GEO DataSets

Analysis of first instar larvae containing a chromosome 4 specific protein POF null mutation. POF is an autosome-specific protein that regulates gene expression in chromosome 4 in its entirety. Results provide insight into the molecular mechanisms that regulate chromosome 4 specific gene expression.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array, transformed count, 2 genotype/variation sets

Platform:

GPL72

Series:

GSE5984

7 Samples

Download data: CEL

(Submitter supplied) Analysis of gene expression in pof deletion mutants. Chromosome 4 genes are down-regulated in pof mutants compared to wildtype control. 200 Drosophila melanogaster first instar larvae were used for each of three biological replicates of y1 w67c23; PofD119/PofD119 and three biological replicates of y1 w67c23 as controls. Keywords: Mutant vs Wt expression

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Dataset:

GDS2619

Platform:

GPL1322

6 Samples

Download data: CEL

(Submitter supplied) Analysis of expression in pof mutant and wt 1st instar larvae Keywords: Mutant vs Wt expression

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Dataset:

GDS2627

Platform:

GPL72

7 Samples

Download data: CEL

Analysis of first instar larvae containing a chromosome 4 specific protein POF D119 mutation. POF is an autosome-specific protein that regulates gene expression in chromosome 4 in its entirety. Results provide insight into the molecular mechanisms that regulate chromosome 4 specific gene expression.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array, transformed count, 2 genotype/variation sets

Platform:

GPL1322

Series:

GSE6839

6 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Drosophila melanogaster

Type:

Expression profiling by genome tiling array

Platform:

GPL6629

23 Samples

Download data: CEL, CHP, TXT

(Submitter supplied) In Drosophila, two chromosome-wide compensatory systems have been characterized; the dosage compensation system acting on the male X-chromosome and the chromosome specific regulation of genes located on the heterochromatic 4th chromosome. Dosage compensation in Drosophila is accomplished by hypertranscription of the single male X-chromosome mediated by the MSL-complex. The mechanism for this compensation is suggested to be an MSL-complex mediated enhanced transcriptional elongation while the mechanism for the compensation mediated by Painting of fourth (POF) on the 4th chromosome has remained elusive. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by genome tiling array

Platform:

GPL6629

5 Samples

Download data: CEL, CHP

(Submitter supplied) In Drosophila, two chromosome-wide compensatory systems have been characterized; the dosage compensation system acting on the male X-chromosome and the chromosome specific regulation of genes located on the heterochromatic 4th chromosome. Dosage compensation in Drosophila is accomplished by hypertranscription of the single male X-chromosome mediated by the MSL-complex. The mechanism for this compensation is suggested to be an MSL-complex mediated enhanced transcriptional elongation while the mechanism for the compensation mediated by Painting of fourth (POF) on the 4th chromosome has remained elusive. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by genome tiling array

Platform:

GPL6629

6 Samples

Download data: CEL, CHP

(Submitter supplied) In Drosophila, the global increase in transcription from the X chromosome in males to compensate for its monosomy is mediated by the male-specific-lethal complex (MSL-C) consisting of five proteins and two non-coding RNAs, roX1 and roX2. After an initial sequence dependent recognition by the MSL-C of 150-300 high affinity sites, the spreading to the majority of the X-linked genes depends on local MSL-C concentration and active transcription. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by genome tiling array

Platform:

GPL6629

12 Samples

Download data: CEL, CHP, TXT

(Submitter supplied) Two chromosome-targeting and gene regulatory systems are present in Drosophila melanogaster. The male X-chromosome is targeted by the male-specific lethal complex believed to mediate the two-fold up-regulation of the X-linked genes. The highly heterochromatic 4th chromosome is specifically targeted by the Painting of Fourth (POF) protein which together with heterochromatin protein 1 (HP1) modulate the expression level of genes on the 4th chromosome. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by genome tiling array; Genome binding/occupancy profiling by genome tiling array

Platform:

GPL5404

19 Samples

Download data

(Submitter supplied) Two chromosome-targeting and gene regulatory systems are present in Drosophila melanogaster. The male X-chromosome is targeted by the male-specific lethal complex believed to mediate the two-fold up-regulation of the X-linked genes. The highly heterochromatic 4th chromosome is specifically targeted by the Painting of Fourth (POF) protein which together with heterochromatin protein 1 (HP1) modulate the expression level of genes on the 4th chromosome. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by genome tiling array

Platform:

GPL5404

4 Samples

Download data

(Submitter supplied) Two chromosome-targeting and gene regulatory systems are present in Drosophila melanogaster. The male X-chromosome is targeted by the male-specific lethal complex believed to mediate the two-fold up-regulation of the X-linked genes. The highly heterochromatic 4th chromosome is specifically targeted by the Painting of Fourth (POF) protein which together with heterochromatin protein 1 (HP1) modulate the expression level of genes on the 4th chromosome. more...

