GEO DataSets

(Submitter supplied) Increased small airway resistance and decreased lung elasticity contribute to the airflow limitation in chronic obstructive pulmonary disease (COPD). The lesion that corresponds to loss of lung elasticity is emphysema; the small airway obstruction is due to inflammatory narrowing and obliteration. Despite their convergence in altered physiology, different mechanisms contribute to these processes. The relationships between gene expression and these specific phenotypes may be more revealing than comparison with lung function. We measured the ratio of alveolar surface area to lung volume (SA/V) in lung tissue from 43 smokers. Two samples from 21 subjects, in which SA/V differed by > 49 cm2/mL were profiled to select genes whose expression correlated with SA/V. The level of expression of 181 transcripts was related to SA/V ( p< 0.05). The relationship between expression of these transcripts and SA/V was tested in the 22 remaining subjects as a replication. Thirty of the 181 genes remained significantly associated with SA/V (P<0.05) and the direction of association was the same in 164/181. Pathway and network analysis revealed enrichment of genes involved in protein ubiquitination, and western blotting showed altered expression of genes involved in protein ubiquitination in obstructed individuals. These data implicate modified protein ubiquitination and degradation as a potentially important pathway in the pathogenesis of emphysema. Raw data not available for this study

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL3991

42 Samples

Download data: TXT

(Submitter supplied) Exploratory design using pooled samples of 3 patients with emphysema and 3 patients without COPD Aim: 1.Search for expressed genes to select for a custom designed array (200 genes, n=50). 2. Find highly differentially expressed genes as targets for qPCR 3. Search for regulated senescent associated genes Keywords: disease comparison

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL3016

2 Samples

Download data: GEL, GMD, TIFF, TXT

(Submitter supplied) Comparison of emphysema vs non emphysema COPD lung tissue expression

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13667

70 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10379

1349 Samples

Download data: CEL

(Submitter supplied) We profiled genome-wide gene expression in non-tumorous human lung tissues. The overall goal of this project is to improve our molecular understanding of various lung diseases including lung cancer and chronic obstructive pulmonary disease (COPD).

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10379

445 Samples

Download data: CEL

(Submitter supplied) We profiled genome-wide gene expression in non-tumorous human lung tissues. The overall goal of this project is to improve our molecular understanding of various lung diseases including lung cancer and chronic obstructive pulmonary disease (COPD).

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10379

405 Samples

Download data: CEL

(Submitter supplied) We profiled genome-wide gene expression in non-tumorous human lung tissues. The overall goal of this project is to improve our molecular understanding of various lung diseases including lung cancer and chronic obstructive pulmonary disease (COPD).

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10379

499 Samples

Download data: CEL

(Submitter supplied) This series represents alveolar macrophages from a mouse model of emphysema, deletion of the integrin beta6 Keywords: parallel sample

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1874

Platform:

GPL1792

15 Samples

Download data: TIFF

(Submitter supplied) This series represents isolated alveolar macrophages from human subjects. Keywords: parallel sample

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1269

Platform:

GPL570

45 Samples

Download data: CEL

Analysis of alveolar macrophages of integrin beta-6 (Itgb6) deficient animals treated with doxycycline. Itgb6 mutants develop emphysema. Effect of TGFbeta transgene induction to suppress the effect of the Itgb6 mutation also examined.

Organism:

Mus musculus

Type:

Expression profiling by array, log ratio, 3 genotype/variation sets

Platform:

GPL1792

Series:

GSE2255

15 Samples

Download data: TIFF

Analysis of alveolar macrophages from 15 cigarette smokers, 15 non-smokers and 15 asthmatics. Results suggest that alveolar macrophage activation induced by smoking contributes to emphysema.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 agent sets

Platform:

GPL570

Series:

GSE2125

45 Samples

Download data: CEL

(Submitter supplied) COPD is a common and disabling lung disease for which very few therapeutic options are currently available. We reasoned that global gene expression profiling of COPD lungs could reveal previously unidentified disease pathways for potential therapeutic interventions. Forty-eight human lung samples were obtained from lungs or lobes resected for small peripheral lung lesions (5 non-smokers, 21 GOLD 0, 9 GOLD 1, 10 GOLD 2 and 3 GOLD 3 patients). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL3991

48 Samples

Download data: XLS

(Submitter supplied) Induced sputum is used to sample inflammatory cells, predominantly neutrophils and macrophages, from the airways of COPD patients. Our aim was to identify candidate genes associated with the degree of airflow obstruction and the extent of emphysema by expression profiling, and then to confirm these findings for selected candidates using specific PCR and protein analysis.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4265

Platform:

GPL570

143 Samples

Download data: CEL

Analysis of sputum from ex-smokers with moderate (stage 2) or severe (stages 3, 4) chronic obstructive pulmonary disease (COPD). Induced sputum contains inflammatory cells, predominantly neutrophils and macrophages. Results provide insight into molecular mechanisms underlying COPD progression.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 disease state sets

Platform:

GPL570

Series:

GSE22148

143 Samples

Download data: CEL

(Submitter supplied) Chronic obstructive pulmonary disease (COPD) is a major public health problem. The aim of this study was to identify genes involved in emphysema severity in COPD patients. Gene expression profiling was performed on total RNA extracted from non-tumor lung tissue from 30 smokers with emphysema. Class comparison analysis based on gas transfer measurement was performed to identify differentially expressed genes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL3877

30 Samples

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