Similar studies for GEO DataSets (Select 200052300) - GEO DataSets

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Items: 20

Select item 2000523001.

Chip-chip from HepG2 cells with EZH2, SUZ12 and H3K27me3

(Submitter supplied) Polycomb group (PcG) proteins including EZH2, SUZ12 and so on, which specifically catalyze trimethylation of histone 3 lysine 27 (H3K27me3), and methylated H3K27 can be recognized by other specific binding proteins to compress chromatin structure, leading to the transcriptional repression of the target genes. To completely understand the epigenetic profile and molecular network of PcG in HCC, we performed ChIP-on-chip screens with EZH2, SUZ12 and H3K27me3 antibodies in HepG2 cells.

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by genome tiling array

Platform:: GPL9448
3 Samples

Download data: GFF, JPG, PAIR, XLSX

Series

Accession:: GSE52300

ID:: 200052300

Select item 2000541082.

A cDNA microarray analysis was performed on shRNA KDs of EZH2, SUZ12, BMI1, or CBX8 HepG2 cells.

(Submitter supplied) Polycomb group (PcG) proteins including EZH2, SUZ12 ,BMI1,CBX8 and so on, which specifically catalyze trimethylation of histone 3 lysine 27 (H3K27me3), and methylated H3K27 can be recognized by other specific binding proteins to compress chromatin structure, leading to the transcriptional repression of the target genes. To explore a potential functional implication of PcG components in HCC, we stably transfected HepG2 cells with either vectors or constructs expressing shRNA that specifically targets EZH2, SUZ12, BMI1, or CBX8. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL10332
5 Samples

Download data: TXT

Series

Accession:: GSE54108

ID:: 200054108

Select item 2000688633.

Expression analysis of sk-hep-1(sk) cell and sk-hep-1-sh EZH2(sks) cell

(Submitter supplied) Investigation of EZH2 knocking down on whole genome gene expression level changes in sk-hep-1 compared to sk-hep-1-sh EZH2.

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL18943
2 Samples

Download data: CALLS, PAIR

Series

Accession:: GSE68863

ID:: 200068863

Select item 2000687584.

Genome-wide maps of H3K27me3 modification sites in sk-hep-1,sk-hep-1-shEZH2 and LO2 cells.

(Submitter supplied) We report the application of chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) for high-throughput profiling of H3K27me3 modifications in sk-hep-1(sk), sk-hep-1-shEZH2 (sks) and LO2 cells. Following standard ChIP procedure, 10 ng of each DNA sample was used for Illumina sequencing. Statistically significant ChIP-enriched regions (peaks) were identified by sk vs sks (p-value threshold of 10-2) or sk vs LO2 (p-value threshold of 10-3). more...

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL11154
3 Samples

Download data: BED, WIG, XLS

Series

Accession:: GSE68758

ID:: 200068758

PubMed Similar studies SRA Run Selector

Select item 2000177335.

Delineation of EZH2 oncogenic functions in hepatocellular carcinoma

(Submitter supplied) The goal of this study was to delineate the important EZH2 direct target genes that mediate the oncogenic properties of EZH2 in HCC. The EZH2 direct target genes in two HCC cell lines were identified by chromatin immunoprecipitation microarray (ChIP-chip) analysis and later confirmed by independent ChIP-PCR. The functions of the target genes were further examined.

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by genome tiling array

Platforms:: GPL4125 GPL4124
8 Samples

Download data: TXT

Series

Accession:: GSE17733

ID:: 200017733

Select item 2001133266.

Epigenetic reprogramming in hepatic carcinogenesis

(Submitter supplied) Genomic sequencing of hepatocellular carcinoma (HCC) uncovers a paucity of actionable mutations, underscoring the necessity to exploit epigenetic vulnerabilities for therapeutics. In HCC, EZH2-mediated H3K27me3 represents a major oncogenic chromatin modification, but how it modulates the therapeutic vulnerability of signaling pathways remains unknown. Here, we identified that EZH2 maintains H3K27 methylome through epigenetic silencing of specific gene sets. more...

Organism:: Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL18480
10 Samples

Download data: BW

Series

Accession:: GSE113326

ID:: 200113326

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2000416107.

mRNA expression after siRNA-mediated knock down of Enhancer of zeste homolog 2 (Ezh2) in human umbilical vein endothelial cells

(Submitter supplied) mRNA expression after Ezh2 knock down was analyzed to identify genes regulated by Ezh2.

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL6480
3 Samples

Download data: TXT

Series

Accession:: GSE41610

ID:: 200041610

Select item 2000313548.

The Polycomb Group Protein Suz12 Is Required for Embryonic Stem Cell Differentiation

(Submitter supplied) Polycomb group (PcG) proteins form multiprotein complexes, called Polycomb repressive complexes (PRCs). PRC2 contains the PcG proteins EZH2, SUZ12, and EED and represses transcription through methylation of lysine (K) 27 of histone H3 (H3). Suz12 is essential for PRC2 activity and its inactivation results in early lethality of mouse embryos.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL1261
4 Samples

Download data: CEL, CHP

Series

Accession:: GSE31354

ID:: 200031354

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2000274629.

Long non-coding RNAs and mRNAs expression analysis of HCC

(Submitter supplied) Although many protein-coding genes have been identified to be aberrantly expressed in hepatocellular carcinoma (HCC), the mechanisms that account for development and progression of HCC remain unclear. In recent years, long noncoding RNAs (lncRNAs) have been shown to have critical regulatory roles in mammalian cell biology. Many lncRNAs can result in aberrant expression of gene products that may contribute to cancer biology. more...

