GEO DataSets

(Submitter supplied) The negative feedback mechanism is essential to maintain effective immunity and tissue homeostasis. 1,25-dihydroxyvitamin D (1,25(OH)2D3) modulates innate immune response, but the mechanism remains poorly understood. Here we report that vitamin D receptor (VDR) signaling attenuates Toll-like receptor-mediated inflammation by enhancing the negative feedback inhibition. VDR inactivation leads to a hyperinflammatory response in mice and macrophage cultures when challenged with lipopolysaccharide (LPS) due to miR-155 overproduction that excessively suppresses SOCS1, a key regulator that enhances the negative feedback loop. more...

Organism:

Murid gammaherpesvirus 4; Betapolyomavirus hominis; Mus musculus; Rattus norvegicus; Murid betaherpesvirus 1; JC polyomavirus; Human immunodeficiency virus 1; Human gammaherpesvirus 8; Homo sapiens; Human alphaherpesvirus 1; Human betaherpesvirus 5; human gammaherpesvirus 4; Betapolyomavirus macacae

Type:

Non-coding RNA profiling by array

Platform:

GPL7723

6 Samples

Download data: GPR

(Submitter supplied) Gene expression profiling of macrophages derived from WT and Vdr deficient mice after stimulation with IFNgamma and/or 1alpha,25(OH)2D3 Keywords = macrophages Keywords = VDR Keywords = activated vitamin D3 Keywords = IFNgamma Keywords = KO mice Keywords: dose response

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1389

Platform:

GPL339

8 Samples

Download data: CEL, CHP, RPT

Analysis of wild type and vitamin D receptor (Vdr) knockout primary bone marrow-derived macrophages following treatment with IFN-gamma and/or 1alpha,25-dihydroxyvitamin D3 (1alpha,25(OH)2D3). Results indicate that 1alpha,25(OH)2D3 suppresses effector functions of IFN-gamma-activated macrophages.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 agent, 2 genotype/variation sets

Platform:

GPL339

Series:

GSE2421

8 Samples

Download data: CEL, CHP, RPT

(Submitter supplied) Purpose:The purpose of this study is to detect activated or silenced genes during LPS-induced dendritic cell maturation. Gene expression differences between two samples could be found using transcriptome profiling (RNA-seq) analysis. Methods:Mouse dendritic cells were generated from bone marrow cells in RPMI-1640 medium with recombinant mouse GM-CSF and IL-4, mature DCs were obtained after LPS induced maturation. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

2 Samples

Download data: TXT

(Submitter supplied) In this study, the transcriptome of peripheral blood mononuclear cells (PBMCs) of a healthy adult was investigated in response to in vitro treatment with LPS, beta-glucan and 1,25(OH)2D3.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

54 Samples

Download data: XLSX

(Submitter supplied) In this study, we focused on the time-course of transcriptional changes in freshly isolated human PBMCs 4, 8, 24 and 48 h after onset of stimulation with the active vitamin D metabolite 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) compared to vehicle (0.1% EtOH).

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

24 Samples

Download data: XLSX

(Submitter supplied) In this study we have analysed the regulation of miRNA in bone marrow derived macrophages in response to the fungal pathogen heat killed Candida albicans and bacterial cell wall component, LPS. The aim of the study was to identify and validate miRNAs involved in the innate immune system in response to fungal and bacterial stimuli and investigate potential mechanisms for their transcription.

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array

Platform:

GPL10458

5 Samples

Download data: XLS

(Submitter supplied) We generated myeloid cells lacking VDR (KODMAC) by crossing Vdrfl/fl mice with lysosome-M-promoter-driven Cre mice (Lyz2Cre) in the Ldlr-/-background (a model of diet-induced metabolic syndrome), as previously described13 and compared them to Vdrfl/flLdlr-/- littermates (control). Unbiased miRNA expression analyses using media from KODMAC or control peritoneal macrophages identified 361 differentially expressed miRNAs with p<0.05

Organism:

Mus musculus; synthetic construct

Type:

Non-coding RNA profiling by array

Platform:

GPL19117

18 Samples

Download data: CEL

(Submitter supplied) Chronic inflammation is known to contribute to the development of hypertension, but while the role of lymphocytes is well-established, the myeloid lineage, particularly as a possible mediator of renin-mediated hypertension, has not been studied. Vitamin D deficiency has pro-inflammatory consequences in monocytes, and it is linked to renin-mediated hypertension in mice. This prompted us to test the hypothesis that conditional knockout of the vitamin D receptor (VDR) in macrophages (KODMAC) would promote renin-dependent hypertension. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

16 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus; Homo sapiens

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing; Other

Platforms:

GPL18573 GPL19057

56 Samples

Download data: NARROWPEAK