GEO DataSets

Analysis of maturing testes from animals suppressed for follicle stimulating hormone (FSH) activity from age 14 to 18 days. FSH activity suppressed by treatment with an antibody to FSH (FSHAb). Results provide insight into the role of FSH in Sertoli and male germ cell development.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL85

Series:

GSE10293

4 Samples

Download data: CEL

(Submitter supplied) To unravel the genes that underpin the effects of FSH on spermatogenesis, testicular gene expression was compared in juvenile rats after FSH suppression to littermate controls. Based on existing data, it is apparent that the dominant function of FSH shifts between 9 and 18 day postpartum (dpp) during the first wave of spermatogenesis from driving Sertoli cell proliferation to support of germ cells. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS3189

Platform:

GPL85

4 Samples

Download data: CEL

(Submitter supplied) Spermatogonial stem cells are quiescent, undergo self-renewal or differentiating divisions, thereby forming the cellular basis of spermatogenesis. This cellular development is orchestrated by follicle-stimulating hormone (FSH), through the production of Sertoli cell-derived factors, and by Leydig cell-released androgens. Here, we investigate the transcriptional events induced by Fsh in a steroid-independent manner on the restart of zebrafish (Danio rerio) spermatogenesis ex vivo, using testis from adult males where type A spermatogonia were enriched by estrogen treatment in vivo. more...

Organism:

Danio rerio

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18413

12 Samples

Download data: TXT

(Submitter supplied) Spermatogenesis requires the presence of functional somatic Sertoli cells in the seminiferous tubules of the testis. Sertoli cells provide support and factors necessary for the successful progression of germ cells into spermatozoa. Sertoli cells are regulated to a large degree by the glycoprotein hormone FSH, which is required for the testis to acquire full size and spermatogenic capacity. Signaling events initiated by the binding of FSH to its receptor lead to an alteration of Sertoli cell gene expression. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS174

Platform:

GPL85

10 Samples

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Analysis of gene expression in follicle-stimulating hormone (FSH) treated Sertoli cells. Sertoli cells from 20 day rats were cultured in the presence of 25 ng/ml ovine FSH and gene expression monitored 0, 2, 4, 8 and 24 hours after addition of FSH.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 5 time sets

Platform:

GPL85

Series:

GSE509

10 Samples

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(Submitter supplied) Spermatogenesis is a complex process involving multiple cells and cellular signals. Among all other cells, Sertoli cells (Sc) are absolutely indispensable for sperm production because they provide biochemical signal and physical support to developing germ cells in the testis. Sc drastically transform during the process of attainment of reproductive maturity. Stage specific gene expression by Sc, supplement for the changing need of developing germ cells. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL7294

9 Samples

Download data: TXT

(Submitter supplied) In all vertebrates, the dual function of testis (production of sexual steroids and production of gametes) is mainly regulated by two gonadotropic pituitary hormones, FSH and LH. However, in fish the biological activities of the two hormones are not still clearly delineated and moreover, their molecular mechanisms are yet poorly understood. In this study we investigated the effects of FSH and LH on testicular gene expression, in the rainbow trout, at two developmental stages I-II and III (I: spermatogonia only, II: active spermatogonia proliferation, III: meiosis onset with the appearance of spermatocytes and round spermatids).

Organism:

Oncorhynchus mykiss

Type:

Expression profiling by array

Platform:

GPL3650

42 Samples

Download data: TXT

(Submitter supplied) Gene expression profiles of somatic cells of fetal and adult testis. To identify genes developmentally regulated in the somatic cells of the testis, serial analysis of gene expression (SAGE) has been used to generate gene expression profiles from these cells in the fetal and adult mouse. To avoid germ cell transcripts, a fetal SAGE library was generated from germ cell-free fetal Wv/Wv mice, and an adult SAGE library was generated from adult testes depleted of germ cells with busulfan. more...

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platform:

GPL11

2 Samples

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(Submitter supplied) In the normal mouse the pituitary gonadotrophins determine development, maturation and physiological regulation of the testis with follicle-stimulating hormone (FSH) activating the Sertoli cell and luteinising hormone (LH) the Leydig cell. To look at the potential interaction of cell types within the testis following hormone stimulation we have investigated the effect of rFSH on testicular gene expression in the hypogonadal (hpg) male mouse. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL339

18 Samples

Download data: CEL

(Submitter supplied) The ability of male reproduction is seriously dependent on Sertoli cells. However, the mechanisms governing the functional integrity of Sertoli cells remained largely unexplored. Tyrosine phosphatase protein Shp2 is expressed in germ, Leydig and Sertoli cells of mice testes. But the physiological role of Shp2 in the spermatogenesis was not fully understood. Thus, we conditionally deleted Shp2 gene in Sertoli cells using two transgenic models, and demonstrated that Shp2 deficiency caused infertility, excessive differentiation of SSCs and abnormal BTB in mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

8 Samples

Download data: CEL

(Submitter supplied) Early reduction of testicular estrogen synthesis induces increased Sertoli cell proliferation. This experiment was an initial analysis of potential changes in gene expression and included four litters with six boars in each litter.Boars were derived from PIC genetic lines. Half of the boars in each litter were orally treated with the aromatase inhibitor letrozole (0.1 mg/kg) once per week, a teatment that inhibits aromatase by greateer than 90% at this age. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19176

24 Samples

Download data: XLSX

(Submitter supplied) Analysis of the transcriptome of testes of hypogonadal mice that had been pretreated with testosterone proprionate for 2 weeks then submitted to testosterone withdrawal. Keywords = testis, hypogonadal mice, testosterone Keywords: other

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL81

4 Samples

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(Submitter supplied) To investigate whether early postnatal stress has a long-lasting impact on Sertoli cells up to adulthood, we compared the gene expression of adult Sertoli cells obtained from stressed versus control mice.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

13 Samples

Download data: CSV