GEO DataSets

(Submitter supplied) We report the application of RNA-sequencing for high-throughput profiling of gene expression in hedgehog-responsive stromal cells in normal mouse prostate and mouse prostate tumors. By using the Gli1-GFP knock-in reporter mouse line, we isolated the subset of mouse prostate stromal cells undergoing hedgehog signaling to compare the transcriptomes between PB-MYC prostate tumor and normal prostate in mice at the age of about 45 weeks.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

6 Samples

Download data: XLS

(Submitter supplied) Inflammation has a causal role in many cancers. In prostate cancers, epidemiological data suggest a link between prostatitis and subsequent cancer development, but a proof for this concept in a tumor model has been lacking. A constitutively active version of the IkappaB kinase 2 (IKK2), the molecule activated by a plethora of inflammatory stimuli, was expressed specifically in the prostate epithelium. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4119

Platform:

GPL6246

6 Samples

Download data: CEL

Analysis of prostate epithelium (PE)-PTEN+/-IKK2ca/ca and PE-PTEN+/- lateral prostates. PE-PTEN+/-IKK2ca prostates exhibit loss of smooth muscle but fail to induce invasiveness. Results provide insight into the molecular mechanism of inflammation and tumor development in the transgenic prostates.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 genotype/variation sets

Platform:

GPL6246

Series:

GSE26410

6 Samples

Download data: CEL

(Submitter supplied) The T-box transcription factor TBX18 is essential to mesenchymal cell differentiation in several tissues and Tbx18 loss-of-function results in dramatic organ malformations and perinatal lethality. Here we demonstrate for the first time that Tbx18 is required for the normal development of periductal smooth muscle stromal cells in prostate, particularly in the anterior lobe, with a clear impact on prostate health in adult mice. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

4 Samples

Download data: TXT

(Submitter supplied) Androgen-signaling is essential for prostate development. However, how androgen action facilitates prostatic stem/progenitor initiated pubertal prostatic growth remains unclear. Here, we demonstrate that androgens regulate Shh-signaling to control the cellular niche in prostatic epithelial development. Selective deletion of androgen receptor (AR) in stromal Shh-responsive cells significantly impedes pubertal prostatic epithelial morphogenesis and growth. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

2 Samples

Download data: TXT

(Submitter supplied) In the intestine, Hedgehog (Hh) signalling orchestrates epithelial homeostasis in a bidirectional loop. Differentiated enterocytes secrete the ligand leading to active downstream signaling exclusively in the stroma. In turn, Hh-driven stromal factors contribute to the control of intestinal stem cell numbers and induce epithelial differentiation. To investigate the consequences of stromal Hh activation on the gene expression level, we performed microarray analysis on full-thickness colonic tissue from Col1a2CreER;Ptch1fl/fl mice (C57BL/6 background). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL17400

8 Samples

Download data: CEL

(Submitter supplied) Canonical Hedgehog (Hh) signaling regulates the expression of genes that are critical to the patterning and development of a variety of organ systems. In adult, both ligand-dependent and ligand-independent Hh pathway activation are known to promote tumorigenesis. Recent studies have shown that in tumors promoted by Hh ligand, activation occurs within the stromal microenvironment (Yauch et al., 2009). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4512

Platform:

GPL570

6 Samples

Download data: CEL, TXT

Analysis of CCD-18Co colon myofibroblasts stimulated with Sonic hedgehog homolog (SHH) for 72hr. Hedgehog (Hh)-driven, CCD-18Co proangiogenic signals drive tumor angiogenesis in vitro and in vivo. Results provide insight into the molecular mechanisms underlying Hh regulation of tumor angiogenesis.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL570

Series:

GSE29316

6 Samples

Download data: CEL

(Submitter supplied) In bladder, loss of mammalian Sonic Hedgehog (Shh) accompanies progression to invasive urothelial carcinoma, but the molecular mechanisms underlying this cancer-initiating event are poorly defined. Here, we show that loss of Shh results from hypermethylation of the CpG shore of the Shh gene, and that inhibition of DNA methylation increases Shh expression to halt the initiation of murine urothelial carcinoma at the early stage of progression. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

20 Samples

Download data: XLSX

(Submitter supplied) We performed gene expression profiling of laser capture microdissected normal non-neoplastic prostate (cystoprostatectomies) epithelial tissue and compared it to non-transformed and neoplastic low and high grade prostate epithelial tissue from radical prostatectomies, each with its immediately surrounding stroma.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

188 Samples

Download data: CEL

(Submitter supplied) We sorted lineage negative hematopoietic cells from mice previously transplanted with WT or Gli1 knockout bone marrow in both control and fibrosis settings. Cells were via magnetic depletion and facs sorting and performed scRNAseq on them. We identified 13 hematopoetic cells clusters across all lineages and one non-hematopoietic stromal cell cluster

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

8 Samples

Download data: MTX, TSV

(Submitter supplied) We sought to determine whether molecular alterations in tumor stroma influence prostate cancer progression and metastatic potential. To accomplish this, we compared mesenchymal cells from four genetically engineered mouse models (GEMMs) of prostate cancer representing different stages of the disease to their wild-type (WT) counterparts by single-cell RNA sequencing (scRNA-seq) and, ultimately, to human tumors with comparable genotypes. more...

Organism:

Mus musculus

Type:

Other

Platform:

GPL24247

2 Samples

Download data: H5, TIFF

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens; Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL24676 GPL24247

48 Samples

Download data: H5, TIFF

(Submitter supplied) We sought to determine whether molecular alterations in tumor stroma influence prostate cancer progression and metastatic potential. To accomplish this, we compared mesenchymal cells from four genetically engineered mouse models (GEMMs) of prostate cancer representing different stages of the disease to their wild-type (WT) counterparts by single-cell RNA sequencing (scRNA-seq) and, ultimately, to human tumors with comparable genotypes. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

17 Samples

Download data: H5AD

(Submitter supplied) We sought to determine whether molecular alterations in tumor stroma influence prostate cancer progression and metastatic potential. To accomplish this, we compared mesenchymal cells from four genetically engineered mouse models (GEMMs) of prostate cancer representing different stages of the disease to their wild-type (WT) counterparts by single-cell RNA sequencing (scRNA-seq) and, ultimately, to human tumors with comparable genotypes. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

29 Samples

Download data: H5AD

(Submitter supplied) The overall goal of this study was to compare gene expression profiles in MOAB-overexpressing PrSC cells and control cells, also to compare gene expression profiles in MAOB-knockdown and control cells.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21290

5 Samples

Download data: TXT

(Submitter supplied) Mice grafted with Myc-Cap tumor were treated (3 weeks) with a pro-apoptotic peptide D-CAN targeting adipose stromal cells or PBS (control).

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21626

4 Samples

Download data: H5, MTX, TSV