Organism:

Drosophila melanogaster

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL5404

15 Samples

Download data: TXT

(Submitter supplied) In Drosophila melanogaster, two chromosome-specific targeting and regulatory systems have been described. The male-specific lethal (MSL) complex supports dosage compensation by stimulating gene expression from the male X-chromosome and the protein Painting of fourth (POF) specifically targets and stimulates expression from the heterochromatic 4th chromosome. The targeting sites of both systems are well characterized, but the principles underlying the targeting mechanisms have remained elusive. more...

Organism:

Drosophila melanogaster

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL6629

1 Sample

Download data: CEL, CHP

(Submitter supplied) The prominent role of pericentromeric Mbp block of 1.688 satellites in hybrid incompatibility, facilitating role of distributed X-linked 1.688 satellites in dosage compensation in males and being as a necessary element for POF recruitment to chromosome X in females led us to further investigate the role of 1.688 satellites and POF in chromosome-wide gene regulation by comparing RNA-seq data from the relevant mutants in comparison to wildtype.

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17275

32 Samples

Download data: TXT

(Submitter supplied) Chromosomal instability which involves deletion and duplication of chromosomes or chromosome parts is a common feature of cancers, and deficiency screens are commonly used as a method to find genes involved in different biological pathways. Still, how gene expression from whole chromosomes or large chromosomal domains is affected by deficiencies, duplications or chromosome loss is largely unknown. Using expression microarrays of deficiency hemizygotes and a duplication hemizygote we show that expressed genes are significantly buffered when present in a deficiency hemizygote and that the buffering effect is general and not mainly caused by feedback regulation of individual genes. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL1322

27 Samples

Download data: CEL

(Submitter supplied) To study the influence of POF on transcription in testis we made expression arrays from dissected testes (Pof mutants and wild type control). It is clear that POF mainly alters the expression of genes on the fourth chromosome Keywords: mutant analysis

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL1322

6 Samples

Download data: CEL

(Submitter supplied) mRNA expression levels were determined by NGS for wildtype larvae as well as for larvae lacking HP1a [Su(var)205^04/Su(var)205^05 transheterozygotes].

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13304

4 Samples

Download data: TXT

(Submitter supplied) Heterochromatin protein 1a (HP1a) is a chromatin associated protein that has been well studied in many model organisms, such as Drosophila, where it is a determining factor for classical heterochromatin. HP1a is associated with the two histone methyltransferases SETDB1 and Su(var)3-9, which mediate H3K9 methylation marks and participate in the establishment and spreading of HP1a enriched chromatin. While HP1a is generally regarded as a factor that represses gene transcription, several reports have linked HP1a binding to active genes, and in some cases, it has been shown to stimulate transcriptional activity. To clarify the function of HP1a in transcription regulation and its association with Su(var)3-9, SETDB1 and the chromosome 4 specific protein POF, we conducted genome-wide expression studies and combined the results with available binding data in Drosophila melanogaster. The results suggested that HP1a has a repressing function on chromosome 4, where it preferentially targets non-ubiquitously expressed genes (NUEGs), and a stimulating function in pericentromeric regions. Further, we showed that the effects of SETDB1 and Su(var)3-9 are similar to HP1a, and on chromosome 4, Su(var)3-9, SETDB1 and HP1a target the same genes. In contrast, transposons are repressed by HP1a and Su(var)3-9 but are un-affected by SETDB1 and POF. In addition, we found that the binding level and expression effects of HP1a are affected by gene length. Our results indicate that genes have adapted to be properly expressed in their local chromatin environment.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL1322

18 Samples

Download data: CEL

(Submitter supplied) Drosophila heterochromatin protein 1- HP11 is believed to be involved in active transcription, transcriptional gene silencing, and the formation of heterochromatin2-7. However, little is known about the function of HP1 during development. Using a Gal4-induced RNA interference system, we show that conditional depletion of HP1 in transgenic flies results in preferential lethality in male flies. Cytological analysis of mitotic chromosomes reveals that HP1 depletion causes sex-biased chromosomal defects, including telomere fusions. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Dataset:

GDS1526

Platform:

GPL1322

11 Samples

Download data

Analysis of male and female 3rd instar larvae following depletion of heterochromatin protein 1 (HP1) using a Gal4-induced RNA interference system. Results provide insight into the function of HP1 during development and a sex-specific role.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array, transformed count, 2 gender, 3 genotype/variation, 2 protocol sets

Platform:

GPL1322

Series:

GSE3055

11 Samples

Download data

(Submitter supplied) roX RNAs are involved in the chromosome-wide gene regulation that occurs during dosage compensation in Drosophila. Dosage compensation equalizes expression of X-linked and autosomal genes. Drosophila males increase transcription two-fold from their single X chromosome. This is mediated by the MSL complex, which is composed of the male-specific lethal (MSL) proteins and two noncoding roX RNAs, roX1 and roX2. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL1322

6 Samples

Download data: CEL