GEO DataSets

Expression profiling of uteri from ovariectomized C57BL/6 animals at various time points up to 24 hours following treatment with estradiol (E2). E2 plays a critical role in regulating the growth, differentiation, and secretory function of the uterus.

Organism:

Mus musculus

Type:

Expression profiling by array, log10 ratio, 5 time sets

Platform:

GPL891

Series:

GSE4664

10 Samples

Download data: TIFF, TXT

(Submitter supplied) Using microarray technology, we compared the global expression pattern of uterine RNA from ovariectomized control mice to those of ovariectomized mice treated with estradiol for various intervals between 30 minutes and 24 hours. Keywords: estrogen, uterus, genomic, mouse

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2208

Platform:

GPL891

10 Samples

Download data: TIFF, TXT

(Submitter supplied) A proposed membrane-mediated mechanism of rapid non genomic response to estrogen has been the intense focus of recent research. Estren (Es), a synthetic steroid, is reported to act selectively through a rapid membrane-mediated pathway, rather than through the classical nuclear estrogen receptor (ER)-mediated pathway, to maintain bone density in ovaierectomized mice without uterotropic effects. To further evaluate the mechanism and physiological effects of Es we studied responses in adult ovariectomized mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS2077 GDS2078

Platform:

GPL891

22 Samples

Download data: TIFF, TXT

Analysis of uteri from adult ovariectomized estrogen receptor alpha (ERalpha) null mutants treated with estren, estradiol, or 19-nortestosterone for 2 or 24 hours. Estren is a synthetic steroid. Results provide insight into the dependence of gene responses induced by estren in the uterus on ERalpha.

Organism:

Mus musculus

Type:

Expression profiling by array, log10 ratio, 3 agent, 2 time sets

Platform:

GPL891

Series:

GSE4615

10 Samples

Download data: TIFF, TXT

Analysis of uteri from adult ovariectomized animals treated with estren, estradiol, dihydrotestosterone, or 19-nortestosterone for 2 or 24 hours. Estren is a synthetic steroid. Results provide insight into the molecular and physiological effects of estren on the uterus.

Organism:

Mus musculus

Type:

Expression profiling by array, log10 ratio, 4 agent, 2 time sets

Platform:

GPL891

Series:

GSE4615

12 Samples

Download data: TIFF, TXT

(Submitter supplied) Estrogens exert many important effects in bone, a tissue that contains both estrogen receptors alpha and beta (ERalpha and ERbeta). To compare the actions of these receptors, we generated U2OS human osteosarcoma cells stably expressing ERalpha or ERbeta, at levels comparable to those in osteoblasts, and we characterized their response to estradiol (E2) over time using Affymetrix GeneChip microarrays to determine the expression of approximately 12,000 genes, followed by quantitative PCR verification of the regulation of selected genes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS884

Platform:

GPL91

20 Samples

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Expression profiling of U2OS osteosarcoma cells after treatment with 17beta-estradiol (E2) for various lengths of time up to 48 hours. U2OS transfected with either estrogen receptor (ER) alpha or beta. Results identify signaling pathways in bone regulated by E2 through ERalpha and ERbeta.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 2 cell line, 5 time sets

Platform:

GPL91

Series:

GSE1153

20 Samples

Download data

(Submitter supplied) 17ß-Estradiol (E2) is well known to be associated with uterine cancer, endometriosis, and leiomyomas. Although insulin-like growth factor I (IGF-I) has been identified as a mediator of the uterotrophic effect of E2 in several studies, this mechanism is still not well understood. In the present study, identification of the genes modulated by a physiological dose of E2, in the uterus, has been done in ovariectomized mice using Affymetrix microarrays. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2638

Platform:

GPL81

6 Samples

Download data: CEL

Analysis of uteri from overiectomized animals at various lengths of time up to 24 hours following treatment with 17beta-estradiol (E2). Results provide insight into the molecular basis of the uterotrophic effect induced by a physiological dose of E2.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 6 time sets

Platform:

GPL81

Series:

