GEO DataSets

(Submitter supplied) In immunosurveillance, bone-derived immune cells infiltrate the tumor and secrete inflammatory cytokines to destroy cancer cells. However, cancer cells have evolved mechanisms to usurp inflammatory cytokines to promote tumor progression. In particular, the inflammatory cytokine, interleukin-1 (IL-1), is elevated in prostate cancer (PCa) patient tissue and serum and promotes PCa bone metastasis. IL-1 also represses androgen receptor (AR) accumulation and activity in PCa cells, yet the cells remain viable; suggesting that IL-1 may also contribute to AR-targeted therapy resistance. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

18 Samples

Download data: TAB

(Submitter supplied) AR+ PCa cell lines show conserved activation of canonical IL-1 intracellular signaling cascades, including repression of androgen receptor mRNA levels, in response to acute IL-1 treatment and show conserved loss of sensitivity to prolonged (chronic) IL-1 intracelluar signaling, including the restoration of the androgen receptor. Thus, chronic IL-1 exposure selects for cells that have a growth advantage against cytotoxic/static inflammation, including restoration of constitutive AR expression.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

15 Samples

Download data: TSV

(Submitter supplied) Chronic IL-1 treatment selects for cells that are less sensitive to cytotoxicity associated with serum starvation, loss of AR expression, and inflammatory cytokine signaling.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

9 Samples

Download data: TAB

(Submitter supplied) Background: Breast (BCa) and prostate (PCa) cancers are hormone receptor (HR)-driven cancers. Estrogen receptor alpha (ERa) is overexpressed in 70% of diagnosed BCa patients and androgen receptor (AR) is overexpressed in 80-90% of diagnosed PCa patients. Thus, BCa and PCa patients are given therapy that reduces hormone levels or directly blocks HR activity; but most patients eventually develop treatment resistance. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

12 Samples

Download data: TAB

(Submitter supplied) MicroRNAs (miRNAs) regulate a wide range of cellular signaling pathways and biological processes in both physiological and pathological states such as cancer. We have previously identified miR-135b as a direct regulator of androgen receptor (AR) protein level in prostate cancer (PCa). We wanted to further explore the relationship of miR-135b to hormonal receptors, particularly estrogen receptor α (ERα). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS6100

Platform:

GPL10558

12 Samples

Download data: TXT

Analysis of LNCaP prostate cancer (PCa) cells overexpressing miRNA-135b for up to 36 hours. LNCaP cells express the androgen receptor (AR). MiRNA-135b overexpression in AR+ PCa cells results in slower growth compared to AR knockdown. Results provide insight into the basis of this slower growth.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 protocol, 3 time sets

Platform:

GPL10558

Series:

GSE57820

12 Samples

Download data

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by array

Platforms:

GPL570 GPL9052

6 Samples

Download data: BED, CEL

(Submitter supplied) We report the high-throughput profiling of AR binding in prostate cancer cells.

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9052

2 Samples

Download data: BED

(Submitter supplied) Microarray experiments were performed for VCaP and VCS2 cells with and without androgen stimulation

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

4 Samples

Download data: CEL

(Submitter supplied) We compared 22 primary Pca (hormone-dependent) versus 29 metastatic Pca (CRPC). The expression of genes related to cell cycle, proliferation, DNA synthesis, and androgen metablism are significantly increased in CRPC group. The expression of AR-stimulated genes were partially reactivated. TMPRSS2-ERG fusion status was determined for the samples by PCR. The expression of ERG was highly increased in fusion positive versus negative.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL96

55 Samples

Download data: CEL

(Submitter supplied) Prostate epithelial cells depend on androgens for survival and function. In early prostate cancer, besides survival, androgens also regulated tumor growth, which is exploited by androgen ablation/ blockade therapies in metastatic disease. The aim of the present study was to characterize the role of the androgen receptor pathway in prostate cancer progression and to identify potential disease markers. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10367

