GEO DataSets

Analysis of 2 and 4 week old hearts ablated for menage-a-trois 1 (MAT1), a subunit of the cdk7/cyclin H/MAT1 heterotrimer. Results provide insight into the role of the cdk7/cyclin H/MAT1 complex in cardiac metabolism.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 age, 2 protocol sets

Platform:

GPL81

Series:

GSE6662

15 Samples

Download data: CEL

(Submitter supplied) The Cdk7/cyclin H/ménage-à-trois 1 (MAT1) heterotrimer has proposed functions in transcription as the kinase component of basal transcription factor TFIIH and is activated in adult hearts by hypertrophic pathways. Using cardiac-specific Cre, we ablated MAT1 in myocardium. Despite reduced Cdk7 activity, MAT1-deficient hearts grew normally. However, fatal heart failure ensued at 6-8 weeks. By microarray profiling, quantitative RT-PCR, and Western blotting at 4 weeks, genes for energy metabolism were found to be suppressed selectively, including targets of peroxisome proliferator-activated receptor-gamma coactivator-1 (PGC-1). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2561

Platform:

GPL81

15 Samples

Download data: CEL

(Submitter supplied) The following abstract from the submitted manuscript describes the major findings of this work. The metabolic development of high energy-utilizing organs such as the heart involves mitochondrial proliferation at birth followed by a maturation process during the postnatal period. Conditional gene targeting was used in mice to explore the role of the PPARgamma coactivator 1 (PGC-1) coactivators during postnatal development and in adult heart. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4776

Platform:

GPL6246

10 Samples

Download data: CEL

Analysis of heart from adults deficient for peroxisome proliferator-activated receptor γ coactivator-1 (PGC-1) α and PGC-1β. Adult PGC-1α/β deficient heart did not exhibit abnormal mitochondrial dynamics or heart failure. Results provide insight into role of PGC-1 coactivators in adult heart.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 genotype/variation sets

Platform:

GPL6246

Series:

GSE43798

10 Samples

Download data: CEL

(Submitter supplied) To identify mediators of obesity-linked reductions in PGC-1, we tested the effects of cellular nutrients in C2C12 myotubes. While overnight exposure to high insulin, glucose, glucosamine, or amino acids had no effect, saturated fatty acids (FA) potently reduced PGC-1a and b mRNA expression. Keywords: Nutrient Effect

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2648

Platform:

GPL8321

6 Samples

Download data: CEL

Analysis of myoblasts treated with the saturated fatty acid palmitate. Muscle expression of PPAR coactivator 1 (PGC-1) is reduced in models of obesity. Palmitate decreases the expression of PGC-1. Results provide insight into the molecular basis of the link between overnutrition, obesity, and PGC-1.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL8321

Series:

GSE6766

6 Samples

Download data: CEL

(Submitter supplied) Mitochondria play an essential role in the ability of brown fat to generate heat, and the PGC-1 coactivators control several aspects of mitochondrial biogenesis. To investigate their specific roles in brown fat cells, we generated immortal preadipocyte lines from the brown adipose tissue of mice lacking PGC-1α. We could then efficiently knockdown PGC-1β expression by shRNA expression. Loss of PGC-1α did not alter brown fat differentiation but severely reduced the induction of thermogenic genes. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2123

Platform:

GPL1261

6 Samples

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(Submitter supplied) To investigate the specific role of PGC-1 coactivators in brown fat cells, we generated immortal preadipocyte lines from the brown adipose tissue of mice lacking PGC-1alpha. We could then efficiently knockdown PGC-1beta expression by shRNA expression. Loss of PGC-1alpha did not alter brown fat differentiation but severly reduced the induction of thermogenic genes. In order to assess the specific requirement for PGC-1α in the global transcriptional response to cAMP, we used Affymetrix arrays to compare the sets of genes induced in response to a 4 hr dbcAMP treatment in differentiated wt and KO cells. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2149

Platform:

GPL1261

8 Samples

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Analysis of PGC-1alpha null brown adipocytes treated with cAMP for 4 hours. PGC-1alpha is required for the thermogenic function of brown fat cells, and cAMP plays a role in thermogenesis. Results provide insight into the role of PGC-1alpha in the global transcriptional response to cAMP.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE5041

8 Samples

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Analysis of brown fat cells lacking PGC-1alpha or both PGC-1alpha and PGC-1beta. PGC-1alpha is required for the thermogenic function of brown fat cells, and PGC-1beta is the closest homolog of PGC-1alpha. Results provide insight into the specific roles of PGC-1alpha and PGC-1beta in brown fat cells.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 agent, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE5042

6 Samples

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