Organism:: Homo sapiens

Type:: Non-coding RNA profiling by genome tiling array

Platform:: GPL11269
10 Samples

Download data: PAIR

Series

Accession:: GSE27462

ID:: 200027462

Select item 20020256110.

DNMT and EZH2 inhibitors synergize for gene activation in hepatocellular carcinoma cells.

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens

Type:: Methylation profiling by genome tiling array; Expression profiling by high throughput sequencing

Platforms:: GPL21145 GPL18573
48 Samples

Download data: IDAT

Series

Accession:: GSE202561

ID:: 200202561

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20020256011.

DNMT and EZH2 inhibitors synergize for gene activation in hepatocellular carcinoma cells [RNA-seq]

(Submitter supplied) Hepatocellular carcinoma (HCC) is the most common primary liver malignancy with cases increasing dramatically in the USA. Developing more effective targeted therapies for HCC is an urgent need. The FDA-approved DNA methyltransferase inhibitors (DNMTis) 5-azacytidine (5-aza-CR, Vidaza) and 5-aza-2’-deoxycytidine (5-aza-CdR, Decitabine) represented the first clinical breakthrough to target aberrant cancer epigenomes. more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL18573
24 Samples

Download data: TSV

Series

Accession:: GSE202560

ID:: 200202560

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20020255912.

DNMT and EZH2 inhibitors synergize for gene activation in hepatocellular carcinoma cells [methylation array]

Organism:: Homo sapiens

Type:: Methylation profiling by genome tiling array

Platform:: GPL21145
24 Samples

Download data: IDAT, TXT

Series

Accession:: GSE202559

ID:: 200202559

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20007497413.

Global Trabscriptome Analaysis Reveals that Poly(ADP-Ribose) Polymerase 1 Regulates Gene Expression through EZH2

(Submitter supplied) Post-translational modifications, such as poly(ADP-ribosyl)ation (PARylation), regulate chromatin-modifying enzymes, ultimately affecting gene expression. This study explores the role of poly(ADP-ribose) polymerase (PARP) on global gene expression in a lymphoblastoid B cell line. We found that inhibition of PARP catalytic activity with olaparib resulted in global gene deregulation, affecting approximately 11% of genes expressed. more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL10999
9 Samples

Download data: TXT

Series

Accession:: GSE74974

ID:: 200074974

PubMed Full text in PMC Similar studies Analyze with GEO2R SRA Run Selector

Select item 20006925314.

Genome-wide profiling of histone H3 lysine 27 and lysine 4 trimethylation in multiple myeloma reveals the importance of Polycomb gene targeting and highlights EZH2 as a potential therapeutic target

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens

Type:: Expression profiling by array; Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:: GPL16558 GPL17586
22 Samples

Download data: BED, CEL, TAB

Series

Accession:: GSE69253

ID:: 200069253

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20006925115.

Genome-wide profiling of histone H3 lysine 27 and lysine 4 trimethylation in multiple myeloma reveals the importance of Polycomb gene targeting and highlights EZH2 as a potential therapeutic target [Affymetrix]

(Submitter supplied) in this study we define an epigenomic profile of PRC2 (H3K27me3 and bivalent) tragets in four newly diagnosed MM patients. Using Oncomine database we demonstarte that PRC2 targets are underexpressed with advanced ISS stages and correlated to poor outcome. Pharmacological inhibition of UNC1999 showed anti-myeloma potential in vitro by activating the expression genes related to apoptosis and cell differenatiation.

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL17586
6 Samples

Download data: CEL

Series

Accession:: GSE69251

ID:: 200069251

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Select item 20005321516.

(Submitter supplied) Multiple myeloma (MM) is a hematopoietic malignancy characterised by the accumulation of neoplastic post-germinal centre, isotype-switched, long-lived plasma cell within the bone marrow. In genetic and clinical terms MM is highly heterogeneous and remains fatal. Here we present the first epigenomic map of MM derived from freshly isolated bone marrow plasma cells from four patients. Using ChIP- and RNA-sequencing we define a set of silent H3K27me3 targets, active genes bearing H3K4me3, and bivalent genes.

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platform:: GPL16558
16 Samples

Download data: BED, TAB

Series

Accession:: GSE53215

ID:: 200053215

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20006000317.

Expression data from Control or ShSuz12 rat Intestinal epithelial cells IEC-6

(Submitter supplied) Polycomb-group proteins form multimeric protein complexes involved in transcriptional silencing. The Polycomb Repressive complex 2 (PRC2) contains the Suppressor of Zeste-12 protein (Suz12) and the histone methyltransferase Enhancer of Zeste protein-2 (Ezh2). This complex, catalyzing the di- and tri-methylation of histone H3 lysine 27, is essential for embryonic development and stem cell renewal. However, the role of Polycomb-group protein complexes in the control of the intestinal epithelial cell (IEC) phenotype is not known. more...

Organism:: Rattus norvegicus

Type:: Expression profiling by array

Platform:: GPL1355
6 Samples

Download data: CEL

Series

Accession:: GSE60003

ID:: 200060003

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20019652618.

Polycomb-mediated genome architecture enables long-range spreading of H3K27 methylation [RNA-Seq]

(Submitter supplied) Polycomb-group proteins play critical roles in gene silencing through the deposition of histone H3 lysine 27 trimethylation (H3K27me3) and chromatin compaction. This process is essential for embryonic stem cells (ESCs) pluripotency, differentiation, and development. Polycomb repressive complex 2 (PRC2) can both read and write H3K27me3, enabling processive spread of H3K27me3 on linear genome and epigenetic memory. more...