GSE6219

6 Samples

Download data: CEL

(Submitter supplied) Ovariectomized WT, KIKO (DNA-binding deficient ERα) or αERKO female mice were injected (ip) with saline (vehicle), estradiol (E2; 250 ng), bisphenol A (BPA; 750 µg) or 2,2-bis(p-hydroxyphenyl)-1,1,1-trichloroethane (HPTE; 750 µg) and uteri were collected after 2 or 24 hours. Uterine profiles were compared and indicated the early (2 hour) responses to E2 were highly correlated to the BPA and HPTE profiles. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL4134 GPL7202

36 Samples

Download data: TIFF, TXT

(Submitter supplied) Estrogens stimulate hypertrophy and hyperplasia in the uterus and exert their activity through estrogen receptor α (ERα). A uterine epithelial ERα conditional knockout mouse model (Wnt7aCre+;Esr1f/f or cKO) demonstrated that ERα in the epithelial cells was dispensable for an early uterine proliferative response to 17β-estradiol (E2), but required for subsequent uterine biological responses. We compared the gene expression profile in the uterus after E2 treatment in the cKO samples with WT samples. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5461

Platform:

GPL4134

18 Samples

Download data: TXT

(Submitter supplied) WT and Ex3aERKO females were ovariectomized and injected with saline or estradiol. Uterine tissue was collected after 2 or 24 hours. RNA was analyzed by microarray to determine if the Ex3aERKO mice would lack the residual transcritpional resposnes seen in the previous aERKO model.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4134

18 Samples

Download data: TIFF, TXT

Analysis of uteri from ovariectomized adult females with uterine epithelial cell-specific ERα deletion following treatment with 17β-estradiol for 2 or 24 hrs. Results provide insight into cell specific actions of ERα in the female reproductive tract.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 2 genotype/variation, 2 time sets

Platform:

GPL4134

Series:

GSE53812

18 Samples

Download data: TXT

(Submitter supplied) The ovarian hormones estrogen and progesterone orchestrate the transcriptional programs required to direct functions of the uterus for initiation and maintenance of pregnancy. Estrogen, acting via estrogen receptor alpha (ERα), regulates gene expression by activating and repressing distinct genes involved in signaling pathways that regulate cellular and physiological responses including cell division, water influx, and immune cell recruitment. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21626

12 Samples

Download data: BW

(Submitter supplied) Females were ovariectomized and injected with sesame oil, estradiol in sesame oil, BPA in sesame oil or HPTE in sesame oil. Uterine tissue was collected after 2 or 24 hours. RNA was analyzed by microarray compare ealry and late responses to a potent and a weak estrogen agaonist.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4134

20 Samples

Download data: TIFF, TXT

(Submitter supplied) Females were ovariectomized and injected with saline estradiol or estriol. Uterine tissue was collected after 2 or 24 hours. RNA was analyzed by microarray compare ealry and late responses to a potent and a weak estrogen agaonist.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4134

15 Samples

Download data: TIFF, TXT

(Submitter supplied) Estrogen induce organ-specific cell proliferation and development in female reproductive organs, though the reproductive differentiation, sex maturation, implantation and lactation. However, the mechanism of organ-specific estrogen responsive genes is unknown. Thus, we examined early estrogen responsive genes in mouse uterus, vagina and mammary gland. Keywords: organ specificity

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL81

12 Samples

Download data: CEL, EXP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL11002 GPL9250

10 Samples

Download data: BED, BEDGRAPH

(Submitter supplied) To advance understanding of mechanisms leading to biological and transcriptional endpoints related to estrogen action in the mouse uterus, we have mapped ERα and RNA polymerase II binding sites using chromatin immunoprecipitation (ChIP) followed by sequencing of enriched chromatin fragments (ChIP-seq). In the absence of hormone, 5184 ERα binding sites were apparent in the vehicle treated ovariectomized uterine chromatin, while 17240 were seen one hour after estrogen (E2) treatment, indicating that some sites are occupied by unliganded ERα, and that ERα binding is increased by E2. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9250

5 Samples

Download data: BED, BEDGRAPH

(Submitter supplied) Pre-pubertal (21 days old) WT or ERa knockout we compared RNA from 21-day old WT and Ex3aERKO uteri that were treated with saline vehicle (V) estradiol (E2) or IGF1 for 2 hours or 24 hours

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL20775

30 Samples

Download data: CEL, CHP