GEO DataSets

(Submitter supplied) The spatial transcriptome data has allowed us to explore the differential gene expression among the oral-aboral domains and provided new insights into the apical organ signalling pathways for future mechanistic studies and genes associated with oral/aboral differentiation. This resource identifies numerous candidates for elucidating the functional role of the apical organ and to address the evolution of the metazoan larval nervous system.

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29226

10 Samples

Download data: TXT

(Submitter supplied) Cnidarians are the only non-bilaterian group to evolve ciliated larvae with an apical sensory organ integrated with neurons, possibly homologous to the bilaterians. Within cnidarians, an apical organ with a ciliary tuft is identified only in sea anemones. Whether this apical tuft has evolved independently in anthozoans or alternatively originated in the eumetazoans and was lost independently in specific groups of cnidarians and bilaterians is uncertain.

Organism:

Aurelia aurita; Acropora millepora; Acropora tenuis; Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

4 related Platforms

18 Samples

Download data: FASTA, TXT

(Submitter supplied) Bilaterian animals have evolved complex sensory organs comprised of distinct cell types that function coordinately to sense the environment. Each sensory unit has a defined architecture built from component cell types, including sensory cells, non-sensory support cells, and dedicated sensory neurons. Whether this characteristic cellular composition is present in the sensory organs of non-bilaterian animals is unknown. more...

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29855

1 Sample

Download data: H5

(Submitter supplied) We performed single-cell transcriptome analysis (using MARS-seq) in the cnidarian Nematostella vectensis. Additionally we performed bulk ATAC-seq experiments on wild-type specimens, as well as ELAV::mOrange reporter transgenic lines (separating ELAV+ and ELAV- cell populations, representing neurons and non-neurons, respectively).

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL23029

8 Samples

Download data: BW, TXT, XLSX

(Submitter supplied) During early animal evolution, the emergence of axially-polarized segments was central to the diversification of complex bilaterian body plans. Nevertheless, precisely how and when segment polarity pathways arose remains obscure. Here we demonstrate the molecular basis for segment polarization in developing larvae of the pre-bilaterian sea anemone Nematostella vectensis. Utilizing spatial transcriptomics, we first constructed a 3-D gene expression atlas of developing larval segments. more...

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL23029 GPL29226

31 Samples

Download data: CSV, H5

(Submitter supplied) Transcription factor brachyury plays a key role during mesoderm development in vertebrates. In this study we study study the effect of this transcription factor in the purple sea urchin S. purpuratus.

Organism:

Strongylocentrotus purpuratus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL20965

10 Samples

Download data: BED, TSV

(Submitter supplied) Environmental contamination from heavy metals poses a global concern for the marine environment, as heavy metals are passed up the food chain and persist in the environment long after the pollution source is contained. Cnidarians, dating back to 700 million years ago, play an important role in shaping marine ecosystems, but environmental pollution profoundly affects their vitality. Among the cnidarians, the sea anemone Nematostella vectensis is an advantageous model for addressing questions in molecular ecology and toxicology as it tolerates extreme environments and its genome has been published. more...

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17090

16 Samples

Download data: TXT

(Submitter supplied) A diversity of life cycle stages processed into single-cell RNA-seq libraries in order to characterize cell type diversity and relationships

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL29226 GPL23029

16 Samples

Download data: CSV, GTF, MTX, TSV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL23029 GPL23802

8 Samples

Download data: MTX, TSV

(Submitter supplied) Isolated mesenteries from MHC::mCherry Adults were used to generate FACS-sorted RNA sequencing libraries in order to characterize gene use in Cnidarian smooth muscle. Transgenic mCherry expressing cells are compared to the non-expressing cells of the surrounding tissues, and up-regulated genes within the muscle cell population can be identified as important muscle-related gene products.

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23802

4 Samples

Download data: TXT

(Submitter supplied) Sub-adult tissue pieces were processed into single cell RNA sequencing libraries in order to catalog spatial information and cell type distribution

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23029

4 Samples

Download data: MTX, TSV

(Submitter supplied) We demonstrated that the presence of substrate is effectively required for transverse fission, that animal size does not affect fission rates, and that the position of physal pinching is fixed along the oral-aboral axis of the polyp. Gene expression analyses revealed a role for putative homeobox transcription factors, and components of TGFβ, Notch, and FGF signaling pathways in the transverse fission process.

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23029

6 Samples

Download data: TXT

(Submitter supplied) Single cell atlases of platyhelminth Müller’s larva and mollusc trochophore larva reveal homologous cell types and phylum specific novelties

Organism:

Crassostrea gigas; Prostheceraeus crozeri

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL24339 GPL33378

3 Samples

Download data: MTX, TSV