Similar studies for GEO DataSets (Select 200054322) - GEO DataSets

Select item 2000543221.

RNA-seq analysis in Cornea epithelial cells (CECs), skin epithelial cells (SECs), LSCs after knocking down PAX6 (3-D shPAX6 LSCs) and SESCs transduced with PAX6(3-D PAX6+ SESCs) upon 3-D differentiation

(Submitter supplied) Purpose: We find that Wnt7a-PAX6 axis determine corneal epithelial cell fate. To obtain global evidence for successful cell fate conversion, we performed gene expression profiling by RNA-seq on CECs, SECs, and LSCs after knocking down PAX6 and on SESCs transduced with PAX6 upon 3-D differentiation. Methods: Under 3-D culture condition, limbal stem cell (LSCs) can be differentiated to Cornea epithelial cells (CECs), and skin epithelial stem cells (SESCs) can be differentiated to skin epithelial cells (SECs). more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL11154
8 Samples

Download data: TXT

Series

Accession:: GSE54322

ID:: 200054322

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Select item 2001562732.

Core transcription regulatory circuitry orchestrates stratified epithelial homeostasis

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL20795
44 Samples

Download data: XLSX

Series

Accession:: GSE156273

ID:: 200156273

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Select item 2001562723.

Core transcription regulatory circuitry orchestrates stratified epithelial homeostasis (ChIP-seq, ATAC-seq)

(Submitter supplied) Adult stem cell identity, plasticity, and homeostasis are precisely orchestrated by lineage-restricted epigenetic and transcriptional regulatory networks. Here, by integrating super-enhancer and chromatin accessibility landscapes, we delineate core transcription regulatory circuitries (CRCs) of limbal stem/progenitor cells (LSCs) and find that RUNX1 and SMAD3 are required for maintenance of the corneal epithelial identity and homeostasis. more...

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL20795
32 Samples

Download data: XLSX

Series

Accession:: GSE156272

ID:: 200156272

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Select item 2001562714.

Core transcription regulatory circuitry orchestrates stratified epithelial homeostasis (RNA-seq)

(Submitter supplied) Adult stem cell identity, plasticity, and homeostasis are precisely orchestrated by lineage-restricted epigenetic and transcriptional regulatory networks. Here, by integrating super-enhancer and chromatin accessibility landscapes, we delineate core transcription regulatory circuitries (CRCs) of limbal stem/progenitor cells (LSCs) and find that RUNX1 and SMAD3 are required for maintenance of the corneal epithelial identity and homeostasis. more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL20795
12 Samples

Download data: XLSX

Series

Accession:: GSE156271

ID:: 200156271

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Select item 2002429955.

Multi-omics analysis identifies coordination and hierarchy of transcription factors controlling specific epithelial cell fates in corneal epithelium [scRNA-seq]

(Submitter supplied) The homeostasis of the transparent corneal epithelium in the eye is maintained by the proliferation and differentiation of limbal stem cells that possess the proper cell fate. A potential disease mechanism underlying corneal opacity has been proposed to be limbal stem cells acquiring the cell fate of keratinocytes in the non-transparent epidermis. In this study, we performed a multi-omics analysis of human limbal stem cells derived from the cornea and keratinocytes from the epidermal stratum basale and characterized the similar yet distinct molecular signatures of these cells. more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL18573
2 Samples

Download data: MTX, TSV, TXT

Series

Accession:: GSE242995

ID:: 200242995

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Select item 2002429906.

Multi-omics analysis identifies coordination and hierarchy of transcription factors controlling specific epithelial cell fates in corneal epithelium [Bulk RNA-seq]

(Submitter supplied) The homeostasis of the transparent corneal epithelium in the eye is maintained by the proliferation and differentiation of limbal stem cells that possess the proper cell fate. A potential disease mechanism underlying corneal opacity has been proposed to be limbal stem cells acquiring the cell fate of keratinocytes in the non-transparent epidermis. In this study, we performed a multi-omics analysis of human limbal stem cells derived from the cornea and keratinocytes from the epidermal stratum basale and characterized the similar yet distinct molecular signatures of these cells. more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL18573
14 Samples

Download data: TSV

Series

Accession:: GSE242990

ID:: 200242990

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Select item 2002364407.

Multi-omics analyses identify transcription factor interplay in corneal epithelial fate determination and disease [CUT&RUN]

(Submitter supplied) The transparent corneal epithelium in the eye is maintained through the homeostasis regulated by limbal stem cells, while the non-transparent epidermis relies on epidermal keratinocytes for renewal. Despite their cellular similarities, the precise cell fates of these two types of epithelial stem cells, which give rise to functionally distinct epithelia, remain unknown. We performed a multi-omics analysis of human limbal stem cells from the cornea and keratinocytes from the epidermis and characterized their molecular signatures, highlighting their similarities and differences. more...

