GEO DataSets

Analysis of intestinal crypts following inactivation of beta catenin. The mucosa of the intestine is composed of submucosal invaginations called intestinal crypts. Results provide insight into the molecular mechanisms underlying crypt loss as a result of beta-catenin inactivation.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 protocol sets

Platform:

GPL1261

Series:

GSE8818

4 Samples

Download data: CEL, CHP

(Submitter supplied) The Wnt signaling pathway is deregulated in over 90% of human colorectal cancers. β Catenin, the central signal transducer of the Wnt pathway, can directly modulate gene expression by interacting with transcription factors of the TCF/LEF-family. In the present study we investigate the role of Wnt signaling in the homeostasis of intestinal epithelium using tissue-specific, inducible beta-catenin gene ablation in adult mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2984

Platform:

GPL1261

4 Samples

Download data: CEL, CHP

(Submitter supplied) Gene expression profiles in hepatoma cells Keywords: Cell type comparison

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL2913

36 Samples

Download data

(Submitter supplied) Gene expression profiles in hepatoma cells Keywords: Cell type comparison

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL2912

18 Samples

Download data

(Submitter supplied) Gene expression profiles in hepatoma cells Keywords: Cell type comparison

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL2911

18 Samples

Download data

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

16 Samples

Download data: CEL

(Submitter supplied) To investigate the impact of ablating Bcl9/Bcl9l on tumorigenesis, 6-8- week-old mice were exposed first to a single dose dimethylhydrazine (DMH, 44 mg/kg body weight), which is metabolized in the liver to carcinogenic azoxymethane (AOM), followed by 7 days oral administration of 2 % dextrane sulfate sodium (DSS) in the drinking water. This regimen results in the emergence of dysplastic adenomas, which progress to differentiated adenocarcinomas that are morphologically similar to human colorectal adenocarcinomas and typically harbor β-catenin stabilizing mutations of GSK3ß phosphorylation sites. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

10 Samples

Download data: CEL

(Submitter supplied) To assess potential changes in Wnt signaling more comprehensively, EDTA-dissociated colon epithelial cells from three pools of wild-type and Bcl9/Bcl9l-mutant mice were subjected to an exploratory comparative gene expression profiling.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL

(Submitter supplied) Embryonic stem (ES) cells have the potential to generate a variety of cell lineages including endothelial cells, blood cells and smooth muscle cells. flk1-expressing cells derived from ES cells serve as vascular progenitors. We have used global gene expression analysis in order to establish a comprehensive list of candidate genes in the developing vasculature during ES cell differentiation in vitro. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2322

Platform:

GPL922

24 Samples

Download data

Analysis of VEGF receptor Flk1-expressing cells isolated from embryoid bodies up to 8 days post-differentiation. Flk1-expressing cells have developmental potential uniquely restricted to hematopoietic and endothelial lineages. Results provide insight into early vascular development.

Organism:

Mus musculus

Type:

Expression profiling by array, log2 ratio, 2 genotype/variation, 3 time sets

Platform:

GPL922

Series:

GSE3757

24 Samples

Download data

(Submitter supplied) During kidney development segmented epithelia of the nephron derive from progenitor cells in the metanephric mesenchyme after induction by secreted molecules from the ureteric bud. We have identified three distinct inductive activities from a ureteric bud cell line. These include leukemia inhibitory factor (LIF), neutrophil gelatinase-associated lipocalin (NGAL) and an active fraction currently referred to as ANX. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

42 Samples

Download data

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL24247 GPL17021 GPL19057

25 Samples

Download data: NARROWPEAK, TXT

(Submitter supplied) The homeostasis of the gut epithelium relies upon continuous renewal and proliferation of crypt-resident intestinal epithelial stem cells (IESCs). Wnt/β-catenin signaling is required for IESC maintenance, however, it remains unclear how this pathway selectively governs the identity and proliferative decisions of IESCs. Here, we demonstrate that C-terminally-recruited transcriptional co-factors of β-catenin act as all-or-nothing regulators of Wnt-target gene expression. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

6 Samples

Download data: TXT

(Submitter supplied) The homeostasis of the gut epithelium relies upon continuous renewal and proliferation of crypt-resident intestinal epithelial stem cells (IESCs). Wnt/β-catenin signaling is required for IESC maintenance, however, it remains unclear how this pathway selectively governs the identity and proliferative decisions of IESCs. Here, we demonstrate that C-terminally-recruited transcriptional co-factors of β-catenin act as all-or-nothing regulators of Wnt-target gene expression. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

13 Samples

Download data: CSV

(Submitter supplied) The homeostasis of the gut epithelium relies upon continuous renewal and proliferation of crypt-resident intestinal epithelial stem cells (IESCs). Wnt/β-catenin signaling is required for IESC maintenance, however, it remains unclear how this pathway selectively governs the identity and proliferative decisions of IESCs. Here, we demonstrate that C-terminally-recruited transcriptional co-factors of β-catenin act as all-or-nothing regulators of Wnt-target gene expression. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL17021

6 Samples

Download data: NARROWPEAK

(Submitter supplied) We report the transcriptomic alterations of Lgr5-eGFP+ intestinal stem cells upon Wnt and R-spondin gain-of-function and loss-of-function in vivo

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

14 Samples

Download data: MTX, TSV

(Submitter supplied) sorted Lgr5-eGFP+ cells under conditions of in vivo Rspo manipulation in reporter mice,

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

10 Samples

Download data: XLSX

(Submitter supplied) Background: Wnt signaling maintains the undifferentiated state of intestinal crypt progenitor cells by inducing the formation of nuclear TCF4/beta-catenin complexes. In colorectal cancer, activating mutations in Wnt pathway components cause inappropriate activation of TCF4/beta-catenin-driven transcription. Despite the passage of a decade after the discovery of TCF4 and beta-catenin as the molecular effectors of the Wnt signal, few transcriptional activators essential and unique to the regulation of this transcription program have been found. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4133

12 Samples

Download data: TXT

(Submitter supplied) Wnt/b-catenin signaling supports intestinal homeostasis by regulating proliferation in the crypt. Multiple Wnts are expressed in Paneth as well as other intestinal epithelial and stromal cells. Ex vivo, Wnts secreted by Paneth cells can support intestinal stem cells when Wnt signaling is enhanced with supplemental R-Spondin 1 (RSPO1). However, in vivo, the source of Wnts in the stem cell niche is less clear. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11017

18 Samples

Download data: TXT

(Submitter supplied) Differential GRO-Seq and ChIP-Seq (H3K27me3) was performed on mouse mini-gut model cells.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13112

10 Samples

Download data: TXT