GEO DataSets

(Submitter supplied) Nanoemulsion adjuvant affects immune gene expression in dendritic cells. Microarray was used to asses global changes in gene expression in JAWS II dendritic cells upon treatment with nanoemulsion (NE) adjuvant W805EC

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5601

Platform:

GPL1261

10 Samples

Download data: CEL, TXT

Analysis of JAWS II DCs incubated with nanoemulsion (NE) adjuvant W805EC for up to 24 hrs. W805EC is an oil-in-water emulsion with nanodroplets similar in size to many viruses infecting the nasal mucosa. Results provide insight into the molecular basis of the adjuvant activity of W805EC NE in DCs.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 5 protocol, 2 time sets

Platform:

GPL1261

Series:

GSE51804

10 Samples

Download data: CEL

(Submitter supplied) Analysis of the effect on dendritic cells of the injection in C57BL/6 mice of VLPs or ncRNAVLPs at gene expression level.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

11 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of macrophages (of various genetic backgrounds) infected with L. monocytogenes or transfected with purified ligands Keywords: Timecourse, cell type comparison, bacteria strain comparison, drug comparison, ligand comparison.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL5137

81 Samples

Download data: GPR

(Submitter supplied) Transcriptional profiling of WT macrophages transfected with L. monocytogenes genomic DNA, synthetic MDP, or both Keywords: Ligand comparison.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL5137

8 Samples

Download data: GPR

(Submitter supplied) Transcriptional profiling of WT macrophages infected with WT L. monocytogenes for 180 minutes, with or without CAPE treatment Keywords: Drug comparison.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL5137

8 Samples

Download data: GPR

(Submitter supplied) Transcriptional profiling of WT and irf3-/- macrophages infected with WT L. monocytogenes for 180 minutes Keywords: Cell type comparison.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL5137

12 Samples

Download data: GPR

(Submitter supplied) Transcriptional profiling of WT and myd88-/- macrophages infected with WT and hly- L. monocytogenes for 30, 60, 120, or 180 minutes Keywords: Timecourse, cell type comparison, bacteria strain comparison.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL5137

53 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

8 Samples

Download data: CEL

(Submitter supplied) Antigen uptake, processing and presentation by dendritic cells are regulated by complex intra- and inter-cellular signalling events. Typical vaccine adjuvants lead to the transcription of pro-inflammatory cytokines and chemokines which relate to immune induction. We used microarrays to detail the global expression of genes in BMDC underlying emulsion internalization and DC activation with a non-inflammatory nanoemulsion adjuvant.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

4 Samples

Download data

(Submitter supplied) Antigen uptake, processing, trafficking and presentation in nasal mucosal tissues are regulated by complex intra- and inter-cellular signalling events. Typical vaccine adjuvants lead to the transcription of pro-inflammatory cytokines and chemokines which relate to immune induction. We used microarrays to detail the global expression of genes in murine nasal mucosa underlying immune induction with a non-inflammatory nanoemulsion nasal adjuvant.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

4 Samples

Download data: CEL

(Submitter supplied) Purpose: The goals of this study are to compare the different functions of manganese (II) and LPS on the activation and maturation of antigen presenting cells-BMDCs. Methods: BMDCs were generated by culturing bone marrow cells with 20 ng/ml of IL-4 (Genscript) and 20 ng/ml of GM-CSF (Genscript) at 37°C and 5% CO2 for 7 days.Then BMDCs were treated with MnCl2 (200 μM) or LPS (100 ng/ml) for 20 h. Then mRNAs were purified, sequenced and analyzed. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

3 Samples

Download data: XLSX

(Submitter supplied) Bisphosphonates are a class of drugs that are widely used to inhibit loss of bone mass in patients. We show that the administration of clinically relevant doses of bisphosphonates in mice increases antibody responses to live and inactive viruses, proteins, haptens and existing commercial vaccine formulations. Bisphosphonates exert this adjuvant-like activity in the absence of CD4+ and γδ T cells, neutrophils or dendritic cells and their effect does not rely on local macrophage depletion nor does it depend upon Toll-like receptor signaling or the inflammasome. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11180

8 Samples

Download data: CEL