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL18573
3 Samples

Download data: BW, XLS

Series

Accession:: GSE236440

ID:: 200236440

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Select item 2002069248.

Multi-omics analyses identify transcription factor interplay in corneal epithelial fate determination and disease

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL18573
47 Samples

Download data: BW, MTX, NARROWPEAK, TXT, XLS

Series

Accession:: GSE206924

ID:: 200206924

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Select item 2002069239.

Multi-omics analyses identify transcription factor interplay in corneal epithelial fate determination and disease [scRNA-seq]

(Submitter supplied) The transparent corneal epithelium in the eye is maintained through the homeostasis regulated by limbal stem cells, while the non-transparent epidermis relies on epidermal keratinocytes for renewal. Despite their cellular similarities, the precise cell fates of these two types of epithelial stem cells, which give rise to functionally distinct epithelia, remain unknown. We performed a multi-omics analysis of human limbal stem cells from the cornea and keratinocytes from the epidermis and characterized their molecular signatures, highlighting their similarities and differences. more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL18573
5 Samples

Download data: MTX, TAB, TXT

Series

Accession:: GSE206923

ID:: 200206923

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Select item 20020692210.

Multi-omics analyses identify transcription factor interplay in corneal epithelial fate determination and disease [RNA-seq]

(Submitter supplied) The transparent corneal epithelium in the eye is maintained through the homeostasis regulated by limbal stem cells, while the non-transparent epidermis relies on epidermal keratinocytes for renewal. Despite their cellular similarities, the precise cell fates of these two types of epithelial stem cells, which give rise to functionally distinct epithelia, remain unknown. We performed a multi-omics analysis of human limbal stem cells from the cornea and keratinocytes from the epidermis and characterized their molecular signatures, highlighting their similarities and differences. more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL18573
4 Samples

Download data: TSV

Series

Accession:: GSE206922

ID:: 200206922

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Select item 20020692011.

Multi-omics analyses identify transcription factor interplay in corneal epithelial fate determination and disease [ChIP-seq]

(Submitter supplied) The transparent corneal epithelium in the eye is maintained through the homeostasis regulated by limbal stem cells, while the non-transparent epidermis relies on epidermal keratinocytes for renewal. Despite their cellular similarities, the precise cell fates of these two types of epithelial stem cells, which give rise to functionally distinct epithelia, remain unknown. We performed a multi-omics analysis of human limbal stem cells from the cornea and keratinocytes from the epidermis and characterized their molecular signatures, highlighting their similarities and differences. more...

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL18573
9 Samples

Download data: BW, XLS

Series

Accession:: GSE206920

ID:: 200206920

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Select item 20020691812.

Multi-omics analyses identify transcription factor interplay in corneal epithelial fate determination and disease [ATAC-seq]

(Submitter supplied) The transparent corneal epithelium in the eye is maintained through the homeostasis regulated by limbal stem cells, while the non-transparent epidermis relies on epidermal keratinocytes for renewal. Despite their cellular similarities, the precise cell fates of these two types of epithelial stem cells, which give rise to functionally distinct epithelia, remain unknown. We performed a multi-omics analysis of human limbal stem cells from the cornea and keratinocytes from the epidermis and characterized their molecular signatures, highlighting their similarities and differences. more...

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL18573
10 Samples

Download data: BW, NARROWPEAK

Series

Accession:: GSE206918

ID:: 200206918

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Select item 20011300813.

mRNA sequencing of Aniridia limbal epithelial cells.

(Submitter supplied) PAX6-related Aniridia is a sight-threatening disease due to progression of secondary glaucoma and aniridia associated keratopathy (AAK). Changes or loss of limbal epithelial progenitors causes the epithelial surfaces defects. We analyzed how PAX6 contribute to this with a two-step approach. 1) mRNA Sequencing of limbal epithelial cells isolated from controls and aniridia patients. 2) confirming the bioinformatical and literature-based result list on a siRNA based primary aniridia cell model (PAX6- knockdown). more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL16791
4 Samples

Download data: BW

Series

Accession:: GSE113008

ID:: 200113008

Select item 20021514914.

RNA-seq of SPARC-treated human corneal epithelial cells

(Submitter supplied) We identified that Sparc gene expression is upregulated in corneal epithelial cells in a mouse model of dry eye disease involving lacrimal gland excision. Therefore, in this experiment we assess the effect of SPARC treatment on the transcriptome of human corneal epithelial cells.