GEO DataSets

(Submitter supplied) Aging improves pancreatic β-cell function in mice. This is a surprising finding since aging is typically associated with functional decline. We performed single-cell RNA sequencing of β-cells from 3 and 26 month old mice to explore how changes in gene expression contribute to improved function with age. The old mice were healthy, had reduced blood glucose levels and increased β-cell mass, which correlated to their body weight. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

207 Samples

Download data: TXT

(Submitter supplied) Aging at the cellular level is driven by changes in gene activity and epigenetic state that are only partially understood. We performed a comprehensive epigenomic analysis of the pancreatic β cell, key player in glucose homeostasis and diabetes, in adolescent and very old mice. Globally, we observe a general methylation drift resulting in an overall more leveled methylome, suggesting that the maintenance of highly differential methylation patterns becomes compromised with advanced age. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing; Methylation profiling by high throughput sequencing

Platforms:

GPL13112 GPL17021

16 Samples

Download data: TAB, TXT

(Submitter supplied) Aging is associated with fundamental changes in pancreatic β-cell physiology; yet, the mechanisms that drive these age-related changes are poorly understood. We performed comprehensive proteomic profiling of pancreatic islets from adolescent and old mice. The analysis revealed striking differences in abundance of enzymes controlling glucose metabolism not reflected at the transcript level. We show that these changes in protein abundance are associated with increased activity of the amplifying pathway of insulin secretion. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

6 Samples

Download data: BIGWIG

(Submitter supplied) We used microarrays to investigate gene expression changes in the pancreas of RasGrf1 KO mice. These animals have a reduction in the number and size of the pancreatic islets which lead to lower levels of insulin and glucagon in their blood.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL339

6 Samples

Download data: CEL

(Submitter supplied) Here we present an RNAseq analysis of human bone samples, obtained from iliac crest needle biopsies, to yield the first in vivo interrogation of all genes and pathways that may be altered in bone with aging and E therapy in humans.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

58 Samples

Download data: CSV

(Submitter supplied) Maintenance of blood glucose homeostasis depends on appropriate pancreatic beta cell mass, which is established during early postnatal life through expansion of beta cell numbers. Postnatally, the proliferative capacity of beta cells declines rapidly but the cell extrinsic cues and intracellular signals that cause this decline remain unknown. Here we generated single-cell RNA-seq data of beta cells from multiple postnatal time points and ordered cells based on transcriptional similarity along a linear trajectory. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL21103 GPL17021

416 Samples

Download data: BIGWIG

(Submitter supplied) Relative beta cell deficit and increased beta cell apoptosis are hallmarks of type 2 diabetes (T2D). The Insulin/Insulin Growth Factor (Igf) signaling pathway is an established regulator of beta cell survival and is found downregulated in human T2D islets. The Insulin Receptor Substrate 2 (Irs2) plays a central role in the coordination of this pathway in beta cells. Thus, Irs2 knockout mice (Irs2 -/-) exhibit increased beta cell apoptosis that leads to a progressive decline of beta cell mass and hyperglycaemia. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5380

Platform:

GPL1261

8 Samples

Download data: CEL

Analysis of pancreatic islets from insulin receptor substrate 2 (Irs2) knockouts treated with anti-diabetic compound sodium tungstate for 21 days. Tungstate normalizes glucose tolerance in the Irs2 -/- animals. Results provide insight into the molecular effects of tungstate on pancreatic β-cells.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 agent, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE43620

8 Samples

Download data: CEL

(Submitter supplied) We use single-cell RNA-sequencing to investigate and identify immune cell types and transcriptomic changes with age

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

4 Samples

Download data: TXT

(Submitter supplied) As organisms age, cells accumulate genetic and epigenetic changes that eventually lead to impaired organ function or catastrophic failure such as cancer. Here we describe a single-cell transcriptome analysis of 2544 human pancreas cells from donors, spanning six decades of life. We find that islet cells from older donors have increased levels of disorder as measured both by noise in the transcriptome and by the number of cells which display inappropriate hormone expression, revealing a transcriptional instability associated with aging. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

2544 Samples

Download data: CSV