GEO DataSets

Temporal analysis of two nonmalignant mammary epithelial cells (HMEC) grown in a laminin-rich extracellular matrix. Both HMECs transit from a disorganized to an organized state to form polarized acini. Results identify gene signatures with potential value for breast cancer prognosis prediction.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 cell line, 2 other, 3 time sets

Platform:

GPL96

Series:

GSE8096

12 Samples

Download data: CEL

(Submitter supplied) Nonmalignant human mammary epithelial cells (HMEC) seeded in laminin-rich extracellular matrix (lrECM) form polarized acini and, in doing so, transit from a disorganized proliferating state to an organized growth-arrested state. We hypothesized that the gene expression pattern of organized and growth-arrested HMECs would share similarities with breast tumors with good prognoses. Using Affymetrix HG-U133A microarrays, we analyzed the expression of 22,283 gene transcripts in 184 (finite life span) and HMT3522 S1 (immortal nonmalignant) HMECs on successive days after seeding in a lrECM assay. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2810

Platform:

GPL96

12 Samples

Download data: CEL

(Submitter supplied) MicroRNAs are likely to play pivotal roles in both stem cells and cancer, and are further promising candidates for future therapeutic purposes. To understand the involvement of microRNAs throughout mammary cell differentiation, and the association between microRNAs, stem cells and cancer we profiled the expression of all known microRNA in different stages of mammary cell differentiation.

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array

Platform:

GPL15409

4 Samples

Download data: TXT

(Submitter supplied) This study was performed to check that ESR1 and BMI1 are biologically active after lentiviral transduction of primary human mammary epithelial cells (HMECs) with lentiviral vectors expressing ESR1 and BMI1 from the human PGK promoter. ESR1 targets like PGR, PRLR and GREB1, but not TFF1 and XBP1, were induced by estradiol in the ESR1-expressing cells. BMI1 targets like BMI1, NEFL and CCND2 were repressed in the BMI1-expressing cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

8 Samples

Download data: CEL

(Submitter supplied) The Epidermal Growth Factor Receptor 2 (ERBB2 or HER2) is amplified and overexpressed in approximately 20% of invasive breast cancers and is associated with metastasis and poor prognosis. Here we describe the role of a constitutively active splice variant of HER2 (Delta-HER2) in human mammary epithelial cells. Overexpression of Delta-HER2 in human mammary cells decreased apoptosis and increased proliferation and expression of epithelial-to-mesenchymal markers. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

9 Samples

Download data: CEL

(Submitter supplied) In this study, we profiled gene expressions on 20 biopsy tissues of early stage breast carcinoma using Applied Biosystem’s Human Genome Survey Microarrays. Two main previously defined clinically relevant subtypes of breast tumors, Luminal A (longest survival time) and Basal (shortest survival time) were identified. Statistical analysis identified 1210 genes as signature genes characterizing the two subtypes of breast cancer. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL1843 GPL1708 GPL1426

80 Samples

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