GEO DataSets

(Submitter supplied) The glucocorticoid receptor (GR) regulates gene expression upon activation by glucocorticoid (GC) hormones. In zebrafish, two GR splice variants exist: the canonical GR α-isoform (GRα), and the GR β-isoform (GRβ). The exact function of GRb remains elusive. We have investigated the transcriptional role of GRa and GRb in the zebrafish embryo model by injecting mebryos with two splice-blocking morpholinos (one leading to knockdown of both GR α- and β-isoforms, and another targeting the alternative splicing of the GR pre-mRNA in favor of the GR β-isoform) and with GRβ mRNA (resulting in specific GRβ overexpression). more...

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL15180

24 Samples

Download data: TXT

(Submitter supplied) In the present study, we have used the zebrafish to investigate the functional role of the glucocorticoid receptor beta-isoform (GRbeta). A transgenic zebrafish line, Tg(hsp70:Gal4, UAS:EGFP-grbeta), was generated, showing heat shock-inducible expression of a GFP-tagged GRbeta. After heat shock treatment at 1 and 2 days post fertilization (dpf), 3 dpf larvae (control and transgenic) were treated with beclomethasone. more...

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL15180

16 Samples

Download data: TXT

(Submitter supplied) Glucocorticoids with different chemical structures but similar glucocorticoid receptor potency regulate subsets of common and unique genes in human trabecular meshwork cells. Gene expression changes of human trabecular meshwork cells, TM 86 and TM 93, due to treatment with dexamethasone (Dex), fluocinolone acetonide (FA), and triamcinolone acetonide (TA).

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

23 Samples

Download data: TXT

(Submitter supplied) In zebrafish, ovulated oocytes contain both cortisol deposited from the maternal circulation and maternal mRNA for the glucocorticoid receptor (gr mRNA), which is spread as granular structures throughout the central ooplasm. At the 1-cell stage (0.2 hpf), this transcript is relocated by streamers in the blastodisc area and equally partitioned among blastomeres. At 15 hpf, it is replaced by the zygotic transcript. more...

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL6457

4 Samples

Download data: TXT

(Submitter supplied) To further characterize the roles of cortisol signaling via the glucocorticoid receptor (GR) in developing zebrafish, we have used morpholino oligonucleotides to knockdown GR protein translation and measured gene expression in RNA extracted from 24 and 36 hours post fertilization (hpf) embryos. The GR morpholino was characterized previously in Nesan et al., 2012, Endocrinology 181, 35-44)

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL7301

12 Samples

Download data: TXT

(Submitter supplied) ARGLU1 was identified in a screen for new modulators of glucocorticoid signaling in the CNS. RNA-seq of neuronal cells ±siARGLU1 revealed significant changes in the expression and alternative splicing of distinct genes involved in neurogenesis. Treatment with dexamethasone, a GR activator, also induced changes in the pattern of alternatively spliced genes, highlighting an underappreciated global mechanism of glucocorticoid action in neuronal cells. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

4 Samples

Download data: TXT

(Submitter supplied) To investigate the effects of glucocorticoids on the gene expression profiles in zebrafish, we performed a microarray-based transcriptomic study using larvae exposed to three representative glucocoriticoids at environmentally relevant high and low concentrations. Transcriptiomic profiel of developing zebrafish larvae exposed to dexamethasone, prednisolone or triamcinolone at 50 pM to 50 nM from 3 hours post-fertilisation to 5 days post-fertilisation were analyzed using G2519F Agilent Zebrafish Whole Genome Oligo Microarray Ver3.0, 4x44K.

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL14664

34 Samples

Download data: TXT

(Submitter supplied) We found that a frameshift deletion introduced into exon 1 of zebrafish klf9 eliminates the DNA binding domain and significantly reduces expression of the mature transcript. RNA-seq shows that klf9 is required for the pro-inflammatory transcriptomic effects of chronic cortisol treatment. klf9 is one of a core set of genes consistently found to be upregulated by chronic cortisol exposure.

Organism:

Danio rerio

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20828

16 Samples

Download data: TXT

(Submitter supplied) We found that GR mediates most of the effects of the treatment, and paradoxically, that the transcriptome of cortisol-treated larvae is more like that of larvae lacking a GR than that of larvae with a GR, suggesting that the cortisol-treated larvae develop GR resistance.

Organism:

Danio rerio

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20828

16 Samples

Download data: TXT

(Submitter supplied) The Glucocorticoid Receptor (GR) is a transcriptional regulator which co-ordinates behavioural, metabolic and immune responses to stressors. Previous studies have shown that wild-type and grs357 mutant zebrafish exhibit a range of behavioural differences, implying possible roles for GR function in the brain. As part of a study to elucidate the mechanisms underlying the behaviours that are regulated by GR, we compared the brain transcriptomes of wild-type and grs357 mutant adults. more...

Organism:

Danio rerio

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24995

12 Samples

Download data: TXT

(Submitter supplied) The methylomes of whole adult zebrafish brains was compared in gr s357 mutants and wild type siblings

Organism:

Danio rerio

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL18413

4 Samples

Download data: COV

(Submitter supplied) In addition to satisfying the metabolic demands of cells, mitochondrial metabolism helps regulate immune cell function. To date, such cell-intrinsic metabolic-immunologic cross-talk has only been described operating in cells of the immune system. Here we show that epidermal cells utilize fatty acid β-oxidation to fuel their contribution to the immune response during cutaneous inflammation. By live imaging metabolic and immunological processes within intact zebrafish embryos during cutaneous inflammation, we uncover a mechanism where elevated β-oxidation-fueled mitochondria-derived reactive oxygen species within epidermal cells helps guide matrix metalloproteinase-driven leukocyte recruitment. more...

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL1319

6 Samples

Download data: CEL