GEO DataSets

(Submitter supplied) Phenotypically comparing the hiPSC-EpSCs with hiPSCs and hEpSCs.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5638

Platform:

GPL10558

7 Samples

Download data: TXT

Analysis of induced pluripotent stem cell-derived epithelial stem cells (hiPSC-EpSCs). The hiPSCs, which were derived from adult dermal fibroblasts, and hEpSCs directly isolated from hair follicles also examined. Results provide insight into molecular similarities between hiPSC-EpSCs and hEpSCs.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 cell type, 2 tissue sets

Platform:

GPL10558

Series:

GSE51028

7 Samples

Download data

(Submitter supplied) Androgenetic alopecia (AGA) or common baldness results from a marked decrease in hair follicle size. This miniaturization may be related to loss of hair follicle stem or progenitor cells. To test this hypothesis, we analyzed bald and non-bald scalp from the same individuals for the presence of hair follicle stem and progenitor cells using flow cytometry to quantitate cells expressing CYTOKERATIN 15 (KRT15), CD200, CD34 and ALPHA-6-INTEGRIN (ITGA6). more...

Organism:

Homo sapiens; Mus musculus

Type:

Expression profiling by array

Platforms:

GPL1261 GPL570

18 Samples

Download data: CEL, CHP, XLS

(Submitter supplied) Mouse back skin was disassociated to single cells, sorted by cell surface markers and tested by microarrray To compare the gene expression of mouse bulge (CD34+CD200+CD49+) versus secondary hair germ (CD34-CD200+CD49+) versus interfollicular epidermis (CD34-CD200-CD49+) xx Bald scalp retains hair follicle stem cells but lacks CD200-rich and CD34-positive hair follicle progenitor cells Androgenetic alopecia (AGA) or common baldness results from a marked decrease in hair follicle size. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

12 Samples

Download data: CEL, CHP

(Submitter supplied) Human hair follicles from normal areas of the scalp were disassociated to single cells, sorted and tested by microarrray To compare the expression of human CD200+ CD49+ hair follicle keratinocytes versus CD200-CD49+ keratinocytes

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

6 Samples

Download data: CEL, CHP

(Submitter supplied) The human hair follicle bulge is an important niche for keratinocyte stem cells (KSC). Elucidation of human bulge cell biology could be facilitated by analysis of global gene expression profiles and identification of unique cell surface markers. The lack of distinctive bulge morphology in human hair follicles has hampered studies of bulge cells and KSC. In this study, we determined the distribution of label-retaining cells to carefully define the human anagen bulge. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1672

Platform:

GPL96

16 Samples

Download data: CEL

Comparison of outer root sheath (ORS) cells of the hair follicle bulge with other defined ORS cell subpopulations within the follicle. Results provide insight into the biological distinctiveness of hair follicle bulge cells, suggesting the bulge is a repository for keratinocyte stem cells (KSCs).

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 5 cell type, 2 tissue sets

Platform:

GPL96

Series:

GSE3419

16 Samples

Download data: CEL

(Submitter supplied) Mammalian epidermis consists of three self-renewing compartments: the hair follicle, sebaceous gland and interfollicular epidermis. We generated knock-in alleles of murine Lgr6, a close relative to the Lgr5 stem cell gene. Lgr6 was expressed in the earliest embryonic hair placodes. In adult hair follicles, Lgr6+ cells resided in a previously uncharacterized region directly above the follicle bulge. They expressed none of the known bulge stem cell markers. Prenatal Lgr6+ cells established the hair follicle, sebaceous gland and interfollicular epidermis. Postnatally, Lgr6+ cells generated sebaceous gland and interfollicular epidermis, while contribution to hair lineages gradually diminished with age. Adult Lgr6+ cells executed long-term wound repair, including the formation of new hair follicles. We conclude that Lgr6 marks the most primitive epidermal stem cell.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4134

2 Samples

Download data: TXT

(Submitter supplied) This experiment was designed to show the similarity among normal human epidermal melanocytes, melanocytes derived from human 3F-induced pluripotent stem (iPS) cells, and human 3F-iPS cells.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

3 Samples

Download data: TXT

(Submitter supplied) Clonogenic keratinocyte stem cells isolated from the bulge area of human telogen follicles were co-cultured with dermal papilla cells in a transwell system. RNA was isolated from stem cells for different periods of time (day 0, 1, 2, and 5) after co-culture with DP and analyzed for changes in gene expression using Genechip microarrays. Keywords = epithelial stem cells Keywords = DP Keywords = co-culture Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS687

Platform:

GPL8300

3 Samples

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Analysis of dermal papilla (DP) induced epithelial stem cell differentiation. Keratinocyte stem cells from bulge area of telogen hair follicle co-cultured with DP over a 5 day period.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 time sets

Platform:

GPL8300

Series:

GSE1470

3 Samples

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(Submitter supplied) In this study, we defined a differentiation approach for guiding human pluripotent stem cells in to complex hair-bearing skin tissue, known as skin organoids. The primary goal of this single-cell RNA-sequencing analysis was to define the cellular composition of skin organoids, which we have shown using histology contain multiple embryonic cell lineages. The secondary goal was to determine whether skin organoids generated using different cell lines (WA25 hESCs and DSP-GFP hiPSCs) contain similar cell types.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL24676 GPL18573

5 Samples

Download data: TAR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL17021 GPL9250 GPL16570

14 Samples

Download data: CEL, TXT, WIG

(Submitter supplied) We performed ChIP-Seq analysis of SOX10, histone H3 lysine 27 acetylation (H3K27ac) and H3K27 trimethylation (H3K27me3) in melanocytes to profile the genomic binding sites of SOX10 and the chromatin landscape. In parallel, we generated Sox10 haploinsufficient cell lines using gene knockout approaches and conducted microarray gene expression analysis to identify functional gene targets of SOX10 transcriptional regulation in melanocytes. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL9250 GPL17021

8 Samples

Download data: TXT, WIG

(Submitter supplied) We performed ChIP-Seq analysis of SOX10, histone H3 lysine 27 acetylation (H3K27ac) and H3K27 trimethylation (H3K27me3) in melanocytes to profile the genomic binding sites of SOX10 and the chromatin landscape. In parallel, we generated Sox10 haploinsufficient cell lines using gene knockout approaches and conducted microarray gene expression analysis to identify functional gene targets of SOX10 transcriptional regulation in melanocytes. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

6 Samples

Download data: CEL

(Submitter supplied) Amniotic epithelial stem cells (AESCs) are considered potential alternatives for keratinocytes (KCs) in tissue-engineered skin substitutes. However, their clinical application to skin regeneration is hindered as AESCs and KCs are not identical. Herein, transcriptomics analysis were used to understand the commonalities and differences between human AESCs and KCs. RNA-sequencing revealed that AESCs are involved in multiple epidermis-associated biological processes, which are shared by KCs, and show more similarity to early-stage immature KCs than adult KCs. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

6 Samples

Download data: TXT

(Submitter supplied) We identify a combination of small molecules to be able to transdifferentiate dermal cells to muscle cells. To characterize the cellular diversity of the neonatal dermal we performed scRNA-seq on mononuclear cell suspensions obtained from the neonatal dermal. Following normalization and quality control of scRNA-seq data, we captured 40537 cells with an average of 5755 genes expressed per cell. We charted the entire course of skin-to-muscle chemical reprogramming by single cell RNA-sequencing to determine the source cells in CD73- cells from skin with the potential to be transdifferentiated to muscle cells .We found that melanocytes could be chemically reprogrammed to myogenic lineage cells.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

5 Samples

Download data: CSV, MTX, TSV