GEO DataSets

Analysis of livers from promyelocytic leukemia (Pml)-knockouts fed a high fat diet (HFD) for 20 wks. The increased predisposition of Pml-KOs to HFD-induced obesity is associated with aspects of metabolic syndrome. Results provide insight into molecular basis of a metabolic prosurvival role for PML.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 genotype/variation, 2 protocol sets

Platform:

GPL11180

Series:

GSE39220

12 Samples

Download data: CEL, CHP

(Submitter supplied) Cancer cells exhibit an aberrant metabolism which facilitates more efficient production of biomass, and hence tumor growth and progression. The genetic cues modulating this metabolic switch remain largely undetermined, however. Here we identify a metabolic function for PML which reveals an unexpected role for this bona-fide tumor suppressor in pro-survival activity in breast cancer. We find that PML acts as both a negative regulator of PGC1A acetylation and as a potent activator of peroxisome proliferator-activated receptor (PPAR) signaling and fatty acid oxidation (FAO). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4771

Platform:

GPL11180

12 Samples

Download data: CEL, CHP

(Submitter supplied) To study the potential target genes regulated by PML in endothelial cells, we carried out siRNA-mediated knockdown of PML in HUVEC cells. To eliminate the off-target effects of siRNAs, we utilized two different siRNAs. Only the genes changed in the same pattern following both siRNAs transfection are considered as potential PML-knockdown responsive genes.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6104

6 Samples

Download data: TXT

(Submitter supplied) To study the interaction effects between promyelocytic leukemia (PML) gene knockdown and tumor necrosis factor-alpha (TNFalpha) signaling in human umbilical vein endothelial cells (HUVECs), we transfected control or two independent PML siRNAs into HUVEC cells without or with 20 ng/mL TNFalpha treatment for 20 h. The total RNA was extracted for gene expression microarray analyses.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6104

12 Samples

Download data

(Submitter supplied) The multitasking ProMyelocytic Leukemia (PML) protein, which was originally recognized as tumor suppressive factor, is commonly lost in primary breast cancer tissue samples. However, PML’s role in breast cancer is still debated, as high PML levels reportedly correlate with cancer cell pro-survival activity and poor patient prognosis in a subset of breast cancer types. To study the molecular mechanisms underlying the involvement of PML in cell proliferation and self-renewal pathways in breast cancer, a transgene expressing PMLIV upon doxycycline treatment was stably integrated in MDA-MB-231 cells (tetPMLIV MDA-MB-231 cells), a claudin-low, triple negative, aggressive breast cancer cell line. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

8 Samples

Download data: CEL, CHP

(Submitter supplied) MMTV-PyVT Mammary tumors were harvested from PyVT-wild-type (WT), PyVT-Nr4a1 knockout (KO) littermates on a congenic FVB/NJ background,minced and incubated with collagenase type I digestion solution .The digested Mammary tumor cells were collected and maintained in DMEM medium containing 10% FBS for 24 hour.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

6 Samples

Download data: TXT

(Submitter supplied) Mutations in the gene encoding lipin 1 cause hepatic steatosis in fld mice, a genetic model of lipodystrophy. Lipin 1 appears to be highly involved in the control of fatty acid metabolism. Lipin 1 is most often located in the nucleus, but other studies suggest that lipin also has effects in the cytoplasm. However, the molecular function of lipin 1 is unclear. To evaluate the effects of activation of the lipin 1 system in liver, lipin 1beta was overexpressed in mouse liver using an adenoviral vector. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2291

Platform:

GPL339

4 Samples

Download data

Analysis of livers of transgenics overexpressing lipin 1-beta, an alternative form of lipin 1 containing a 33 amino acid insertion. Mutations in the gene encoding lipin 1 cause hepatic steatosis in fld animals, a genetic model of lipodystrophy. Results provide insight into the function of lipin 1.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation sets

Platform:

GPL339

Series:

GSE5538

4 Samples

Download data

(Submitter supplied) Here, we show that short term HFD feeding did not change the cardiac function in normal (Con) and increased FAO mice (ACC2 iKO). RNA sequencing analysis show that ACC2 can have small but significant genetic perturbations impact on the global transcriptome under chow and HFD condition. Most of fatty acid degradation and PPAR signaling pathway related gene transcription levels were decreased by knocking out ACC2. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

20 Samples

Download data: CSV