GEO DataSets

(Submitter supplied) A transcriptomic approach was used to evaluate potential interactions between prepartum body condition score (BCS) and feeding management in the weeks before calving on hepatic metabolism during the transition period. Thirty-two mid-lactation grazing dairy cows of mixed age and breed were randomly allocated to one of four treatment groups in a 2 × 2 factorial arrangement: two prepartum BCS categories [4.0 (thin, BCS4) and 5.0 (optimal, BCS5); based on a 10-point scale], and two levels of energy intake during the 3 wk preceding calving (75 and 125% of estimated requirements). more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL11648

48 Samples

Download data: GPR, XLSX

(Submitter supplied) Cows were selected from two groups of 12 cows enrolled in a large experiment to assess the effects of ad libitum or restricted intake of moderate-energy diets during the entire dry period on pre-partum metabolism and post-partum metabolism and performance. A corn silage-based diet (26% of diet dry matter) providing 1.59 Mcal/kg during the far-off dry period (first 5 wk of an 8-wk dry period) or providing 1.61 Mcal/kg during the close-up dry period (last 3 wk of the dry period) was fed for ad libitum or restricted intake. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2864

106 Samples

Download data

(Submitter supplied) The study investigated gene expression patterns in liver tissue to understand the biological functions of genes that are potentially involved in controlling / regulating feed efficiency in Nordic dairy cattle.

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19172

38 Samples

Download data: TXT

(Submitter supplied) In dairy cows, administration of high dosages of niacin (NA) was found to cause anti-lipolytic effects, which are mediated by the NA receptor hydroxyl-carboxylic acid receptor 2 (HCAR2) in white adipose tissue (WAT), and thereby to an altered hepatic lipid metabolism. However, almost no attention has been paid to possible direct effects of NA in cattle liver, despite showing that HCAR2 is expressed also in the liver of cattle and is even more abundant than in WAT. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL16500

18 Samples

Download data: CEL

(Submitter supplied) We evaluated liver transcriptome throughout gestation and early lactation of purebred and crossbred beef cows (Angus, Hereford and their F1 crossbreeds, CR), grazing high or low herbage allowances (HA) of native grasslands (4 and 2.5 kg DM/kg BW annual mean; n = 16) using an Agilent 4x44k bovine array. A total of 4,661 transcripts were affected by days (272 ≥ 2.5-fold difference, FDR ≤ 0.10) and 47 pathways were altered during winter gestation (-165 to -15 days relative to calving, d). more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL11648

64 Samples

Download data: TXT

(Submitter supplied) The ruminant liver has multiple roles in the dairy cow and many of these are crucial in nutrient supply during lactation. Reduced feed intake alters the expression of many genes and pathways in the liver, inducing a period of negative energy balance. Once-daily milking is a management strategy to reduce the effects of periods of negative energy balance so the objective of this study was to determine if once-daily milking altered hepatic gene transcription during a period of negative energy balance induce by caloric restriction. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL9712

45 Samples

Download data: TXT

(Submitter supplied) Background: The liver is central to most economically important metabolic processes in cattle. However, the changes in expression of genes that drive these processes remain incompletely characterised. RNA-seq is the new gold standard for whole transcriptome analysis but so far there are no reports of its application to analysis of differential gene expression in cattle liver. We used RNA-seq to study differences in expression profiles of hepatic genes and their associated pathways in individual cattle in either mild negative energy balance (MNEB) or severe negative energy balance (SNEB). more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL9323

11 Samples

Download data: TXT

(Submitter supplied) Negative energy balance (NEB) is an altered metabolic state in high yielding cows that occurs during the first few weeks postpartum when energy demands for lactation and maintenance exceed the energy supply from dietary intake. NEB can, in turn, lead to metabolic disorders and to reduced fertility. Alterations in the expression of more than 700 hepatic genes have previously been reported in a study of NEB in postpartum dairy cows. more...

