GEO DataSets

(Submitter supplied) Purpose: Monitor modulation of aneuploidy in Leishmania donovani during adaptation to different in vitro and in vivo environments, and its impact on gene expression Importance: Aneuploidy is usually detrimental in multicellular organisms, but in several micro-organisms (fungi being the best-studied) it can be tolerated and even beneficial. Leishmania – a protozoan parasite killing more than 30,000 persons each year – is emerging as a new model for aneuploidy studies: unexpectedly high levels of aneuploidy are found in clinical isolates. more...

Organism:

Leishmania donovani

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23261

11 Samples

Download data: TXT

(Submitter supplied) Genome instability has been recognized as a driver in Leishmania fitness gain in response to environmental change or chemotherapy. How genome instability generates beneficial phenotypes despite potential deleterious gene dosage effects is unknown. Here we address this important open question applying experimental evolution and integrative systems approaches on parasites adapting to in vitro culture. more...

Organism:

Leishmania donovani

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29657

15 Samples

Download data: TXT

(Submitter supplied) Leishmania infantum (Kinetoplastida:Trypanosomatidae) is the etiological agent of zoonotic visceral leishmaniasis in the Mediterranean basin. The motile promastigote stage infects the hematophagous sand fly vector host and amastigotes survives and multiplies within phagocytes of the mammalian host. Promastigotes are routinely cultured in liquid undefined media and are considered to mimic the environment within the sand fly gut. more...

Organism:

Leishmania infantum

Type:

Expression profiling by array

Platform:

GPL6781

1 Sample

Download data: TXT

(Submitter supplied) The aim of the experiment is to detect any gene expression modulation in an antimony resistant L.infantum Sb2000.1 strain compared to an antimony sensitive L.infantum WT strain. We want to identify the modulated genes associated with an antimony resistant phenotype. The Sb2000.1 resistant strain used is about 1000 times more resistant to SbIII than the WT sensitive strain. The full-genome DNA microarrays includes one 70-oligonucleotide probe for each gene of L. more...

Organism:

Leishmania infantum

Type:

Expression profiling by array

Platform:

GPL8804

5 Samples

Download data: GPR

(Submitter supplied) CGH analysis of the drug resistant strain L. major MTX60.4 vs the wild-type strain LV39 The full-genome DNA microarrays includes one 70-oligonucleotides probe for each gene of L.major LV39 Keywords: drug resistance

Organism:

Leishmania major; Leishmania infantum

Type:

Genome variation profiling by array

Platform:

GPL6855

3 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Leishmania infantum; Leishmania major

Type:

Expression profiling by array

Platform:

GPL6855

8 Samples

Download data: GPR

(Submitter supplied) Transcriptional analysis of the drug resistant strain L. major MTX60.4 compared to the wild-type strain LV39 The full-genome DNA microarrays includes one 70-oligonucleotides probe for each gene of L.major LV39 Keywords: drug resistance

Organism:

Leishmania infantum; Leishmania major

Type:

Expression profiling by array

Platform:

GPL6855

4 Samples

Download data: GPR

(Submitter supplied) Transcriptional analysis of the drug resistant strain L. infantum MTX20.5 compared to the wild-type strain The full-genome DNA microarrays includes one 70-oligonucleotides probe for each gene of L. infantum Keywords: drug resistance

Organism:

Leishmania infantum; Leishmania major

Type:

Expression profiling by array

Platform:

GPL6855

4 Samples

Download data: GPR

(Submitter supplied) Transcriptional analysis of L. infantum promastigote compared to L. infantum intracellular amastigote and transcriptional analysis of L. infantum promastigote compared to L. infantum axenic amastigote. The full-genome DNA microarrays includes one 70-oligonucleotides probe for each gene of L.infantum. Keywords: stage and culture condition

Organism:

Leishmania major; Leishmania infantum

Type:

Expression profiling by array

Platform:

GPL6855

10 Samples

Download data: GPR

(Submitter supplied) In this study, we examined the transcriptome of Leishmania donovani promastigotes and axenic amastigotes to identify differentially regulated mRNAs utilizing the serial analysis of gene expression Keywords: stage differentiation; axenic amastigotes

Organism:

Leishmania donovani

Type:

Expression profiling by SAGE

Platform:

GPL3252

2 Samples

Download data

(Submitter supplied) Histone acetylations are known to impact gene transcription. Here, we have attempted to examine the role of Leishmania donovani histone acetyltransferases HAT4 and HAT2 in regulating global gene expression. The transcriptome of HAT4-null promastigotes (Samples 2A, 2B) and HAT2-heterozygous knockout promastigotes (Samples 3A, 3B) have been compared with the transcriptome of wild-type Leishmania promastigotes (Samples 1A, 1B) and fold change in gene expression with respect to the control wild-type has been determined.

Organism:

Leishmania donovani

Type:

Expression profiling by array

Platform:

GPL18125

6 Samples

Download data: TXT, XLS

(Submitter supplied) Leishmania (L) are intracellular protozoan parasites which are able to survive and replicate within the harsh and potentially hostile phago-lysosomal environment of mammalian mononuclear phagocytes. A complex interplay then takes place between the macrophage (MΦ) striving to eliminate the pathogen and the parasite struggling for its own survival. To investigate, at the transcriptional level, this host-parasite conflict in the context of monocyte-derived human MΦs (MDM) infection by L. more...

Organism:

Leishmania major; Homo sapiens

Type:

Expression profiling by SAGE

Platforms:

GPL6471 GPL4

3 Samples

Download data

(Submitter supplied) We used next generation sequencing to examine global changes in gene expression in the spleen and splenic macrophages in a hamster model of progressive visceral leishmaniasis. We used RNA sequencing coupled with de novo transcriptome assembly, because the Syrian hamster does not have a fully sequenced and annotated reference genome. Libraries for deep sequencing were constructed from poly-A RNA isolated from the spleens or splenic macrophages of uninfected and 28-day L. more...

Organism:

Mesocricetus auratus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22776

16 Samples

Download data: FA, XLSX

(Submitter supplied) Aneuploidy variation can widely affect the expression of the whole genome, which is detrimental to organisms.Long noncoding RNAs (lncRNAs) are defined as RNAs that are longer than 200 nucleotides and lack a significant open reading frame.Although only a few lncRNAs have been well described, and most of them are still functionally unknown, there is sufficient evidence that lncRNAs have important cellular functions.We provide a comprehensive description of the characteristics and expression profiles of lncRNAs in aneuploid Drosophila. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25244

15 Samples

Download data: TXT