GEO DataSets

(Submitter supplied) Maturation of lung cell types occurs during late gestation to ensure lung function and optimal gas exchange at birth. The pulmonary lymphatic endothelium is an understudied cell type and is essential for immune regulation and interstitial fluid removal. Here we provide the first characterization of the pulmonary lymphatic endothelium during late gestation prior to birth. We used microarrays to detail the global transcriptional programme underlying maturation of pulmonary lymphatic endothelium and identified 1,281 genes with significant changes in gene expression over time. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL17791

9 Samples

Download data: CEL

(Submitter supplied) Amniotic fluid was colelcted during midtrimester from 30 women and at term gestation from 68 women. Cell free RNA was profiled by HTA 2.0 arrays to study the effect of gestational age and other relevant convariates on gene expression and splicing during normal pregnancy.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17586

98 Samples

Download data: CEL

(Submitter supplied) In this study, time dependent genome wide lung mRNA profiling changes were assessed using C57BL/6J and A/J mice. Through comprehensive bioinformatics and functional genomics analyses, we identified both temporal and strain dependent gene expression patterns, systemically mapped key regulators, bioprocesses, and transcriptional networks controlling lung maturation, providing the basis for new therapeutic strategies to enhance lung function in preterm infants. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

37 Samples

Download data: CEL

(Submitter supplied) Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infection of 3rd trimester pregnant pigs can result in transmission of the virus to the fetus and ultimately death in utero or postnatally. Little is known about the immune response to infection at the maternal-fetal interface and in the fetus itself, or the molecular events behind virus transmission and disease progression in the fetus. To investigate these processes, RNA-sequencing of two tissues, uterine endothelium adjacent to the umbilical attachment site and fetal thymus, was performed 21 days post challenge on four groups of fetuses selected from a large PRRSV challenge experiment of pregnant gilts.

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11429

96 Samples

Download data: TXT

(Submitter supplied) To further knowledge of piglet maturity, we have developed a microarray analysis to describe biological processes and to find candidate genes for key roles in piglet maturity. The objective was to identify which genes and biological processes are specifically involved in the difference between two extreme breeds: Large White (LW) and Meishan (MS). The LW breed is a selected breed known to show an increased rate of mortality at birth, while the MS breed presents more robust piglets at birth. more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL19893

63 Samples

Download data: TXT

(Submitter supplied) In order to shed light on human kidney development, we have used single-cell transcriptomics to characterize gene expression in different cell population, and to study individual cell dynamics and lineage trajectories during development

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

5 Samples

Download data: TXT

(Submitter supplied) We conducted the genome-wide mRNA expression profiling in parallel with miRNA expression profiling using RNA samples extracted from rat incisor enamel organs, so as to refine the number of predicted gene targets for miRNA regulators.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL6101

8 Samples

Download data: TXT

(Submitter supplied) Fetal lung development is a complex biological process, which involves temporal and spatial regulations of many genes. To understand molecular mechanisms of this process, we investigated gene expression profiling of lungs at gestational day 18, 19, 20, 21, new born, and adult rats using in-house rat DNA microarray containing 6,000 known genes and 4,000 ESTs. 1,512 genes passed SAM test and 583 genes (402 known genes and 181 ESTs) had a 2-fold change at least at one time point. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1699

54 Samples

Download data

(Submitter supplied) Amniotic fluid is critical for the development of foetus, which provides the appropriate conditions for foetus growth. Besides its presumed traditional roles of providing movement place and protecting from crushing, amniotic fluid is considered to have more important roles in the nervous development of foetus respecting nervous related compositions and close contact with the skin of foetus. Peripheral nervous sensory system is the feeling basis for the body with neuritis anchoring on the surface of skin. more...

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array

Platform:

GPL20632

3 Samples

Download data: GPR

(Submitter supplied) Purpose: Exploring mechanisms defining the unique nature of vascular development and differentiation in the brain . Methods: High-resolution gene expression profiles of embryonic endothelial cells (EC) using translating ribosome affinity purification (TRAP) and single cell RNA-sequencing. RNA-sequenzing of transcription factor infected HUVEC. Results: Comparisons of different organ-, temporal- or Ctnnb1 knock out-specific vascular translatomes revealed extensive molecular changes during the unique development of the brain endothelium. more...

Organism:

Homo sapiens; Mus musculus

Type:

Expression profiling by high throughput sequencing; Other

Platforms:

GPL11154 GPL13112

175 Samples

Download data: TXT

(Submitter supplied) Blood was donated by a healthy volunteer with no known illness or malignancies. Mononuclear cells were separated from the blood by density gradient and CD14+ cells were isolated using anti-CD14 IgG-conjugated magnetic beads and magnetic separation (Miltenyi Biotec). Cells were cultured for 7 days prior to treatment for 96 hours with one of three TLR4 ligands: LPS, HMGB1 or Nab-Paclitaxel. Total RNA was extracted and was analyzed via SYBR Green RT-qPCR. more...

