GEO DataSets

(Submitter supplied) As macrophages are the primary site of Mtb infection and are sites of vitamin D signaling, we have used these cells to understand the molecular mechanisms underlying modulation of the immune response by the hormonal form of vitamin D, 1,25-dihydroxyvitamin D (1,25D). Microarrays were used to measure the changes in gene expression induced by 1,25D treatment of H37Rv-infected THP-1 cells

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4781

Platform:

GPL6244

12 Samples

Download data: CEL, XLSX

Analysis of Mtb H37Rv-infected THP-1 macrophages treated with the hormonal form of vitamin D (1,25D). Macrophages are the primary site of Mtb infection and are sites of 1,25D signaling. Results provide insight into the molecular basis of the 1,25D-enhanced macrophage response to Mtb infection.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent, 2 infection sets

Platform:

GPL6244

Series:

GSE52819

12 Samples

Download data: CEL

(Submitter supplied) We investigated the molecular mechanisms by which colchicine regulates host defense in macrophages against M. tuberculosis infection. To elucidate this, we performed global gene expression analysis of M. tuberculosis infected-bone marrow-derived macrophages after colchicine treatment.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

9 Samples

Download data: TXT

(Submitter supplied) Previous reports have shown low vitamin D serum levels and polymorphisms in the vitamin D receptor (VDR) to be associated with increased risk for TB. Given that 1α,25-dihydroxyvitamin D3 has a role in lipid metabolism control, we tested whether the link between 1α,25-dihydroxyvitamin D3 and tuberculosis involves macrophage lipid metabolism. Since formation of lipid droplets (LD) is a hallmark of lipid dysregulation in M. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16686

14 Samples

Download data: CEL

(Submitter supplied) A role for vitamin A in host defense against Mycobacterium tuberculosis has been suggested through epidemiological and in vitro studies; however, the antimicrobial mechanism is unclear. Here, we demonstrate that vitamin A mediates host defense through regulation of cellular cholesterol content. Comparison of monocytes stimulated with all-trans retinoic acid (ATRA) or 1,25-dihydroxyvitamin D3, the biologically active forms of vitamin A and vitamin D respectively, indicates that ATRA and 1,25D3 induce mechanistically distinct antimicrobial activities. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4860

Platform:

GPL570

12 Samples

Download data: CEL, CHP

Analysis of monocytes stimulated with biologically active forms of vitamin A and vitamin D. Vitamin A plays a role in host defense against Mycobacterium tuberculosis. Results provide insight into molecular mechanisms underlying the antimicrobial activities for vitamin A and vitamin D in monocytes.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 agent, 4 individual sets

Platform:

GPL570

Series:

GSE46268

12 Samples

Download data: CEL, CHP

(Submitter supplied) Tuberculosis remains a major cause of death from an infectious disease worldwide, yet only 10% of people infected with Mycobacterium tuberculosis develop disease. Defining both necessary and sufficient immunologic determinants of protection remains a great scientific challenge. Analysis of peripheral blood gene expression profiles of active tuberculosis patients has identified correlates of risk for disease or pathogenesis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

32 Samples

Download data: CEL

(Submitter supplied) This study characterizes the genetic basis of variation in the immunomodulatory effects of the active form of vitamin D, 1,25-dihydroxyvitamin D3 (1,25D). This was done by mapping quantitative traits of 1,25D response both at the cellular and transcriptional level in peripheral blood mononuclear cells (PBMCs).

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

170 Samples

Download data: TXT

(Submitter supplied) This experiment is to compare the transcription patterns of mouse macrophages (J774A.1) infected with BCG, H37Ra and M. smegmatis under high multiplicity of infection (MOI). Through the global transcriptome profiling study, we define a pathogen specific host gene expression pattern and indicate that SRC likely plays a central role in regulating multiple unique signaling pathways activated by MTB infection. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5634

Platform:

GPL1261

4 Samples

Download data: CEL, CHP

Analysis of macrophage cell line J774A.1 infected with pathogenic mycobacteria BCG and H37Ra and non-pathogenic M. smegmatis under high multiplicity of infection (MOI = 50). Results provide insight into the molecular response of macrophage host cells to pathogenic and non-pathogenic mycobacteria.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 infection, 4 species sets

Platform:

GPL1261

Series:

GSE45675

4 Samples

Download data: CEL, CHP

(Submitter supplied) Tuberculosis (TB) caused by infection with Mycobacterium tuberculosis (Mtb) is characterized by inflammatory pathology and poorly understood mechanisms of innate immunity. Pattern recognition receptors (PRR), expressed on the surface of macrophages, determine the balance of inflammatory and antimicrobial functions that influence disease outcome. Carbohydrate moieties displayed by mycobacteria can serve as PRR ligands for some members of the C-type lectin receptor (CLR) family; interactions that mediate a variety of incompletely understood immune outcomes. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21626

30 Samples

Download data: TSV

(Submitter supplied) Total RNA from PBMCs isolated from TB patients and healthy individuals was subjected to RNASeq analysis. The samples among two groups were compared to analyze differentially expressed genes.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

9 Samples

Download data: CSV

(Submitter supplied) Co-culture of platelets with monocytes induces the forming of multinucleated giant foam cells (MP-Mac or MP-MNGCs). Compared with normal monocyte differentiated macrophages (M-Mac), the MP-MNGCs can engulf more mycobacteria with suppressed inflammatory responses (releasing more IL-10 with less TNF-gamma). Microarray analysis demonstrated that the MP-MNGCs greatly up-regulated CXCL5, PPBP, NT5E, PDPN, CXCL3 and other M2 related genes, such as ARG1, CXCL1, CD163, etc. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4133

4 Samples

Download data: TXT

(Submitter supplied) Autophagy is a conserved lysosomal-dependent cellular degradation process shown to play a key role in immune defense against Mycobacterium tuberculosis inside host macrophages. Induction of autophagy enhances mycobacterial phagosome acquisition of lysosomal hydrolases, resulting in the destruction of intracellular M. tuberculosis reference strain H37Rv and strains belonging to the East African Indian genotype. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

12 Samples

Download data: CSV

(Submitter supplied) Mouse RAW264.7 cells were stimulated with 2 uM purfied recombinant Mycobacterial EST12 protein for 0 h or 3 h

Organism:

Mus musculus

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platform:

GPL21700

4 Samples

Download data: TXT

(Submitter supplied) Target genes of transcription factor Myc regulated by Mycobacterium tuberculosis EST12

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL24247

2 Samples

Download data: BW, TXT

(Submitter supplied) Monocytes and monocyte-derived macrophages (MDM) from blood circulation infiltrate and promote glioblastoma growth by, at least partially, expressing pro-inflammatory cytokine IL-1β . Using SC-RNA seq, we analyze how IL1a and IL1b differentially contibute to tumor growth.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

9 Samples

Download data: MTX, TSV