Format
Items per page
Sort by

Send to:

Choose Destination

Links from GEO DataSets

Items: 20

1.

Dicer Partner Proteins Tune the Length of Mature miRNAs in Flies and Mammals

(Submitter supplied) Drosophila Dicer-1 produces microRNAs (miRNAs) from pre-miRNA, whereas Dicer-2 generates small interfering RNAs (siRNAs) from long dsRNA. loquacious (loqs) encodes three Dicer partner proteins, Loqs-PA, Loqs-PB, and, Loqs-PD, generated by alternative splicing. To understand the function of each Loqs isoform, we constructed loqs isoform-specific rescue flies. Loqs-PD promotes siRNA production in vivo by Dicer-2. more...
Organism:
Mus musculus; Drosophila melanogaster
Type:
Non-coding RNA profiling by high throughput sequencing
Platforms:
GPL9061 GPL9250
24 Samples
Download data: TXT
Series
Accession:
GSE37443
ID:
200037443
2.

Reconstitution of the RNAi response in human cells using drosophila gene products

(Submitter supplied) While mammalian somatic cells are incapable of mounting an effective RNA interference (RNAi) response to viral infections, plants and invertebrates are able to generate high levels of functional short interfering RNAs (siRNAs) of viral origin that can effectively control many infections. In Drosophila, the RNAi response is mediated by the Dicer 2 enzyme (dDcr2) acting in concert with two co-factors called Loqs-PD and R2D2. more...
Organism:
Homo sapiens
Type:
Non-coding RNA profiling by high throughput sequencing; Expression profiling by high throughput sequencing
Platform:
GPL11154
12 Samples
Download data: BED
Series
Accession:
GSE83433
ID:
200083433
3.

Derivation and characterization of Dicer and microRNA deficient human cells

(Submitter supplied) We have used genome editing to generate inactivating deletion mutations in all three copies of the dicer (hdcr) gene present in the human cell line 293T. As previously shown in murine ES cells lacking Dicer function, hDcr-deficient 293T cells are severely impaired for the production of mature microRNAs (miRNAs). Nevertheless, RNA-induced silencing complexes (RISCs) present in these hDcr-deficient cells are readily programmed by transfected, synthetic miRNA duplexes to repress mRNAs bearing either fully or partially complementary targets, including targets bearing incomplete seed homology to the introduced miRNA. more...
Organism:
Homo sapiens
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL11154
14 Samples
Download data: CSV, TSV
Series
Accession:
GSE56836
ID:
200056836
4.

The small RNA profile in wildtype, R2D2 and loquacious mutant Drosophila

(Submitter supplied) We used SOLiD technology to deep sequence libraries prepared from 18-35 nt PAGE purfied small RNAs derived from female Drosophila. The objective of our study was to compare the impact of R2D2 or loquacious mutantions on different small RNA populations in the fruit fly.
Organism:
Drosophila melanogaster
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL9521
3 Samples
Download data: TXT
Series
Accession:
GSE18871
ID:
200018871
5.

Processing of Drosophila endo-siRNAs depends on a specific Loquacious isoform

(Submitter supplied) Drosophila melanogaster expresses three classes of small RNAs, which are classified according to their mechanisms of biogenesis. MicroRNAs are ~22-23-nt, ubiquitously expressed small RNAs that are sequentially processed from hairpin-like precursors by Drosha/Pasha and Dcr-1/Loquacious complexes. MicroRNAs usually associate with AGO1 and regulate the expression of protein-coding genes. Piwi-interacting RNAs (piRNAs) of ~24-28-nt associate with Piwi-family proteins and can arise from single-stranded precursors. more...
Organism:
Drosophila melanogaster
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL9061
6 Samples
Download data: CSV
Series
Accession:
GSE17171
ID:
200017171
6.

small RNAs produced in the wild-type and mutant Dicer-2 transgenic flies

(Submitter supplied) Examine the abundance and length of siRNAs produced by mutant Dicer-2 in vivo and in vitro in order to understand the mechanism by which Dicer-2 produces highly precise 21 nt siRNAs.
Organism:
Drosophila melanogaster; synthetic construct
Type:
Non-coding RNA profiling by high throughput sequencing
Platforms:
GPL21306 GPL21616
12 Samples
Download data: TXT
Series
Accession:
GSE84532
ID:
200084532
7.