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL15749

8 Samples

Download data: XLSX

(Submitter supplied) Beef cow adipose tissue transcriptome Evaluation of the naturally occurring transcriptome variation among beef cows with divergent gain.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL20768

24 Samples

Download data: CEL, CHP

(Submitter supplied) Periparturient cows rely on adipose tissue fatty acid reserves released by lipolysis to offset the negative energy balance induced by physiological changes related to parturition and the onset of lactation. However, lipolysis causes inflammation and structural remodeling in excess predisposes cows to disease. The objective of this study was to determine effects of the periparturient period on the transcriptomic profile of AT using NGS RNAseq

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23055

28 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL18534

21 Samples

Download data: CEL

(Submitter supplied) In high yielding dairy cows the liver undergoes extensive physiological and biochemical changes during the early postpartum period in an effort to re-establish metabolic homeostasis and to counteract the adverse effects of negative energy balance (NEB). These adaptations are likely to be mediated by significant alterations in hepatic gene expression. To gain new insights into these events an EB model was created using differential feeding and milking regimes to produce two groups of cows with either a mild (MNEB) (n=5) or severe NEB (SNEB) (n=6) status. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL18534

11 Samples

Download data: CEL

(Submitter supplied) Hepatic molecular adaptations underlying periparturient metabolic diseases such as ketosis in dairy cows are largely unknown. We used a simple model for induction of ketosis to examine liver gene expression profiles using a microarray consisting of 13,257 annotated cattle oligonucleotides. At 4 days post-partum, 7 cows classified as healthy after a physical examination were fed at 50% of intake at day 4 from day 5 to signs of ketosis or until 14 days post-partum. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2853

28 Samples

Download data

(Submitter supplied) The transition to lactation challenges dairy cows metabolically. Immune dysfunction and infectious disease risk is the hallmark of this transition period. Transcriptome data of PBMC shows differentially expressed pathways postpartum. Metabolically stressed cows show upregulation of innate immune pathways and inflammation.

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23055

12 Samples

Download data: DIFF, TXT

(Submitter supplied) Using an Agilent microarray platform, the present study examined changes in the transcriptome of bovine polymorphonuclear leukocytes (PMNL) due to prepartal dietary intake. Twelve Holstein cows were fed a high-straw, control diet (CON; NEL = 1.34 Mcal/kg) or overfed a moderate-energy diet (OVE; NEL = 1.62 Mcal/kg) during the dry period. Blood for PMNL isolation and metabolite analysis was collected at -14 and +7 d relative to parturition. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL11648

8 Samples

Download data: GPR

(Submitter supplied) Liver and mammary gland are among the most prominent organs during lactation in dairy cows. With the purpose to understand 1) the adaptation of liver and mammary to different levels of forage/concentrate ration (60:40 vs. 40:60) and 2) the crosstalk between the two organs during lactation, a transcriptome analysis was performed on liver and mammary tissues of 10 primiparous dairy cows in mid-lactation. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL11649

20 Samples

Download data: TXT

(Submitter supplied) Metabolic challenges experienced by dairy cows during the transition between pregnancy and lactation (also known as peripartum), are of considerable interest from a nutrigenomic perspective. The mobilization of large amounts of non-esterified fatty acids (NEFA) leads to an increase in NEFA uptake in the liver, the excess of which can cause hepatic accumulation of lipids and ultimately fatty liver. Interestingly, peripartum NEFA activate the peroxisome proliferator-activated receptor (PPAR), a transcriptional regulator with known nutrigenomic properties. more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21659

16 Samples

Download data: XLSX

(Submitter supplied) Increased energy demands to support lactation, coupled with lowered feed intake capacity results in negative energy balance (NEB) and is typically characterized by extensive mobilization of body energy reserves in the early postpartum dairy cow. The catabolism of stored lipid leads to an increase in the systemic concentrations of nonesterified fatty acids (NEFA) and -hydroxy butyrate (BHB). Oxidation of NEFA in the liver result in the increased production of reactive oxygen species and the onset of oxidative stress and can lead to disruption of normal metabolism and physiology. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL18534

10 Samples

Download data: CEL

(Submitter supplied) Our overall objective is to identify key differences in gene expression signaling pathways in the epithelial and intralobular stromal compartments during prepartum mammary remodeling and development in the dry cow.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

12 Samples

Download data: CEL

(Submitter supplied) The three different main cells types of the endometrium, have very different patterns of gene expression. The severity of NEB after calving is associated with changes in gene expression at time of breeding. Specific alterations in GEs are associated with activation of pro-inflammatory mechanisms. Concomitantly, changes in the expression of genes related to cell to cell interactions and maternal recognition of pregnancy takes place in ST. more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23295

24 Samples

Download data: SF