GEO DataSets

(Submitter supplied) To further characterize the role of the unliganded glucocorticoid receptor (GR) in breast cells, we used an shRNA vector directed against GR to create EPH-4 mouse mammary cell lines with depleted endogenous levels of this receptor. RNA prepared from normal (EV-50) and GR-depleted (shGR-19) cells was used in an expression microarray screen for targets of unliganded GR. This analysis revealed 260 targets of negaive regulation by unliganded GR, and 343 targets of positive regulation by unliganded GR, several of which were involved in pro-apoptotic networks, and opposed the activity of liganded GR targets. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7202

4 Samples

Download data: TXT

(Submitter supplied) A mutation in the dimerization domain of the mouse glucocorticoid receptor (GR), dim1, has recently been shown to bind DNA and regulate gene expression. To expand these studies we created a stable osteosarcoma (U-2 OS) cell line expressing four mutations in the dimerization domain of the human GR, dim4 (N454D, A458T, R460D, D462C), and used whole human genome microarray analysis to compare differences in gene regulation between vehicle treated (CON) and those treated with the glucocorticoid receptor agonist dexamethasone (DEX) at 100nM concentration for 6 hours.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4133

6 Samples

Download data: TXT

(Submitter supplied) Glucocorticoids regulate diverse physiologic processes and synthetic derivatives of these natural hormones are widely used in the treatment of inflammatory diseases. However, chronic administration often triggers insensitivity and serious side effects including osteoporosis. The underlying mechanisms regulating these side effects are not completely understood. We report here that human osteosarcoma U-2 OS bone cells lacking the glucocorticoid receptor (GR) are resistant to glucocorticoid killing whereas the expression of wild-type GR activates an apoptotic program. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3432

Platform:

GPL1708

60 Samples

Download data: TIFF, TXT

Analysis of glucocorticoid receptor alpha (hGRalpha) isoform -A, -B, -C, or -D expressing osteosarcoma cells for up to 24 h after hGRalpha activation with dexomethasone. Cell apoptosis occurred in a GR isoform-selective manner. Results provide insight into the function of each isoform.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 5 agent, 4 time sets

Platform:

GPL1708

Series:

GSE6711

60 Samples

Download data: TIFF, TXT

(Submitter supplied) This series contain time course microarray data from MCF10A-Myc cells treated with either ethanol or Dexamethasone for 30 min, 2 hr, 4 hr, and 24 hr. This series contains three biological replicates that were analyzed as independent replicate experiments (data were normalized within each replicate experiment, not across all samples). Keywords: time course

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS2095 GDS2096 GDS2097

Platform:

GPL96

24 Samples

Download data: CEL

Analysis of breast cancer MCF10A-Myc cells at various time points up to 24 hours following treatment with dexamethasone to activate the glucocorticoid receptor (GR). GR activation is critical in the stress response of virtually all cell types.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 4 time sets

Platform:

GPL96

Series:

GSE4917

8 Samples

Download data: CEL

Analysis of breast cancer MCF10A-Myc cells at various time points up to 24 hours following treatment with dexamethasone to activate the glucocorticoid receptor (GR). GR activation is critical in the stress response of virtually all cell types.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 4 time sets

Platform:

GPL96

Series:

GSE4917

8 Samples

Download data: CEL

Analysis of breast cancer MCF10A-Myc cells at various time points up to 24 hours following treatment with dexamethasone to activate the glucocorticoid receptor (GR). GR activation is critical in the stress response of virtually all cell types.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 4 time sets

Platform:

GPL96

Series:

GSE4917

8 Samples

Download data: CEL

(Submitter supplied) The objective of this study was to dertemine GR binding patterns within wild type murine embryonic fibroblasts in comparison to embryonic fibroblasts derived from mice containing a null mutation for Klf15.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13112

7 Samples

Download data: BED

(Submitter supplied) In this work, we used our recently developed method Spec-seq to characterize the binding specificity of Glucocorticoid receptor in vitro.

