GEO DataSets

(Submitter supplied) In this work we investigated the molecular mechanisms that sustain the endothelial differentiation of murine embryonic stem cells (ES). When ES cells are co-cultured with the stromal PA6 cells in serum-free medium they differentiate mainly into neurons, thanks to the neural inducing activity exerted by the stroma. The addition of exogenous FGF2 allows also the differentiation of endothelial cells whereas, in presence of exogenous BMP4, ES cells differentiate exclusively into endothelium. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

16 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

40 Samples

Download data

(Submitter supplied) In this work we investigated the molecular mechanisms that sustain the endothelial differentiation of murine embryonic stem cells (ES). When ES cells are co-cultured with the stromal PA6 cells in serum-free medium they differentiate mainly into neurons, thanks to the neural inducing activity exerted by the stroma. The addition of exogenous FGF2 allows also the differentiation of endothelial cells whereas, in presence of exogenous BMP4, ES cells differentiate exclusively into endothelium. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

6 Samples

Download data: TXT

(Submitter supplied) In this work we investigated the molecular mechanisms that sustain the endothelial differentiation of murine embryonic stem cells (ES). When ES cells are co-cultured with the stromal PA6 cells in serum-free medium they differentiate mainly into neurons, thanks to the neural inducing activity exerted by the stroma. The addition of exogenous FGF2 allows also the differentiation of endothelial cells whereas, in presence of exogenous BMP4, ES cells differentiate exclusively into endothelium. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

18 Samples

Download data: TXT

(Submitter supplied) H1 hESC were exposed to BMP4 (10 ng/ml) in absence of FGF2 for 0, 3, 12, 24, 72, and 120 h under both physiological (4 % O2) and atmospheric (20 % O2) conditions to identify candidate genes and gene networks associated with specification from totipotent precursor cells to trophoblast. RNA from cells under both conditions was isolated at each time point. Approximately 32,000 probe sets (out of 40,391) provided a signal above background at one of the time points. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

12 Samples

Download data: TXT

(Submitter supplied) NANOG has emerged as a central gatekeeper of pluripotency. Here we show that as human embryonic stem (ES) cells exit the pluripotent state, NANOG can play a key role in determining lineage outcome. It has previously been reported that BMPs can induce differentiation of human ES cells into extraembryonic lineages. Here we report that FGF2 switches BMP4 induced differentiation outcome to mesendoderm, characterized by the uniform expression of T (brachyury) and other primitive streak markers. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL6602 GPL9999

50 Samples

Download data: PAIR

(Submitter supplied) Mouse embryonic fibroblasts stimulated with varying doses of FGF2 in conventional hES cell medium were analysed on whole-genome expression chips to reveal altered expression of genes encoding secreted proteins. Keywords: Growth factor stimulation experiment

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2421

Platform:

GPL4055

4 Samples

Download data: TXT

(Submitter supplied) Mouse embryonic fibroblasts stimulated with 40 ng/ml of FGF2 in conventional hES cell medium were analysed on whole-genome expression chips to reveal altered expression of genes encoding secreted proteins. Keywords: Growth factor stimulation experiment

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4865

2 Samples

Download data: TXT

(Submitter supplied) Mouse embryonic fibroblasts stimulated with 40 ng/ml of FGF2 in conventional hES cell medium were analysed on whole-genome expression chips to reveal altered expression of genes encoding secreted proteins. Keywords: Growth factor stimulation experiment

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4865

2 Samples

Download data: TXT

(Submitter supplied) Hybridisations were carried out to define a fibroblast-like cell type derived from H9 human embryonic stem cells. Keywords: cell type comparison

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2423

Platform:

GPL2700

4 Samples

Download data: TXT

(Submitter supplied) This series includes hES cell samples starved and re-stimulated with FGF2. Controls were FGF2-starved but not re-stimulated. Two independent experiments were carried out (one sample plus control each). Keywords: Growth factor stimulation experiment

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2422

Platform:

GPL2700

4 Samples

Download data: TXT

Analysis of embryonic fibroblast-like (EF) differentiated cells derived from H9 embryonic stem cells. Foreskin fibroblasts, and undifferentiated H9 and embryonic carcinoma cells also examined. Results provide insight into the differences between EF cells and fibroblasts at the molecular level.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 4 cell type sets

Platform:

GPL2700

Series:

GSE5416

4 Samples

Download data: TXT

Analysis of embryonic fibroblasts starved of fibroblast growth factor 2 (FGF2) for 2 days and subsequently restimulated with FGF2. FGF2 promotes the self-renewal of embryonic stem cells (ESCs) . Results provide insight into the molecular mechanisms underlying ESC self-renewal in response to FGF2.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 2 protocol sets

Platform:

GPL2700

Series:

GSE5415

4 Samples

Download data: TXT

Analysis of embryonic fibroblasts after treatment with fibroblast growth factor 2 (FGF2) at 4, 13, and 40 ng/ml for 24 hours. FGF2 promotes the self-renewal of embryonic stem cells (ESCs) . Results provide insight into the molecular mechanisms underlying ESC self-renewal in response to FGF2.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 4 dose sets

Platform:

GPL4055

Series:

GSE5437

4 Samples

Download data: TXT

(Submitter supplied) Purpose: Use scRNA Seq to find stromal subpopulations and define how these subpopulations contribute to salivary gland proacinar differentiation. Method: scRNA Seq on stromal enriched cells from E16 salivary organoids. Model closely related cells using PCA and UMAP regressions. Find genes that broadly and specifically define cell subpopulations. Results: The stromal fibroblasts have multiple subpopulations. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

2 Samples

Download data: CSV, MTX, TSV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array; Expression profiling by high throughput sequencing

4 related Platforms

15 Samples

Download data: CEL, MTX, TSV

(Submitter supplied) Purpose: Use scRNA Seq to find B10stromal subpopulations and define how these subpopulations contribute to salivary gland proacinar differentiation. Method: scRNA Seq on stromal enriched cells from E16 salivary glands. Model closely related cells using PCA and UMAP regressions. Find genes that broadly and specifically define cell subpopulations. Results: The epithelium and stromal fibroblasts have multiple subpopulations. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

1 Sample

Download data: CSV, MTX, TSV

(Submitter supplied) FGF2 signals BMP7 expression and TGFB/myofibroblast gene inhibition that promotes proacinar differentiation. We have characterized these stromal factors affecting the epithelium using a combination of scRNA Sequencing of whole tissue and transcriptional profiling of the organoid cell compartments.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL20775

6 Samples

Download data: CEL

(Submitter supplied) FGF2 signals BMP7 expression and TGFB/myofibroblast gene inhibition that promotes proacinar differentiation. We have characterized these stromal factors affecting the epithelium using a combination of scRNA Sequencing of whole tissue and transcriptional profiling of the organoid cell compartments.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL23038

6 Samples

Download data: CEL

(Submitter supplied) Bone morphogenetic protein (BMP) signaling is known to support differentiation of human embryonic stem cells (hESCs) into mesoderm and extraembryonic lineages, whereas other signaling pathways can largely influence this lineage specification. Here, we set out to reinvestigate the influence of ACTIVIN/NODAL and fibroblast growth factor (FGF) pathways on the lineage choices made by hESCs during BMP4-driven differentiation. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6883

24 Samples

Download data: TXT