Intertwined pathways for Argonaute-mediated microRNA biogenesis in Drosophila

(Submitter supplied) Ago1-IP small RNA sequencing from S2R+ cells, with dsRNA knockdown of Dicer1 (and GFP control)
Organism:
Drosophila melanogaster
Type:
Other; Non-coding RNA profiling by high throughput sequencing
Platform:
GPL13304
2 Samples
Download data: TXT
8.

Loqs-PD and R2D2 define independent pathways for RISC generation in Drosophila

(Submitter supplied) In Drosophila, siRNAs are classified as endo- or exo-siRNAs based on their origin. Both are processed from double-stranded RNA precursors by Dcr-2, then loaded into the Argonaute protein Ago2. While exo-siRNAs serve to defend the cell against viruses, endo-siRNAs restrict the spread of selfish DNA in somatic cells, analogous to piRNAs in the germ line. Endo- and exo-siRNAs display a differential requirement for double-stranded RNA binding domain proteins (dsRBPs): R2D2 is needed to load exo-siRNAs into Ago2 while the PD isoform of Loquacious (Loqs-PD) stimulates Dcr-2 during the nucleolytic processing of hairpin-derived endo-siRNAs. more...
Organism:
Drosophila melanogaster
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL9061
4 Samples
Download data: FA, MAP, TXT
Series
Accession:
GSE26230
ID:
200026230
9.

small RNAs produced in the mutant Dicer-2 transgenic flies

(Submitter supplied) Examine the abundance and length of siRNAs produced by mutant Dicer-2 in vivo and in vitro in order to understand the mechanism by which Dicer-2 produces highly precise 21 nt siRNAs.
Organism:
synthetic construct; Drosophila melanogaster
Type:
Non-coding RNA profiling by high throughput sequencing
Platforms:
GPL21616 GPL21306
4 Samples
Download data: TXT
Series
Accession:
GSE94803
ID:
200094803
10.

The effects of the 5' pocket motif of Dicer on miRNA biogenesis

(Submitter supplied) A hallmark of RNA silencing is a class of ~22 nt RNAs which are processed from dsRNA precursor by Dicer. Accurate processing by Dicer is critical for the functionality of microRNAs (miRNAs). According to the current model, Dicer measures the length by anchoring the 3' overhang of the dsRNA terminus. Here we find that human Dicer binds to the 5' end of RNA and utilizes the 5' end as an additional reference point for cleavage site selection (5' counting rule). more...
Organism:
Mus musculus
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL9250
5 Samples
Download data
Series
Accession:
GSE27903
ID:
200027903
11.

Ovary small RNA

(Submitter supplied) We sequenced small RNA from total RNA samples in two siRNA-pathway mutants in fly ovaries. This submission comes from a modENCODE project. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
Organism:
Drosophila melanogaster
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL9058
2 Samples
Download data
Series
Accession:
GSE25570
ID:
200025570
12.

ago2[414] ovary total RNA

(Submitter supplied) modENCODE_submission_2834 This submission comes from a modENCODE project of Eric Lai. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We plan to generate a comprehensive catalog of expressed and functional microRNAs, and generate biological evidence for their regulatory activity. We plan also to delineate the primary transcription units of microRNA genes. Finally, we plan to annotate other classes of non-miRNA expressed small RNAs, as least some of which may define novel classes of small RNA genes. more...
Organism:
Drosophila melanogaster
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL9058
1 Sample
Download data: SAM
Series
Accession:
GSE24317
ID:
200024317
13.

dcr-2[L811fsX] Ago1 Immunoprecipitation ovary

(Submitter supplied) modENCODE_submission_2833 This submission comes from a modENCODE project of Eric Lai. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We plan to generate a comprehensive catalog of expressed and functional microRNAs, and generate biological evidence for their regulatory activity. We plan also to delineate the primary transcription units of microRNA genes. Finally, we plan to annotate other classes of non-miRNA expressed small RNAs, as least some of which may define novel classes of small RNA genes. more...
Organism:
Drosophila melanogaster
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL9058
1 Sample
Download data: SAM
Series
Accession:
GSE24316
ID:
200024316
14.

r2d2 Ago1 Immunoprecipitation ovary

(Submitter supplied) modENCODE_submission_2749 This submission comes from a modENCODE project of Eric Lai. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We plan to generate a comprehensive catalog of expressed and functional microRNAs, and generate biological evidence for their regulatory activity. We plan also to delineate the primary transcription units of microRNA genes. Finally, we plan to annotate other classes of non-miRNA expressed small RNAs, as least some of which may define novel classes of small RNA genes. more...
Organism:
Drosophila melanogaster
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL9058
1 Sample
Download data: SAM
Series
Accession:
GSE24313
ID:
200024313
15.