Organism:

Homo sapiens

Type:

Other

Platform:

GPL15520

2 Samples

Download data: TXT

(Submitter supplied) The glucocorticoid receptor (GR) regulates adaptive transcriptional programs that alter metabolism in response to stress. Network properties that allow GR to tune gene expression to match specific physiologic demands are poorly understood. We analyzed the transcriptional consequences of GR activation in murine lungs deficient for Klf15, a transcriptional regulator of amino acid metabolism that is induced by glucocorticoids and fasting

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7202

29 Samples

Download data: TXT

(Submitter supplied) Analysis of MCF-7 cells treated for 4h with Ethanol, Estradiol (E2), Dexamethasone (Dex), or Estradiol + Dexamethasone (E2 + Dex) In estrogen receptor (ER)-negative breast cancer (BC), high tumor glucocorticoid receptor (GR) expression has been associated with a relatively poor outcome. In contrast, using a meta-analysis of several genomic datasets, here we find that tumor GR mRNA expression is associated with improved ER+ relapse-free survival (RFS) (independently of progesterone receptor (PR) expression). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

12 Samples

Download data: CEL

(Submitter supplied) Glucocorticoids are critical components of combination chemotherapy regimens in pediatric acute lymphoblastic leukemia (ALL). However, the signaling pathways regulating apoptosis in glucocorticoid-treated lymphoid cells remain unclear. In this study, pediatric ALL patient-derived xenograft inherently sensitive to glucocorticoids were exposed to dexamethasone in vivo. Whole-genome GR binding sites and histone acetylation status were detected using chromatin immunoprecipitation sequencing analyses. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL11154

6 Samples

Download data: BED, BW

(Submitter supplied) Glucocorticoids are critical components of combination chemotherapy regimens in pediatric acute lymphoblastic leukemia (ALL). The pro-apoptotic BIM protein is an important mediator of glucocorticoid-induced apoptosis in normal and malignant lymphocytes, while the anti-apoptotic BCL2 confers resistance. The signaling pathways regulating BIM and BCL2 expression in glucocorticoid-treated lymphoid cells remain unclear. more...

Organism:

Mus musculus; Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6884

58 Samples

Download data: TXT

(Submitter supplied) The glucocorticoid and progesterone receptors (GR and PR) are closely related members of the steroid receptor family. Despite sharing similar structural and functional characteristics; the cognate hormones display very distinct physiological responses. In mammary epithelial cells, PR activation is associated with the incidence and progression of breast cancer, whereas the GR is related to growth suppression and differentiation. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9052

8 Samples

Download data: BED

(Submitter supplied) Glucocorticoids are a class of steroid hormones that bind to and activate the Glucocorticoid Receptor, which then positively or negatively regulates transcription of many genes that govern multiple important physiological pathways such as inflammation and metabolism of glucose, fat and bone. Previous studies focusing on single coregulators demonstrated that each coregulator is required for regulation of only a subset of all the genes regulated by a steroid hormone. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

40 Samples

Download data: TXT

(Submitter supplied) The objective of this study was to determine binding patterns for GR, 65 and RNAP2 in Beas-2B airway epithelial cells after treatment with dexamethasone (100 nm), TNF (20 ng/ml) or both for one hour.

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL11154

21 Samples

Download data: BED

(Submitter supplied) The glucocorticoid receptor (GR) recruits many coregulators via the well characterized AF2 interaction surface in the GR ligand binding domain, but LIM domain coregulator Hic-5 binds to the relatively uncharacterized tau2 activation domain in the hinge region of GR. Requirement of Hic-5 for glucocorticoid-regulated gene expression in U2OS osteosarcoma cells was defined by Hic-5 depletion and global gene expression analysis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5269

Platform:

GPL10558

48 Samples

Download data: TXT

Analysis of U2OS-GR osteosarcoma cells (which stably express glucocorticoid receptor α) depleted for hydrogen peroxide-inducible clone-5 (Hic-5) then treated with the GR ligand dexamethasone for up to 24hrs. Results provide insight into the role of LIM domain coregulator Hic-5 in GR signaling.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent, 3 protocol, 4 time sets

Platform:

GPL10558

Series:

GSE46448

48 Samples

Download data

(Submitter supplied) Neurosphere cultures prepared from E14.5 mouse cerebral cortex at passage 3 were treated for 4 hours with 100 nM dexamethasone We used microarrays to detail the global program of dexamethasone regulated gene expression in embryonic neural progenitor/stem cells

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5256

Platform:

GPL8321

20 Samples

Download data: CEL