GEO DataSets

(Submitter supplied) It has been known for some time that muscle repair potential becomes increasingly compromised with advancing age, and that this age-related defect is associated with reduced activity of muscle satellite cells and with the presence of chronic, low grade inflammation in the muscle. Working from the hypothesis that a heightened inflammatory tone in aged muscle could contribute to poor regenerative capacity, we developed genetic systems to inducibly alter inflammatory gene expression in satellite cells or muscle fibers by modulation of the activity of nuclear factor κB (NF-κB), a master transcriptional regulator of inflammation whose activity is upregulated in many cell types and tissues with age. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

7 Samples

Download data: CEL, CHP

(Submitter supplied) Pofut1 is an essential gene that glycosylates proteins containing EGF-like repeats, including Notch Receptors (NotchRs).  Work in mice and in Drosophila has shown that O-fucosylation by Pofut1 is required for NotchR ligands to bind to and activate NotchRs.  As such, Pofut1 deletion in skeletal myofibers allows for an analysis of potential functions and molecular changes of Pofut1 in skeletal muscle that derive from its expression in skeletal myofibers. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL

(Submitter supplied) Satellite cells are the primary source of stem cells for skeletal muscle growth and regeneration. Since adult stem cell maintenance involves a fine balance between intrinsic and extrinsic mechanisms, we performed genome-wide chronological expression profiling to identify the transcriptomic changes involved during early postnatal growth till acquisition of satellite cell quiescence.

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5660

Platform:

GPL1261

11 Samples

Download data: CEL

Analysis of myogenic stem cells called satellite cells (mSCs) FACS-sorted from the trunk of Pax3GFP/+ mice at postnatal days 1, 12, and 28, a period when most mSCs are in proliferation and quiescence states. Results provide insight into molecular basis of early postnatal skeletal muscle development.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 3 age, 2 tissue sets

Platform:

GPL1261

Series:

GSE65927

11 Samples

Download data: CEL

(Submitter supplied) (Abstract of publication submitted currently) To clarify molecular regulation of satellite cells, we performed genome-wide gene expression analysis of quiescent satellite cells isolated from mouse skeletal muscle by flow cytometry. We identified 53 novel quiescent satellite cell-specific genes whose expressions are sharply down-regulated upon activation. The gene list contains a number of cell surface molecules, transcriptional factors, and cytokines and other signal transduction molecules. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL339

12 Samples

Download data

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array; Expression profiling by high throughput sequencing

Platforms:

GPL17021 GPL10787

20 Samples

Download data: TXT

(Submitter supplied) Background and Aims: Analysis of aging-induced impairments in satellite cells (SCs) – the stem cells of skeletal muscle that are required for its regeneration. Hox genes are known to control stem cell function and development of various tissues. Methods: We used AlfpCre mice for liver specific deletion of Trp53 in a conditional knockout mouse model to analyze liver carcinogenesis. Results: Here, we show that liver-specific deletion of p53 in mice consistently induces formation of liver carcinoma depicting bilineal differentiation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10787

8 Samples

Download data: TXT

(Submitter supplied) Background and Aims: Analysis of aging-induced impairments in satellite cells (SCs) – the stem cells of skeletal muscle that are required for its regeneration. Hox genes are known to control stem cell function and development of various tissues.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

12 Samples

Download data: TXT

(Submitter supplied) A variety of epigenetic alterations impairs functions of cells and tissues during aging, but it is not known if epigenetic alterations are associated with aging muscle. Here, we examined the changes of a panel of histone marks and found H3K27ac (an active enhancer mark) is markedly increased during aging in human skeletal muscle tissues. Our integrated analysis showed that enhancer activation during muscle aging is associated with the up-regulation of extracelluar matrix (ECM) genes, which may result in stiffness of the niche environment of satellite cells (SCs). more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL15103

3 Samples

Download data: BED

(Submitter supplied) Skeletal muscle function and regenerative capacity decline during aging, yet factors driving these changes are incompletely understood. Muscle regeneration requires temporally coordinated transcriptional programs to drive myogenic stem cells to activate, proliferate, fuse to form myofibers, and to mature myonuclei, restoring muscle function after injury. We assessed global changes in myogenic transcription programs distinguishing muscle regeneration in aged mice from young mice by comparing pseudotime trajectories from single-nucleus RNA sequencing of myogenic nuclei. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL19057 GPL24247

13 Samples

Download data: H5

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19057

52 Samples

Download data: BW, CSV

(Submitter supplied) We report here the genome-wide deposition of NFAT1 (NFATc2) in wild-type and Mfn2-/- regenerating myofibers.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19057

5 Samples

Download data: BW, CSV

(Submitter supplied) We report here the genome-wide deposition of Myod and Myog in quiescent and activated muscle stem cells from wild-type mice.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19057

14 Samples

Download data: BW, CSV

(Submitter supplied) We report here the genome-wide deposition of H3K9me3 and H3K27me3 in wild-type, Mfn2-/-, Vhl-/- and HIF1dPA regenerating myofibers.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19057

25 Samples

Download data: BW, CSV

(Submitter supplied) We report the transcriptome changes in purified mouse activated muscle stem cells in response to deletion of the Mfn2 gene.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

8 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL24247 GPL21103

78 Samples

Download data

(Submitter supplied) To gain insight into the mechanisms by which exercise affects the neural stem cell compartment, we subjected young and old mice to aerobic exercise and performed single cell transcriptome analysis of cells from the subventricular zone of the brain of these animals.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

15 Samples

Download data: RDATA

(Submitter supplied) To gain insight into the mechanisms by which exercise affects the muscle stem cell compartment, we subjected young and old mice to aerobic exercise and performed single cell transcriptome analysis of mononucleated cells from hindlimb muscles of these animals.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

16 Samples

Download data: RDATA

(Submitter supplied) To gain insight into the mechanisms by which exercise affects hematopoietic stem cells, we subjected young and old mice to aerobic exercise and performed single cell transcriptome analysis of hematopoietic progenitors in the bone marrow of these animals.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

15 Samples

Download data: RDS

(Submitter supplied) To gain insight into the mechanisms by which exercise affects circulating immune cells, we subjected young and old mice to aerobic exercise and performed single cell transcriptome analysis of circulating immune cells from these animals.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

16 Samples

Download data: RDS