r2d2 ovary total RNA

(Submitter supplied) modENCODE_submission_2748 This submission comes from a modENCODE project of Eric Lai. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We plan to generate a comprehensive catalog of expressed and functional microRNAs, and generate biological evidence for their regulatory activity. We plan also to delineate the primary transcription units of microRNA genes. Finally, we plan to annotate other classes of non-miRNA expressed small RNAs, as least some of which may define novel classes of small RNA genes. more...
Organism:
Drosophila melanogaster
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL9058
1 Sample
Download data: SAM
Series
Accession:
GSE24312
ID:
200024312
16.

Ago2[414] Ago1 Immunoprecipitation

(Submitter supplied) modENCODE_submission_2746 This submission comes from a modENCODE project of Eric Lai. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We plan to generate a comprehensive catalog of expressed and functional microRNAs, and generate biological evidence for their regulatory activity. We plan also to delineate the primary transcription units of microRNA genes. Finally, we plan to annotate other classes of non-miRNA expressed small RNAs, as least some of which may define novel classes of small RNA genes. more...
Organism:
Drosophila melanogaster
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL9058
1 Sample
Download data: SAM
Series
Accession:
GSE24311
ID:
200024311
17.

Canton S Ago1 Immunoprecipitation

(Submitter supplied) modENCODE_submission_2744 This submission comes from a modENCODE project of Eric Lai. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We plan to generate a comprehensive catalog of expressed and functional microRNAs, and generate biological evidence for their regulatory activity. We plan also to delineate the primary transcription units of microRNA genes. Finally, we plan to annotate other classes of non-miRNA expressed small RNAs, as least some of which may define novel classes of small RNA genes. more...
Organism:
Drosophila melanogaster
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL9058
1 Sample
Download data: SAM
Series
Accession:
GSE24310
ID:
200024310
18.

Deep sequencing of Drosophila melanogaster small RNAs

(Submitter supplied) High throughput seqeuncing of small RNAs (PAGE isolated from total RNA or Argonaute immunoprecipitates) from Drosophila melanogaster using the Illumina platform. Adapter ligation requires 5' monophosphate and 3' OH. Full analysis of all libraries in this set is published (Czech B. et al. 2008), leading to the description of endogenous siRNAs in flies. Keywords: Solexa sequences
Organism:
Drosophila melanogaster; Flock House virus
Type:
Non-coding RNA profiling by high throughput sequencing
Platforms:
GPL9058 GPL9402
9 Samples
Download data: TXT
Series
Accession:
GSE11086
ID:
200011086
19.

Drosophila melanogaster small RNA sequence: cell lines and imaginal disc

(Submitter supplied) High-throughput sequencing of Drosophila melanogaster small RNAs. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Keywords: High-throughput solexa sequencing
Organism:
Drosophila melanogaster
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL9058
4 Samples
Download data
Series
Accession:
GSE10794
ID:
200010794
20.

Re-evaluation of the roles of DROSHA, Exportin 5, and DICER in microRNA biogenesis

(Submitter supplied) Biogenesis of canonical microRNAs (miRNAs) involves multiple steps: nuclear processing of primary miRNA (pri-miRNA) by DROSHA, nuclear export of precursor miRNA (pre-miRNA) by Exportin 5 (XPO5), and cytoplasmic processing of pre-miRNA by DICER. To gain a deeper understanding of the contribution of each of these maturation steps, we deleted DROSHA, XPO5, and DICER in the same human cell line, and analyzed their effects on miRNA biogenesis. more...
Organism:
Homo sapiens
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL15520
9 Samples
Download data: TXT
Series
Accession:
GSE77989
ID:
200077989
Format
Items per page
Sort by

Send to:

Choose Destination

Supplemental Content

db=gds|term=|query=1|qty=6|blobid=MCID_614c7c5aaf6db32b316e68e9|ismultiple=true|min_list=5|max_list=20|def_tree=20|def_list=|def_view=|url=/Taxonomy/backend/subset.cgi?|trace_url=/stat?
   Taxonomic Groups  [List]
Tree placeholder
    Top Organisms  [Tree]

Find related data

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Support Center