GEO DataSets

Analysis of mRNA purified by mRNA-tagging from photoreceptors cells. These cells are engineered to express a FLAG-tagged poly(A)-binding protein (PABP) that allows separation of their mRNA from those of cells not expressing the tagged PABP. Results provide insight into the validity of this approach.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array, count, 2 genotype/variation, 2 tissue sets

Platform:

GPL72

Series:

GSE1790

14 Samples

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(Submitter supplied) The method of mRNA tagging was recently developed to isolate mRNA population from muscle tissues in Caenohabditis elegans. This method utilizes a FLAG-tagged poly(A)-binding protein (PABP), which can bind to poly(A)-tail of eukaryotic mRNA. Thus, the mRNA population from specific tissue, in which FLAG- tagged PABP is expressed, can be co-immunoprecipitated with FLAG-tagged PABP using FLAG-specific antibody. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Dataset:

GDS2479

Platform:

GPL72

14 Samples

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(Submitter supplied) Background: With its fully sequenced genome and simple, well-defined nervous system, the nematode C. elegans offers a unique opportunity to correlate gene expression with neuronal differentiation. The lineal origin, cellular morphology and synaptic connectivity of each of the 302 neurons are known. In many instances, specific behaviors can be attributed to particular neurons or circuits. Here we describe microarray-based methods that monitor gene expression in C. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL200

24 Samples

Download data: CEL, CHP, EXP

(Submitter supplied) Purpose: The goal of this study was to globally characterize the transcript levels of genes in Drosophila photoreceptor neurons. Using transcriptome profiling of isolated photoreceptor nuclei from day 10 flies, we identified genes with enriched or reduced transcript levels post nuclei isolation relative to the starting pre-isolation material (whole head RNA extracts).

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17275

6 Samples

Download data: CSV

(Submitter supplied) Purpose: The goal of this study was to globally characterize the transcript levels of genes in Drosophila photoreceptor neurons during aging. Using transcriptome profiling of isolated photoreceptor nuclei we analyzed the changes in gene expression that occur during aging over 5 time-points from 10 to 40 days post-eclosion and identified sets of genes with both up-regulated and down-regulated expression.

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17275

15 Samples

Download data: CSV

(Submitter supplied) http://0-www-nature-com.brum.beds.ac.uk/ng/journal/v33/n2/abs/ng1086.html. Little is known about broad patterns of variation and evolution of gene expression during any developmental process. Here we investigate variation in genome-wide gene expression among Drosophila simulans, Drosophila yakuba and four strains of Drosophila melanogaster during a major developmental transition?the start of metamorphosis. Differences in gene activity between these lineages follow a phylogenetic pattern, and 27% of all of the genes in these genomes differ in their developmental gene expression between at least two strains or species. more...

Organism:

Drosophila simulans; Drosophila yakuba; Drosophila melanogaster

Type:

Expression profiling by array

5 related Platforms

48 Samples

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(Submitter supplied) Little is known about broad patterns of variation and evolution of gene expression during any developmental process. Here we investigate variation in genome-wide gene expression among Drosophila simulans, Drosophila yakuba and four strains of Drosophila melanogaster during a major developmental transition-the start of metamorphosis. Differences in gene activity between these lineages follow a phylogenetic pattern, and 27% of all of the genes in these genomes differ in their developmental gene expression between at least two strains or species. more...

Organism:

Drosophila melanogaster; Drosophila simulans; Drosophila yakuba

Type:

Expression profiling by array

Platforms:

GPL227 GPL228

26 Samples

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(Submitter supplied) Drosophila simulans blue gut larvae vs. white prepupae Keywords: repeat sample

Organism:

Drosophila simulans; Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL228

4 Samples

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(Submitter supplied) Drosophila melanogaster Samarkand blue gut larvae vs. white prepupae Keywords: repeat sample

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL228

6 Samples

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(Submitter supplied) Drosophila melanogaster OregonR blue gut larvae vs. white prepupae Keywords: repeat sample

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL228

4 Samples

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(Submitter supplied) Drosophila melanogaster Netherlands2 blue gut larvae vs. white prepupae Keywords: repeat sample

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL228

4 Samples

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(Submitter supplied) Drosophila melanogaster CantonS blue gut larvae vs. white prepupae Keywords: repeat sample

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL228

4 Samples

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(Submitter supplied) Drosophila yakuba white prepupae vs. blue gut larvae Keywords: repeat sample

Organism:

Drosophila melanogaster; Drosophila yakuba

Type:

Expression profiling by array

Platform:

GPL227

4 Samples

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(Submitter supplied) Time course analysis series in Development of the transcriptome from Drosophila melanogaster using the Heidelberg FlyArray. All stages were hybridized against embryonic stage 0-4 h as reference control. Keywords: time-course

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL517

24 Samples

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(Submitter supplied) In Drosophila larvae, acquired synaptic thermotolerance following heat shock has previously been shown to correlate with the induction of heat shock proteins (Hsps) including HSP70. We tested the hypothesis that synaptic thermotolerance would be significantly diminished in a temperature-sensitive strain (hsf4) which has been reported not to be able to produce inducible Hsps in response to heat shock. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL311

6 Samples

Download data: TIFF

(Submitter supplied) whole larvae (all tissues) and tissue-specific transcriptome profiling by EC-tagging

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL7300

6 Samples

Download data: TXT

(Submitter supplied) Comparative analysis can provide important insights into complex biological systems. As demonstrated in the accompanying paper, Translating Ribosome Affinity Purification (TRAP), permits comprehensive studies of translated mRNAs in genetically defined cell populations following physiological perturbations. To establish the generality of this approach, we present translational profiles for twenty four CNS cell populations, and identify known cell-specific and enriched transcripts for each population. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

107 Samples

Download data: CEL

(Submitter supplied) We show that the combination of spatially restricted uracil phosphoribosyltransferase (UPRT) expression with 4-thiouracil (4TU) delivery can be used to label and purify cell type specific RNA from intact complex tissues. This method is useful for isolating RNA from cell types that are difficult to isolate by dissection or dissociation methods and should work in any organism where UPRT can be spatially expressed, including mammals and other vertebrates.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL8360

2 Samples

Download data: GPR