21 Samples

Download data: TIFF, TXT

(Submitter supplied) Analysis of C4-2 Prostate cancer cell line after 72 hours of knockdown. CHKA is overexpressed in a number of solid tumours, including prostate cancer. Results provide insight into the molecular mechanisms of CHKA in prostate carcinogenesis.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

12 Samples

Download data: TXT

(Submitter supplied) Due to the urgent need of new targeting strategies in PCa, AR interacting proteins should be considered. In this study we aimed to test the effect of a long-term knockdown of NCOA1, an AR coactivator, in PCa progression and metastatogenesis and whether NCOA1 could be used as a possible therapeutic target. To test the consequences of NCOA1 knockdown on proliferation, we performed by 3H thymidine incorporation assays revealing a strong reduction in castration resistant MDA PCa 2b and androgen-dependent LNCaP cells, without affecting AR negative PC3 cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL18412

12 Samples

Download data: CEL

(Submitter supplied) Androgen receptor (AR) plays an essential role in normal prostate development and prostate cancer (PCa) progression. To understand the role of AR at the single-cell level, we performed single-cell transcriptome analysis on PCa cells stimulated with androgen and antiandrogen to reconstruct the dynamic and direct AR transcriptional network. Our work reveals that androgen stimulates the ER and Golgi stress response , promoting secreting protein trafficking, and inhibiting cell apoptosis. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL16791

3 Samples

Download data: BED, BW

(Submitter supplied) Androgen receptor (AR) plays key roles both in development of normal prostate gland and at all stages of prostate cancer. Due to the diversity of cell response to androgen stimulation, it’s necessary to investigate AR regulatory network at single cell level to fully understand how AR regulates transcription in prostate cancer cells. Here, we performed anti-androgen drug treated single cell RNA-seq profiling and transcriptome analysis on prostate cancer cell lines following 5α-dihydrotestosterone (DHT) stimulation. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

136 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

472 Samples

Download data

(Submitter supplied) Androgen receptor (AR) plays key roles both in development of normal prostate gland and at all stages of prostate cancer. Due to the diversity of cell response to androgen stimulation, it’s necessary to investigate AR regulatory network at single cell level to fully understand how AR regulates transcription in prostate cancer cells. Here, we performed normal growing and time-series 5α-dihydrotestosterone (DHT) stimulated single cell RNA-seq profiling and transcriptome analysis on prostate cancer cell lines. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

192 Samples

Download data: TXT

(Submitter supplied) Androgen receptor (AR) plays key roles both in development of normal prostate gland and at all stages of prostate cancer. Due to the diversity of cell response to androgen stimulation, it’s necessary to investigate AR regulatory network at single cell level to fully understand how AR regulates transcription in prostate cancer cells. Here, we performed time-series and anti-androgen drug treated single cell RNA-seq profiling and transcriptome analysis on prostate cancer cell lines following 5α-dihydrotestosterone (DHT) stimulation. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

144 Samples

Download data: TXT

(Submitter supplied) We previously encountered regulatory processes where dihydrotestosterone (DHT) exerted its inhibitory effect on parathyroid hormone-related protein (PTHrP) gene repression through the estrogen receptor (ER)α, but not the androgen receptor (AR) in breast cancer MCF-7 cells. Here, we investigated whether such an aberrant ligand-nuclear receptor (NR) interaction is present in prostate cancer LNCaP cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17077

8 Samples

Download data: TXT

(Submitter supplied) RNA-seq data were obtained from hTERT immortalized human prostate transit amplifying EP156T cells (+/- 10 nM R1881 for 48 hrs), progeny tumorigenic EPT3-M1 cells recovered from mouse metastatic tumor (+/- 10 nM R1881 for 48 hrs) and the prostate cancer cell lines LNCaP (+/- 10 nM R1881 for 48 hrs), VCaP (+/- 1 nM R1881 for 24 hrs) and 22Rv1 (+/- 1 nM R1881 for 24 hrs) (obtained from the American Type Culture Collection). more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

10 Samples

Download data: FPKM_TRACKING