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL21496

12 Samples

Download data: TXT

(Submitter supplied) Blood was donated by four healthy volunteers with no known illness or malignancies. Mononuclear cells were separated from the blood by density gradient and CD14+ cells were isolated using anti-CD14 IgG-conjugated magnetic beads and magnetic separation (Miltenyi Biotec). We used an in-house array to analyze the gene expression of a total of 270 targets including 67 cytokines, 57 cytokine receptors, 18 chemokines, 11 chemokines receptors, 10 known TLR4 downstream targets, 64 other inflammation-related proteins, 12 genes expressed primarily in lymphatic endothelial cells, and 32 endothelial cell associated proteins. more...

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL21190

12 Samples

Download data: TXT

(Submitter supplied) The placenta is a dynamic organ which undergoes extensive remodeling throughout pregnancy to support, protect and nourish the developing fetus. Despite the importance of the placenta, very little is known about its gene expression beyond very early pregnancy and post-partum. Therefore, we utilized RNA-sequencing to characterize the transcriptome from the fetal (chorioallantois) and maternal (endometrium) components of the placenta from mares throughout gestation (4, 6, 10, 11 m). more...

Organism:

Equus caballus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24409

15 Samples

Download data: XLSX

(Submitter supplied) Mechanical forces are essential for normal fetal lung development. However, the cellular and molecular mechanisms regulating this process remain largely unknown. In the present study, we used oligonucleotide microarray technology to investigate gene expression profile in cultured E19 rat fetal lung type II epithelial cells exposed to a level of mechanical strain similar to that observed in utero. Significance Analysis of Microarrays (SAM) identified 92 genes differentially expressed by strain. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS2225

Platform:

GPL341

6 Samples

Download data: CEL, EXP

Analysis of embryonic day 19 fetal lung type II epithelial cells exposed to mechanical strain for 16 hours. Mechanical forces are essential for normal fetal lung development. Results provide insight into the mechanisms regulating lung development in response to mechanical forces.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 stress sets

Platform:

GPL341

Series:

GSE3541

6 Samples

Download data: CEL, EXP

(Submitter supplied) Normal development in the brain requires temporal changes in gene expression. A better understanding of the genomics of the fetal brain during late gestation can help to improve our understanding of the molecular events that enable the newborn to survive extra-uterine life. The purpose of the present study is to model changes and coherence of gene expression in cerebral cortex, brainstem, hippocampus, and hypothalamus throughout the second half of gestation. more...

Organism:

Ovis aries

Type:

Expression profiling by array

Platform:

GPL14112

95 Samples

Download data: TXT

(Submitter supplied) Purpose: The goal of this study is to provide a detailed transcriptome expression profile of the mouse cornea and evaluate changes in gene expression during mouse corneal development. Methods: RNA-sequencing is used to profile the mouse transcriptome in the pNC isolated at E10.5 and corneas isolated at E14.5 and E16.5. Samples generated in triplicate for sequencing by Illumina Hiseq 4000. Clean reads are mapped to reference gene and GRCm38 by Bowtie2 and HISAT. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

9 Samples

Download data: TXT

(Submitter supplied) Our strategy was to manipulate mTOR signaling in vivo, then characterize the transcriptome and translating mRNA in liver tissue. In adult rats, we used the non-proliferative growth model of refeeding after a period of fasting, and the proliferative model of liver regeneration following partial hepatectomy. We also studied livers from pre-term fetal rats (embryonic day 19-20) in which fetal hepatocytes are asynchronously proliferating. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL6247

40 Samples

Download data: CEL

(Submitter supplied) Transcriptional profiling of human umbilical vein endothelial cells, treated with MASP-1, thrombin, LPS, histamine, TNFalpha, MASP-1+SB203580 (p38-MAPK inhibitor) or MASP-1+Bay-117082 (NFKB inhibitor). Goal was to determine the effect of MASP-1 on HUVECs, to compare this with the effect of other endothelial cell activators and to determine the MASP-1 induced signalling pathways.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL14550

13 Samples

Download data: TXT

(Submitter supplied) IFNs are highly pleiotropic cytokines also endowed with marked anti-angiogenic activity. In this study, the mRNA expression profiles of endothelial cells (EC) exposed in vitro to IFN-alpha, IFN-beta, or IFN-gamma were determined. We found that in HUVEC as well as in other EC types 175 genes were upregulated (>2-fold increase) by IFNs, including genes involved in the host response to RNA viruses, inflammation, and apoptosis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2516

Platform:

GPL96

23 Samples

Download data