GEO DataSets

(Submitter supplied) Recent technological advances have made transcriptome sequencing (RNA-seq) possible in cells with low RNA copy number including platelets. Resulting studies have used RNA-seq in platelets isolated from healthy individuals to characterize the platelet transcriptome. However, platelets, possibly through gene expression changes, contribute to the etiology of and response to cardiovascular disease and events. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

34 Samples

Download data: CSV, XLS

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL6106 GPL6884

57 Samples

Download data

(Submitter supplied) We aim to determine blood transcriptome-based molecular signature of acute coronary syndrome (ACS), and to identify novel serum biomarkers for early stage ST-segment-elevation myocardial infarction (STEMI)

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6106

24 Samples

Download data: TXT

(Submitter supplied) We aim to determine blood transcriptome-based molecular signature of acute coronary syndrome (ACS), and to identify novel serum biomarkers for early stage ST-segment-elevation myocardial infarction (STEMI)

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6884

33 Samples

Download data: TXT

(Submitter supplied) Platelets are anucleate cytoplasmic fragments that lack genomic DNA, but continue to synthesize protein using a pool of mRNAs, ribosomes, and regulatory small RNAs inherited from the precursor megakaryocyte (MK). The regulatory processes that shape the platelet transcriptome and the full scope of platelet translation have remained elusive. Using RNA-Seq and ribosome profiling of primary human platelets, we show the platelet transcriptome encompasses a subset of transcripts detected by RNA-Seq analysis of in vitro derived MK cells and these platelet-enriched transcripts are broadly occupied by ribosomes. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

14 Samples

Download data: TXT

(Submitter supplied) Acute myocardial infarction (AMI) is primarily due to coronary atherosclerotic plaque rupture and subsequent thrombus formation. Platelets play a key role in the genesis and progression of both atherosclerosis and thrombosis. Since platelets are anuclear cells that inherit their mRNA from megakaryocyte precursors and maintain it unchanged during their life span, gene expression (GE) profiling at the time of an AMI provides information concerning the platelet GE preceding the coronary event. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17586

57 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL18573

14 Samples

Download data: TXT

(Submitter supplied) To compare immature and mature platelet transcriptome Methods: reticulated or immature platelets(RPs) were defined as the platelets with the highest thiazole orange staining intensity (15% highest, RNAhigh) according to previous experiences and mature platelets were defined as the platelet with the 30% lowest thiazole orange staining intensity (RNAlow) and sorted (supplemental Figure S1D). RPs have a prothrombotic transcriptomic profile

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL18573

6 Samples

Download data: TXT

(Submitter supplied) To compare immature and mature platelet transcriptome Methods: reticulated or immature platelets(RPs) were defined as the platelets with the highest thiazole orange staining intensity (15% highest, RNAhigh) according to previous experiences and mature platelets were defined as the platelet with the 30% lowest thiazole orange staining intensity (RNAlow) and sorted (supplemental Figure S1D). We detected more than 1700 differentially expressed gene, incluning activation receptors RPs have a prothrombotic transcriptomic profile

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

8 Samples

Download data: TXT

(Submitter supplied) For the anucleate platelet it has been unclear how well platelet transcriptomes correlate among different donors or across different RNA profiling platforms, and what the transcriptomes’ relationship is with the platelet proteome. We generated RNA-seq pro-files of the long RNA transcriptomes from the platelets of 10 healthy young males (5 white and 5 black) with median age of 24.5 years, no notable clinical history, and no pre-vious history of thrombosis or bleeding. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

10 Samples

Download data: CEL

(Submitter supplied) Despite a substantial progress in diagnosis and therapy, acute myocardial infarction (MI) is a major cause of mortality in the general population. A novel insight into the pathophysiology of myocardial infarction obtained by studying gene expression should help to discover novel biomarkers of MI and to suggest novel strategies of therapy. The aim of our study was to establish gene expression patterns in leukocytes from acute myocardial infarction patients. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

98 Samples

Download data: CEL

(Submitter supplied) Whole-genome gene expression analysis has been successfully utilized to diagnose, prognosticate, and identify potential therapeutic targets for cardiovascular disease. However, the utility of this approach to identify outcome-related genes and dysregulated pathways following first-time myocardial infarction (AMI) remains unknown and may offer a novel strategy to detect affected expressome networks that predict long-term outcome. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS5022 GDS5074

Platform:

GPL570

52 Samples

Download data: CEL

Analysis of circulating blood from first-time acute myocardial infarction (AMI) patients within 48 hours of MI. Results provide insight into molecular mechanisms underlying the response of circulating cells to first-time AMI.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 disease state sets

Platform:

GPL570

Series:

GSE48060

52 Samples

Download data: CEL

Analysis of blood from first-time acute myocardial infarction (AMI) patients, clinically indistinguishable at baseline, with or without recurrent cardiovascular events at 18 months. Results provide insight into molecular mechanisms distinguishing AMI patients with or without recurrent events.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 disease state sets

Platform:

GPL570

Series:

GSE48060

31 Samples

Download data: CEL

(Submitter supplied) We report RNA-sequencing data of 779 blood platelet samples, including 402 tumor-educated platelet (TEP) samples collected from patients with non-small cell lung cancer (NSCLC). In addition, we report RNA-sequencing data of blood platelets isolated from 377 individuals without reported cancer, but not excluding individuals with inflammatory conditions. This dataset highlights the ability of TEP RNA-based 'liquid biopsy' diagnostics in patients with NSCLC.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

779 Samples

Download data: TXT, XLSX

(Submitter supplied) Platelets are small anucleate cells derived from the fragmentation of megakaryocytes and are involved in different biological processes especially hemostasis, thrombosis and immune response. Platelet purification is a crucial step in transcriptomic analysis, and researchers usually encounter the problem of platelet contamination by leukocytes and erythrocytes. Leukocytes contain much more RNA than platelets, thus the presence of few contaminants in platelet preparation can strongly alter transcriptome results. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL23126

2 Samples

Download data: CEL, CHP

(Submitter supplied) In platelets, splicing and translation occur in the absence of a nucleus. However, the integrity and stability of mRNAs derived from megakaryocyte progenitor cells remain poorly quantified on a transcriptome-wide level. As circular RNAs (circRNAs) are resistant to degradation by exonucleases, their abundance relative to linear RNAs can be used as a surrogate marker for mRNA stability in the absence of transcription. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

1 Sample

Download data: BED, BW, TXT

(Submitter supplied) In sickle cell disease, ischemia-reperfusion injury and intravascular hemolysis produce endothelial dysfunction and vasculopathy characterized by reduced nitric oxide (NO) and arginine bioavailability. Recent functional studies of platelets in patients with sickle cell disease reveal a basally activated state, suggesting that pathological platelet activation may contribute to sickle cell disease vasculopathy. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3318

Platform:

GPL570

30 Samples

Download data: CEL

Analysis of platelets from patients with sickle cell disease. Sickle cell disease patients exhibit increased activation of platelets. Results provide insight into the molecular basis of platelet dysfunction in sickle cell disease.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 disease state sets

Platform:

GPL570

Series:

GSE11524

30 Samples

Download data: CEL

(Submitter supplied) Purpose: In the current study, we used RNA-Seq to determine the transcriptional changes and understand the genetic reprogramming of the diaphragm in the chronic phase post-myocardial infarction. Methods: Rat diaphragm mRNA profiles 16 weeks post Sham and Myocardial infarction (MI) surgeries were generated by deep sequencing using llumina NextSeq 500. The sequence reads that passed quality filters were analyzed at the transcript level with TopHat followed by Cufflinks. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20084

8 Samples

Download data: CSV