GEO DataSets

(Submitter supplied) Dendritic cell (DC) are critical initiators and regulators of immunity to pathogens, vaccines, tumors and tolerance to self. Mouse and human dendritic cells (DCs) are comprised of functionally specialized subsets, but precise interspecies correlation is currently incomplete and hampers the full translation of murine findings to human DC-based clinical therapies. In this study, we show that murine lung and gut lamina propria CD11b+ DC populations are comprised of two subsets: FLT3- and IRF4-dependent CD24+CD64- DCs and contaminating CSF-1R-dependent CD24-CD64+ macrophages. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

6 Samples

Download data: TXT

(Submitter supplied) This file contains gene microarray data from subsets of human intestinal dendritic cells, as defined by their expression of CD103 and Sirpa. This will allow for better understanding of human intestinal DC subsets in general and will facilitate translation from findings in the mouse.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

11 Samples

Download data: CEL, CHP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus; Homo sapiens

Type:

Expression profiling by array; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL7202 GPL13112

41 Samples

Download data: TXT, WIG

(Submitter supplied) Purpose: The purpose of this study is to identify the genome-wide binding sites for IRF4 interaction partners PU.1, BATF, and JunB in dendritic cells. These ChIP-seq data were integrated with gene expression analysis in IRF4-sufficient and -deficient BMDCs in order to assemble an IRF4 gene regulatory network.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13112

3 Samples

Download data: WIG

(Submitter supplied) Hematopoietic progenitors from mouse bone marrow were differentiated into dendritic cells for 5 days with GM-CSF and IL-4. On day 6, MHCIIhi and MHCIIlo BMDC subpopulations were sorted to purify by flow cytometry. RNA was extracted and processed for microarray analysis.

Organism:

Homo sapiens; Mus musculus

Type:

Expression profiling by array

Platform:

GPL7202

8 Samples

Download data: TXT

(Submitter supplied) Hematopoietic progenitors from mouse bone marrow were differentiated into dendritic cells for 5 days with GM-CSF and IL-4. On day 6, MHCIIhi and MHCIIlo BMDC subpopulations were sorted to purify by flow cytometry. RNA was extracted and processed for microarray analysis.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7202

30 Samples

Download data: TXT

(Submitter supplied) Assessing the gene expression repertoire of antigen presenting dendritic cells Keywords: other

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL81

7 Samples

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(Submitter supplied) sorted hematopoetic stem cells from bone marrow; with two rounds of amplification Keywords: repeat sample

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL81

2 Samples

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(Submitter supplied) FLT3+/CD11b+ Dendritic cell (DC) progenitor was amplified in vitro from mouse bone marrow. Keywords: repeat sample

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL81

5 Samples

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(Submitter supplied) FLT3+CD11b+ DC progenitor was amplified in vitro from mouse bone marrow. DC were differentiated using GM-CSF and analyzed at day 7 and day 10 of differentiation and after 16h TNFalpha stimulation at day 10 Keywords: time-course

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2440

Platform:

GPL32

4 Samples

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Analysis of dendritic cell (DC) development from Flt3(+)CD11b(+) DC progenitors. DC progenitors treated with GM-CSF for 7 and 10 days to induce differentiation. DCs differentiated for 10 days were subsequently treated with TNFalpha to induce maturation.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 protocol sets

Platform:

GPL32

Series:

GSE575

4 Samples

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(Submitter supplied) Mouse lung CD11c+ dendritic cells are composed of 2 major DC subsets, the CD103+CD11b-low/intermediate DC (CD103+ DC) and the CD11b-highCD103- DC (CD11b-high DC). These 2 subsets are functionally distinct. Comparison of their functions showed CD103+ DC Microarray analysis was performed to compare the gene expression profiles of the 2 lung DC subsets in naïve mice.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL

(Submitter supplied) Atopic asthma is a chronic inflammatory disease of the lungs that is commonly associated with a Th2 response. The role of allergen-specific IgG in the initiation and development of allergic airway inflammation is still poorly understood; however, a receptor of IgG-immune complexes, CD16, has been demonstrated to promote augmentation of Th2 responses. To identify what genes downstream of CD16 signaling may be contributing to development of a Th2 response, we use ovalbumin (OVA) as our model antigen and compared wildtype and CD16-/- BMDCs that were treated overnight with OVA or OVA-immune complex.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2995

12 Samples

Download data: TXT

(Submitter supplied) Dendritic cells (DCs) in tissues and lymphoid organs comprise distinct functional subsets that differentiate in situ from circulating progenitors. Tissue-specific signals that regulate DC subset differentiation are poorly understood. We report that DC-specific deletion of the Notch2 receptor caused a reduction of DC populations in the spleen. Within the splenic CD11b+ DCs, Notch signaling blockade ablated a distinct population marked by high expression of adhesion molecule Esam. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

3 Samples

Download data: CEL, CHP

(Submitter supplied) DC, monocyte and macrophage networks are evolutionarily conserved but the distinct subsets have been difficult to distinguish due to shared overlapping phenotypic markers between the cells. Using transcriptome microarray profiling of human and mouse mononuclear phagocyte subsets, we have distinguished human dermal DCs from monocyte-derived cells and macrophages.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5349

Platform:

GPL6884

11 Samples

Download data: TXT

Analysis of FACS-sorted dermal macrophage, epidermal Langerhans and CD14+ dendritic cell (DC) subsets. DCs, monocytes and macrophages are leukocytes with critical roles in immunity. Result provide insight into molecular profiles distinguishing dermal DCs from monocyte-derived cells and macrophages.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 3 cell type, 6 individual sets

Platform:

GPL6884

Series:

GSE60317

11 Samples

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(Submitter supplied) Pulmonary dendritic cells are heterogenous cells comprise four distinct subsets including two conventional dendritic cell subsets, CD103+ and CD11bhiCD14lo cells, and two monocyte-derived dendritic cell subsets. Their functions in terms of migration and T cell activation are distinct, but genes regulating their features are to be determined. We used microarrays to identify a select set of genes that are expressed in conventinal dendritic cells and in monocyte-derived dendriti cells.

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5663

Platform:

GPL1261

12 Samples

Download data: CEL, CHP

Analysis of four dendritic cell (DC) subsets purified from the lungs of C57BL/6 males following inhalation of LPS/OVA. The four subsets represent conventional DCs (cDCs) and monocyte-derived DCs (moDCs). Results provide insight into molecular profiles that would discriminate between cDCs and moDCs.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 cell type, 2 other sets

Platform:

GPL1261

Series:

GSE64896

12 Samples

Download data: CEL, CHP

(Submitter supplied) Comparison of gene expression changes of FACS sorted splenic CD11b+CD8a- and CD11b-CD8a+ cDC subsets reconstituted in vivo following total body irradiation in combination with exogenous retinoic acid or vehicle control.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

16 Samples

Download data: CEL

(Submitter supplied) We report phenotypes in gut dendritic cell development by loss of Runx/Cbfb transcription factor complexes. To further examine function of residual CD103+CD11b- and CD103-CD11b+ DCs, we performed RNA-seq analyses and compared gene expression signatures between control and Cbfb-deficient cells.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

12 Samples

Download data: TXT