GEO DataSets

Analysis of Huh7, HepG2, and Hep3B hepatocellular carcinoma (HCC) cells depleted for the ubiquitin modulator COP1. COP1 regulates p53 activity by ubiquitination. p53 is wild type in HepG2, mutated in Huh7, and lacking in Hep3B. Results provide insight into the role of COP1 in HCC pathogenesis.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 cell line, 2 protocol sets

Platform:

GPL6883

Series:

GSE21955

22 Samples

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(Submitter supplied) Development of targeted therapeutics for hepatocellular carcinoma (HCC) remains a major challenge. We have previously demonstrated that constitutively photomorphogenic 1 (COP1), which regulates p53 activity by ubiquitination, is frequently overexpressed in human HCC. Here we examined whether molecular targeting of COP1 by small interfering (si) RNA can affect the course of HCC progression. COP1-1 was selected as the most effective target siRNAs in terms of growth inhibition and apoptotic induction in several HCC cell lines. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4831

Platform:

GPL6883

22 Samples

Download data: TXT

(Submitter supplied) Development of targeted therapy for hepatocellular carcinoma (HCC) remains a major challenge. We have recently identified an elevated expression of the fifth subunit of COP9 signalosome (CSN5) in early HCC as compared to dysplastic stage. In the present study, we explored the possibility of CSN5 being a potential therapeutic target for HCC. We demonstrate that CSN5 knockdown by small interfering (si) RNA caused a block in cell proliferation and cell cycle progression, and induced apoptosis of HCC cells in vitro. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5210

Platform:

GPL6883

16 Samples

Download data: TXT

Analysis of Huh7 and HepG2 hepatocellular carcinoma (HCC) cells depleted for CSN5, the fifth subunit of the COP9 signalosome. CSN5 expression is elevated in early HCC. Result provide insight into the role of CSN5 in the pathogenesis of HCC.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 cell line, 2 protocol sets

Platform:

GPL6883

Series:

GSE26485

16 Samples

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(Submitter supplied) In order to assess the physiological role of Cop1 in vivo we generated mice that do no longer express the protein. Cop1KO mice die at around E10.5 of embryonic development. In order to gain insights into the molecular mechanisms that cause the embryonic death we compared the genome-wide gene expression profile of E9.5 wild-tytpe and Cop1-null embryos. The data do not support a role for Cop1 in the regulation of the p53 pathway in vivo and highlight a role for Cop1 in cardiovascular development and/or angiogenesis. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

4 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

14 Samples

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(Submitter supplied) MCF7 is an epithelial cell line isolated from the breast tissue of a patient with metastatic adenocarcinoma. Growth factors are well-known stimulators of glycometabolism. Whether glucose autoregulates its own metabolism remains poorly understood. To probe this, we designed the glucose-refeeding experiment in MCF7 for RNAseq analysis.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

8 Samples

Download data: CSV

(Submitter supplied) MCF7 is an epithelial cell line isolated from the breast tissue of a patient with metastatic adenocarcinoma. Whether glucose autoregulates its own metabolism remains poorly understood. To probe this, we performed RNA-Seq analysis on MCF7 breast cancer cells cultured with/without short-term glucose withdrawal.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

6 Samples

Download data: CSV