GEO DataSets

Temporal analysis of macrophage-like THP-1 cell line infected by Mycobacterium tuberculosis (Mtb) W-Beijing strains and H37Rv. Mtb W-Beijing sublineages are highly virulent, prevalent and genetically diverse. Results provide insight into host macrophage immune response to Mtb W-Beijing strains.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 3 infection, 13 strain, 4 time sets

Platform:

GPL570

Series:

GSE29628

40 Samples

Download data: CEL

(Submitter supplied) The W-Beijing family of Mycobacterium tuberculosis (Mtb) strains is known for its high-prevalence and -virulence, as well as for its genetic diversity, as recently reported by our laboratories and others. However, little is known about how the immune system responds to these strains. To explore this issue, here we used reverse engineering and genome-wide expression profiling of human macrophage-like THP-1 cells infected by different Mtb strains of the W-Beijing family, as well as by the reference laboratory strain H37Rv. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4258

Platform:

GPL570

40 Samples

Download data: CEL

(Submitter supplied) THP-1 cells were differentiated into macrophages with 0,1 µM PMA overnight then infected for 2 days with either H37Rv, M. marinum or heat killed H37Ra. Then RNA was collected in order to quantify the inflammatory response transcription as compared with the relative uninfected controls.

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL29639

22 Samples

Download data: TXT

(Submitter supplied) We examined the microRNA profiles of THP-1 macrophages upon the MTB infection of (1) Beijing/W and non-Beijing/W clinical strains, and (2) susceptible and multidrug-resistant (MDR-) MTB strains.

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL19763

14 Samples

Download data: TXT

(Submitter supplied) the microRNA profiles of the host macrophages were studied by microarray in a small cohort with active MTB disease, latent infection (LTBI), and from healthy controls.

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL19763

10 Samples

Download data: TXT

(Submitter supplied) The innate immune system provides the first response to pathogen infection and orchestrates the activation of the adaptive immune system. Though a large component of the innate immune response is common to all infections, pathogen-specific innate immune responses have been documented as well. The innate immune response is thought to be especially critical for fighting infection with Mycobacterium tuberculosis (MTB), the causative agent of tuberculosis (TB). more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

156 Samples

Download data: TXT

(Submitter supplied) We infect bone marrow derived macrophages (BMDMs) with either a Hyper or Hypovirulent Mycobacterium tuberculolsis strain and assess the host response to these bacteria through RNAseq

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15907

9 Samples

Download data: TXT

(Submitter supplied) Host macrophage transcriptional responses to intracellular pathogens remain poorly characterized. We screened transcriptional enhancers engaged in response to M. tuberculosis (Mtb) infection by ChIPseq analysis of histone H3 lysine 4 monomethylation (H3K4me1). De novo monomethylation during infection was associated with genes implicated in host defense and apoptosis. These regions were enriched for binding sites for ETS transcription factor family members and response elements for nuclear receptors, including liver X receptors (LXRs) and peroxisomal proliferator activated receptors (PPARs), many of which were encompassed by transposable elements. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16686

6 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL13712

27 Samples

Download data: CEL

(Submitter supplied) How the complex interaction between Mycobacterium tuberculosis (Mtb) and the host is regulated during infection is still not well understood. Using a systems biology approach, we demonstrate here that miR-155 is one of several microRNAs that regulate host gene expression over the first 48 hours of Mtb infection in macrophages. miR-155 regulates the cell survival of Mtb-infected macrophages through SHIP1/AKT signaling. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL13712

12 Samples

Download data: CEL

(Submitter supplied) How the complex interaction between Mycobacterium tuberculosis (Mtb) and the host is regulated during infection is still not well understood. Using a systems biology approach, we demonstrate here that miR-155 is one of several microRNAs that regulate host gene expression over the first 48 hours of Mtb infection in macrophages. miR-155 regulates the cell survival of Mtb-infected macrophages through SHIP1/AKT signaling. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL13712

15 Samples

Download data: CEL

(Submitter supplied) Macrophages play an essential role in the early immune response to Mycobacterium tuberculosis and are the cell type preferentially infected in vivo. Primary macrophages and macrophage-like cell lines are commonly used as infection models, although the physiological relevance of cell lines, particularly for host-pathogen interaction studies, is debatable. Here we use high-throughput RNA-sequencing to analyse transcriptome dynamics of two macrophage models in response to M. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

36 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mycobacterium tuberculosis

Type:

Expression profiling by array

Platform:

GPL4057

33 Samples

Download data: GPR

(Submitter supplied) During lung infection Mycobacterium tuberculosis (Mtb) resides in macrophages and subverts the bactericidal mechanisms of these professional phagocytes. In this work we have analyzed by DNA microarray technique the global transcription profile of Mtb infecting primary human macrophages in order to identify putative bacterial pathogenic factors that can be relevant for the intracellular survival of Mtb. more...

Organism:

Mycobacterium tuberculosis

Type:

Expression profiling by array

Platform:

GPL4057

11 Samples

Download data: GPR

(Submitter supplied) This experiment is to compare the transcription patterns of mouse macrophages (J774A.1) infected with BCG, H37Ra and M. smegmatis under high multiplicity of infection (MOI). Through the global transcriptome profiling study, we define a pathogen specific host gene expression pattern and indicate that SRC likely plays a central role in regulating multiple unique signaling pathways activated by MTB infection. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5634

Platform:

GPL1261

4 Samples

Download data: CEL, CHP

Analysis of macrophage cell line J774A.1 infected with pathogenic mycobacteria BCG and H37Ra and non-pathogenic M. smegmatis under high multiplicity of infection (MOI = 50). Results provide insight into the molecular response of macrophage host cells to pathogenic and non-pathogenic mycobacteria.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 infection, 4 species sets

Platform:

GPL1261

Series:

GSE45675

4 Samples

Download data: CEL, CHP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus; Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL6480 GPL10787

118 Samples

Download data: TXT

(Submitter supplied) We have conducted experiments to compare transcriptional response to tuberculosis (TB) in THP1 cells in time points 1hr,6hr and 24hr after infection.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

66 Samples

Download data: TXT

(Submitter supplied) We have conducted experiments to compare transcriptional response to tuberculosis (TB) in whole blood in two different TB murine models (susceptible 129S2 strain and resistant C57BL/6 strain) in time points day 1, day 7, day 14 and day 21 after infection.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10787

52 Samples

Download data: TXT

(Submitter supplied) Background: Tuberculosis (TB) remains a major public health problem, especially in developing countries, with 1.5 million deaths annually worldwide. Antibiotics are commonly used in the treatment of bacterial infections. As with most drugs, antibiotic treatment can also alter host metabolism, leading to adverse side-effects. Antibiotics can also interfere with the immune system, indirectly through the disturbance of the body’s microbiota or directly by modulating the functions of immune cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

56 Samples

Download data: TXT