GEO DataSets

(Submitter supplied) Pyrococcus yayanosii CH1 is the first and only obligate piezophilic hyperthermophilic microorganism discovered so far, that extends the physical and chemical limits of life on Earth and strengthens the idea of the existence of a hyperthermophilic biosphere in the depth of our planet. It was isolated from the Ashadze hydrothermal vent at 4,100 m depth. Multi-omics analyses where performed in order to study the mechanisms implemented by the cell to face high hydrostatic pressure variations. more...

Organism:

Pyrococcus yayanosii; Pyrococcus; Pyrococcus furiosus

Type:

Expression profiling by array

Platform:

GPL20888

15 Samples

Download data: TXT

(Submitter supplied) We have identified differentially expressed genes according to hydrostatic pressure growth conditions in Desulfovibrio hydrothermalis. The transcriptomic datasets report the molecular mechanisms which could be involved in such adaptation and give information for the piezophile sulfate-reducing bacteria research communities. The data obtained pointed out a gradual response of D. hydrothermalis to an increase of hydrostatic pressure, with a threshold above 10 MPa and the involvement of a quite limited number of genes and/or pathways involved in the adaptation to hydrostatic pressure.

Organism:

Maridesulfovibrio hydrothermalis AM13 = DSM 14728

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18400

6 Samples

Download data: BEDGRAPH

(Submitter supplied) We have identified differentially expressed genes according to hydrostatic pressure growth conditions in Desulfovibrio piezophilus. The transcriptomic datasets report the molecular mechanisms which could be involved in such adaptation and give information for the piezophile sulfate-reducing bacteria research communities. The data obtained pointed out different responses of D. piezophilus to an increase of hydrostatic pressure.

Organism:

Pseudodesulfovibrio piezophilus C1TLV30

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18763

6 Samples

Download data: BEDGRAPH

(Submitter supplied) The remarkable survival of the hyperthermophilic archaeon Pyrococcus furiosus to ionizing radiation was previously demonstrated. Using a time course study and whole-genome microarray analyses of mRNA transcript levels, the genes and regulatory pathways involved in the repair of lesions produced by ionizing irradiation (oxidative damage and DNA strand breaks) in P. furiosus were investigated. Data analyses showed that radA, encoding the archaeal homolog of the RecA/Rad51 recombinase, was moderately up regulated by irradiation and that a putative DNA-repair gene cluster was specifically induced by exposure to ionizing radiation. more...

Organism:

Pyrococcus furiosus

Type:

Expression profiling by array

Dataset:

GDS2469

Platform:

GPL3926

21 Samples

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Analysis of Pyrococcus furiosus cells exposed to 2.5 kGy of gamma radiation and then incubated at 90º C for up to 4 hours. At this dose, P. furiosus exhibits 75% survival. Results provide insight into the pathways involved in the repair of lesions produced by ionizing irradiation in P. furiosus.

Organism:

Pyrococcus furiosus

Type:

Expression profiling by array, log10 ratio, 2 agent, 6 time sets

Platform:

GPL3926

Series:

GSE5919

21 Samples

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(Submitter supplied) Insights into the Metabolism of Elemental Sulfur by the Hyperthermophilic Archaeon Pyrococcus furiosus: Characterization of a Coenzyme A-Dependent NAD(P)H Sulfur Oxidoreductase The hyperthermophilic archaeon Pyrococcus furiosus, uses carbohydrates as a carbon source and produces acetate, CO2 and H2 as end products. When S° is added to a growing culture, within 10 min the rate of H2 production rapidly decreases and H2S is detected. more...

Organism:

Pyrococcus furiosus; Pyrococcus furiosus DSM 3638

Type:

Expression profiling by array

Platform:

GPL4688

18 Samples

Download data: TXT

(Submitter supplied) Glass slide, see protocol above. Protocol: Each of the known 2192 ORFs in P. furiosus were amplified individually by PCR. Primers were selected to produce full-length PCR products. In case of large ORFs >2 kb PCR primers were designed to yield a PCR product around 1 kb. Expected PCR products with high homology to more than one locus were replace by a 50-mer specific oligo. Oligos were purchased from MWG Biotech (High Point, NC) in a 96-well format. more...

Organism:

Pyrococcus furiosus DSM 3638

2 Series

27 Samples

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(Submitter supplied) Oxidative Stress Protection and the Repair Response To Hydrogen Peroxide in the Hyperthermophilic Archaeon Pyrococcus furiosus Pyrococcus furiosus is a shallow marine, anaerobic archaeon that grows optimally at 100°C. Addition of H2O2 (0.5 mM) to a growing culture resulted in cessation of growth with a 2 hour lag before normal growth resumed. Whole genome transcriptional profiling revealed that the main response occurs within 30 min of peroxide addition, with the up-regulation of 62 open reading frames (ORFs), 36 of which are part of 10 potential operons. more...

Organism:

Pyrococcus furiosus DSM 3638

Type:

Expression profiling by array

Platform:

GPL4688

9 Samples

Download data: TXT

(Submitter supplied) Transcriptome sequencing was carried out on an Illumina HiSeq platform to investigate the activation of CRISPR-Cas and DNA repair systems by Csa3a in Sulfolobus islandicus Rey15A. We compared the differently expressed genes in Sulfolobus islandicus Rey15A strain with csa3a overexpression vs. Sulfolobus islandicus Rey15A strain carrying an empty expression vector, cas1 deletion strain with csa3a overexpression vs. more...

Organism:

Sulfolobus islandicus REY15A

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL23498 GPL23497

12 Samples

Download data: XLS

(Submitter supplied) Pseudothermotoga elfii strain DSM9442 and P. elfii subsp. lettingae strain TMOT are hyperthermophilic bacteria. P. elfii is a moderate piezophile, isolated from an oil-producing well in Africa at a depth of more than 1600 m. P. lettingae is piezotolerant, isolated from a thermophilic bioreactor fed with methanol as the sole carbon and energy source. In this study, we analysed these bacteria at the genomic and transcriptomic levels. more...

Organism:

Pseudothermotoga lettingae TMO; Pseudothermotoga elfii DSM 9442 = NBRC 107921

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL33186 GPL33187

33 Samples

Download data: TXT