GEO DataSets

Analysis of well-differentiated primary lung bronchial epithelial cells 36 hs after infection with various H1N1 influenza isolates: seasonal H1N1 BN/59, pandemic H1N1 KY/136 and KY/180. Results provide insight into the molecular basis of host responses to different H1N1 Influenza isolates.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 4 infection sets

Platform:

GPL570

Series:

GSE48466

12 Samples

Download data: CEL

(Submitter supplied) We used microarrays to compare the gene expression profiles of different H1N1 isolates (seasonal and pandemic) in lung epithelial cells in vitro.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4855

Platform:

GPL570

12 Samples

Download data: CEL

(Submitter supplied) Pandemic influenza H1N1 (pdmH1N1) virus causes mild disease in humans but occasionally leads to severe complications and even death, especially in those who are pregnant or have underlying disease. Cytokine responses induced by pdmH1N1 viruses in vitro are comparable to other seasonal influenza viruses, suggesting the cytokine dysregulation as seen in H5N1 infection is not a feature of the pdmH1N1 virus. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4239

Platform:

GPL6244

9 Samples

Download data: CEL, CHP

Analysis of type I-like alveolar epithelial cells infected with pandemic influenza H1N1 (pdmH1N1) or seasonal H1N1. Type I alveolar epithelial cells are a key target cell in pdmH1N1 pneumonia. Results provide insight into molecular mechanisms underlying host responses induced by H1N1 infections.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 3 individual, 3 infection sets

Platform:

GPL6244

Series:

GSE24533

9 Samples

Download data: CEL, CHP

(Submitter supplied) Human disease caused by highly pathogenic avian influenza (HPAI) H5N1 can lead to a rapidly progressive viral pneumonia leading to acute respiratory distress syndrome. There is increasing evidence suggests a role for virus-induced cytokine dysregulation in contributing to the pathogenesis of human H5N1 disease. The key target cells for the virus in the lung are the alveolar epithelium and alveolar macrophages, and previous data has shown that compared to seasonal human influenza viruses, equivalent infecting doses of H5N1 viruses markedly up-regulate pro-inflammatory cytokines in both primary cell types in vitro. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3595

Platform:

GPL6244

27 Samples

Download data: CEL, CHP

Analysis of macrophages at 1, 3, and 6 hours post-infection with H1N1 or H5N1 viruses in vitro. The avian H5N1 virus is highly pathogenic, while the swine H1N1 virus is less so. Alveolar macrophages are targets of H5N1. Results provide insight into the host response to H1N1 and H5N1 infections.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 3 individual, 3 infection, 3 time sets

Platform:

GPL6244

Series:

GSE18816

27 Samples

Download data: CEL, CHP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

240 Samples

Download data

(Submitter supplied) An 8 hours timecourse was performed with human DCs infected either with A/California/7/2009 and A/Brevig Mission/1/1918 (pandemic) or A/New Caledonia/20/99 and A/Texas/36/91 seosonal.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

120 Samples

Download data: TXT

(Submitter supplied) An 8 hours timecourse was performed with human DCs infected either with A/California/7/2009 and A/Brevig Mission/1/1918 (pandemic) or A/New Caledonia/20/99 and A/Texas/36/91 seosonal.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

120 Samples

Download data: TXT

(Submitter supplied) Influenza A virus (IAV) is a human respiratory pathogen that causes yearly global epidemics, and sporadic pandemics due to human adaptation of pathogenic strains. Efficient replication of IAV in different species is, in part, dictated by its ability to exploit the genetic environment of the host cell. To investigate IAV tropism in human cells, we evaluated the replication of IAV strains in a diverse subset of epithelial cell lines. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20301

8 Samples

Download data: TXT

(Submitter supplied) Airway epithelial cells and macrophages differ markedly in their responses to influenza A virus (IAV) infection. To investigate transcriptional responses underlying these differences, purified subsets of type II airway epithelial cells (ATII) and alveolar macrophages (AM) recovered from the lungs of mock- or IAV-infected mice were subjected to RNA sequencing. In the absence of infection, AM predominantly expressed genes related to immunity whereas ATII expressed genes consistent with their physiological roles in the lung. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

96 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array; Expression profiling by high throughput sequencing

Platforms:

GPL17077 GPL18573

124 Samples

Download data: TXT

(Submitter supplied) The host innate immune response to influenza virus is a key determinant of pathogenic outcomes and long-term protective responses against subsequent exposures. Comparison of the transcriptional profiles obtained 24 and 36 hrs post-infection showed that the magnitude of gene expression was greater in LAIV infected cells relative to that observed in WT infected cells. Functional enrichment analysis revealed that the antiviral and inflammatory responses was largely driven by type III IFN induction in both WT and LAIV infected cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17077

118 Samples

Download data: TXT

(Submitter supplied) The host innate immune response to influenza virus is a key determinant of pathogenic outcomes and long-term protective responses against subsequent exposures. Comparison of the transcriptional profiles obtained 24 and 36 hrs post-infection showed that the magnitude of gene expression was greater in LAIV infected cells relative to that observed in WT infected cells. Functional enrichment analysis revealed that the antiviral and inflammatory responses was largely driven by type III IFN induction in both WT and LAIV infected cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

6 Samples

Download data: TXT

(Submitter supplied) A GFP-expressing recombinant A/Puerto Rico/8/1934 influenza virus was used to infect C57BL/6 wild type mice and on day 3 post infection, lung alveolar epithelial cells (AEC) were isolated and sorted based on GFP expression. GFP+ AEC represent the infected AEC and GFP- AEC represent the bystander AEC. AEC were also sorted from uninfected mice to serve as controls.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

13 Samples

Download data: TXT

(Submitter supplied) Influenza A virus has a broad cellular tropism in the respiratory tract. Infected epithelial cells sense the infection and initiate an antiviral response. To define the antiviral response at the earliest stages of infection we used two different single cycle replication reporter viruses. These tools demonstrated heterogeneity in virus replication levels in vivo. Transcriptional profiling demonstrated tiers of interferon stimulated gene responses that were dependent on the magnitude of virus replication. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

39 Samples

Download data: TXT

(Submitter supplied) We used two different influenza viruses lacking either PB1 or HA to differentiate the antiviral responses generated from primary transcription and full replication, respectively.

Organism:

Homo sapiens; Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL24247 GPL24676 GPL17021

184 Samples

Download data: TXT

(Submitter supplied) Critical disease caused by the new 2009 pandemic influenza virus (nvH1N1) is a challenge for physicians and scientist. As evidenced in SARS and H5N1, the development of an effective immune response plays a key role to overcome viral diseases. We studied host`s gene expression signatures, cytokine and antibody responses along the first week of hospitalization in 19 critically ill patients with primary nvH1N1 pneumonia and two degrees of respiratory involvement. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6102

40 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platforms:

GPL16847 GPL14550

12 Samples

Download data: TXT

(Submitter supplied) 8h and 24h after A/WSN/33 infection (MOI 1) ncRNA profiling was performed A growing body of evidence suggests gene regulatory functions for the majority of non-protein-coding RNAs (ncRNAs). Besides small RNAs (sRNAs), the diverse class of long ncRNAs (lncRNAs) recently came into focus of research. So far, the relevance of lncRNAs in infection processes remains elusive. Here, we report the differential expression of several classes of lncRNAs during influenza A virus (IAV) infection in human lung epithelial cells.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL16847

4 Samples

Download data: TXT