supplied) The effect of ablation of Tak1 gene in the liver was characterized.

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5474

Platform:

GPL6885

4 Samples

Download data: TXT

Analysis of liver from 4-week old C57BL/6 males with hepatocyte-specific deletion of TGFβ-activated kinase 1 (TAK1). In hepatocytes, TAK1 controls activation of both IKK/NF-κB and JNK pathways. Results provide insight into the role of TAK1 in liver pathophysiology.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation sets

Platform:

GPL6885

Series:

GSE51962

4 Samples

Download data

(Submitter supplied) Caloric Restriction in Leptin Deficiency Worsens Myocardial Steatosis: Failure to Upregulate PPAR gamma and Thermogenic Glyecrolipid/Fatty Acid Cycling Growing evidence supports an anti-lipotoxic role for leptin in preventing inappropriate peripheral tissue lipid deposition. Obese, leptin deficient ob/ob mice develop left ventricular (LV) hypertrophy and myocardial steatosis with increased apoptosis and decreased longevity. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

18 Samples

Download data: CEL

(Submitter supplied) Gene expression in livers of wild type adult male mice and in lncRNA 3930402G23Rik ("G23Rik") knockdown adult male mouse liver was assayed by RNA-seq, as part of a study of liver transcriptomic changes in response to activation of PPARA by WY-14,643, published in Molecular and Cellular Endocrinology (2022). These studies found that G23Rik-null mice were more susceptible to hepatic lipid accumulation in response to acute fasting. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21493

12 Samples

Download data: XLSX

(Submitter supplied) Fatty acid transport protein 2 (FATP2) is highly expressed in liver, small intestine, and kidney where it functions in both the uptake of exogenous long chain fatty acids (LCFAs) and in the activation to CoA thioesters of very long chain fatty acids (VLCFAs). Here we address the phenotypic impacts of deleting FATP2 followed by an unbiased RNA-seq analysis of the liver transcriptome. Wild type (C57BL/6J) and fatp2 null (fatp2-/-) mice (5 weeks old) were maintained on a standard chow diet for 6 weeks (11 weeks old). more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27637

16 Samples

Download data: XLSX

(Submitter supplied) Sirtuins are a family of protein deacetylases, deacylases, and ADP-ribosyltransferases that regulate life span, control the onset of numerous age-associated diseases, and mediate metabolic homeostasis. We have uncovered a novel role for the mitochondrial sirtuin SIRT4 in the regulation of hepatic lipid metabolism during changes in nutrient availability. We show that SIRT4 levels decrease in the liver during fasting and that SIRT4 null mice display increased expression of hepatic peroxisome proliferator activated receptor (PPAR ) target genes associated with fatty acid catabolism. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4823

Platform:

GPL1261

12 Samples

Download data: CEL, TXT