GEO DataSets

Analysis of growth plate chondrocytes, representing reserve and hypertrophic zones (RZ and HZ, respectively), isolated from metacarpals of prepubertal males. RZ cells terminally differentiate into non-dividing HZ cells. Results provide insight into the molecular basis of chondrocyte development.

Organism:

Bos taurus

Type:

Expression profiling by array, transformed count, 2 cell type sets

Platform:

GPL2112

Series:

GSE18738

12 Samples

Download data: CEL

(Submitter supplied) Growth plate chondrocytes were isolated from the distal metacarpus of young dairy cattle (all under 10 mo of age), the chondrocytes were released from the extracellular matrix by digestion with Collagenase P for 4 hours, and the various zones of the growth plate were separated by density centrifugation. The least-dense Hypertrophic Zone (HZ) cells were compared to the most-dense Reserve Zone (RZ) cells. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Dataset:

GDS3733

Platform:

GPL2112

12 Samples

Download data: CEL

(Submitter supplied) Human growth plate chondrocytes were isolated from the distal femoral growth plate from 3 different adolescents undergoing epiphysiodesis. Cell were isolated and grown in monolayers, long term, in 20% serum. Cells were replated and cultured 48 h in 0.5% serum. Keywords: repeat sample

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL8300

3 Samples

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(Submitter supplied) Human growth plate chondrocytes were isolated from the distal femoral growth plate from a single adolescent undergoing epiphysiodesis. Cell were isolated and grown in monolayers, long term. Cells were replated and cultured in 0.5% or 20% serum. Keywords: parallel sample

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL8300

2 Samples

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(Submitter supplied) Longitudinal bone growth depends upon the execution of an intricate series of cellular activities by epiphyseal growth plate chondrocytes. In order to better understand these coordinated events, microarray analysis was used to compare gene expression in chondrocytes isolated from the proliferative and hypertrophic zones of the avian growth plate. In this experiment we compared pooled samples of proliferative and hypertrophic chondrocytes isolated from the chick growth plate. more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3213

8 Samples

Download data: CEL

(Submitter supplied) Objectives: To identify similarities and differences in gene expression data in the MEK/ERK and PI3K pathways and to determine how histone modification affects these same pathways. Goal: To identify and functionally characterize novel targets of these signaling pathways in the context of chondrocyte differentiation. Keywords: Treatment, signaling, one-colour array, signaling pathway comparison

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

15 Samples

Download data: CEL, CHP

(Submitter supplied) Objectives: To identify similarities and differences in gene expression data in the MEK/ERK and PI3K pathways and to determine how histone modification affects these same pathways. Goal: To identify and functionally characterize novel targets of these signaling pathways in the context of chondrocyte differentiation. Keywords: Teatment, signaling, one-colour array, signaling pathway comparison

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

15 Samples

Download data: CEL

(Submitter supplied) In the growth plate, the reserve and perichondral zones have been hypothesized to have similar functions, but their exact functions are poorly understood. Our hypothesis was that significant differential gene expression exists between perichondral and reserve chondrocytes that may differentiate the respective functions of these two zones. Normal Sprague-Dawley rat growth plate chondrocytes from the perichondral zone (PC), reserve zone (RZ), proliferative zone (PZ), and hypertrophic zone (HZ) were isolated by laser microdissection and then subjected to microarray analysis. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

8 Samples

Download data: CEL, CHP

(Submitter supplied) Human adult height reflects the outcome of childhood skeletal growth. Growth plate (epiphyseal) chondrocytes are key determinants of height. As epiphyseal chondrocytes mature and proliferate, they pass through three developmental stages, which are organized into three distinct layers in the growth plate: (i) resting (round), (ii) proliferative (flat), and (iii) hypertrophic. Recent genomewide association studies (GWASs) of human height identified numerous associated loci, which are enriched for genes expressed in growth plate chondrocytes. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16417

4 Samples

Download data: XLSX