GEO DataSets

Analysis of bronchial epithelial cells from patients with severe asthma. Results provide insight into the molecular mechanisms underlying the development of severe asthma.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 3 disease state, 2 gender sets

Platform:

GPL6480

Series:

GSE43696

108 Samples

Download data: TXT

(Submitter supplied) Identification and characterization of gene expression using Next Generation Sequencing (NGS) of mRNA sequence from bronchial epithelial cells (BEC) obtained from asthma patients with varying clinical disease severity

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

67 Samples

Download data: TXT

(Submitter supplied) Bronchial Epithelial Cells were isolated processed as described (Chu et al., 2002 and Zhao et al., 2011). The objective of the study was to identify differentially expressed genes between normal control (NC), mild-moderate asmathic (notSA) and severe asthmatic (SA) patients. For demographics data, contact Dr. Sally Wenzel (wenzelse@upmc.edu)

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

104 Samples

Download data: TXT

(Submitter supplied) Bronchial Epithelial Cells were isolated processed as described (Chu et al., 2002 and Zhao et al., 2011). The objective of the study was to identify differentially expressed genes between normal control (NC), mild-moderate asmathic (notSA) and severe asthmatic (SA) patients. For demographics data, contact Dr.Sally Wenzel (wenzelse@upmc.edu)

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

155 Samples

Download data: TXT

(Submitter supplied) Bronchial Epithelial Cells were isolated processed as described (Chu et al., 2002 and Zhao et al., 2011). The objective of the study was to identify differentially expressed genes between normal control (NC), mild-moderate asthmatic (MMA) and severe asthmatic (SA) patients.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5037

Platform:

GPL6480

108 Samples

Download data: TXT

(Submitter supplied) Bronchial Epithelial Cells were isolated processed as described (Chu et al., 2002 and Zhao et al., 2011). The objective of the study was to identify differentially expressed genes between normal control (NC), mild-moderate asmathic (notSA) and severe asthmatic (SA) patients. For demographics data, contact Dr.Sally Wenzel (wenzelse@upmc.edu)

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4133

154 Samples

Download data: TXT

(Submitter supplied) Allergic asthmatic, allergy only, asthma only (no allergy), and non-allergic non-asthmatic (control) subjects underwent bronchoscopy with instillation of saline, lipopolysaccharide (LPS), and house dust mite antigen in separate subsegmental bronchi. Airway epithelial cells were collected four hours later (three samples per subject). RNA was extracted from these cells for microarray analysis. Keywords: gene expression arrays (two-dye: sample against common "universal" reference RNA)

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1708

250 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array; Methylation profiling by array

Platforms:

GPL21145 GPL28226

548 Samples

Download data: IDAT

(Submitter supplied) We generated genome-wide methylation data from primary cultured airway smooth muscle cells (ASMCs) exposed to IL-13, IL-17, IL-13+IL-17, and vehicle. This data was generated in combination with genome-wide expression data from the same individuals.

Organism:

Homo sapiens

Type:

Methylation profiling by array

Platform:

GPL21145

280 Samples

Download data: CSV, IDAT

(Submitter supplied) We generated genome-wide expression data from primary cultured airway smooth muscle cells (ASMCs) exposed to IL-13, IL-17, IL-13+IL-17, and vehicle. This data was generated in combination with genome-wide methylation data from the same individuals.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL28226

268 Samples

Download data: CSV

(Submitter supplied) Background: Although several studies link high levels of IL-6 and soluble IL-6 receptor (sIL-6R) with asthma severity and decreased lung function, the role of IL-6 trans-signaling (IL-6TS) in asthma is unclear. Objective: To explore the association between epithelial IL-6TS pathway activation and molecular and clinical phenotypes in asthma. Methods: Primary human bronchial epithelial cell (HBEC) air-liquid interface (ALI) cultures were stimulated with IL-6 and sIL-6R to establish an IL-6TS gene signature. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

24 Samples

Download data: TXT

(Submitter supplied) Next-generation sequencing of human BAL cells obtained from human patients with differing degrees of asthma in a search for variations in gene expressions.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

39 Samples

Download data: TXT

(Submitter supplied) This study identifies differentially expression genes in the sputum of people with eosinophilic, neutrophilic and paucigranulocytic asthma. A selection of markers identified using this microarray were further validated using qPCR on a wider sample set.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6104

47 Samples

Download data: TXT

(Submitter supplied) Severe asthma is a collection of disease entities with varying pathophysiological characteristics (7) that result in symptoms of cough, wheeze and breathlessness, with frequent exacerbations. To address the problem of phenotypic difference and heterogeneity, the Unbiased Biomarkers for the Prediction of Respiratory Disease Outcomes (U-BIOPRED) project was set up as a public-private partnership within the framework of the Innovative Medicines Initiative (IMI), engaging academia, the pharmaceutical industry and patient groups. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13158

498 Samples

Download data: CEL

(Submitter supplied) Interleukin (IL)-17 plays an important and protective role in host defence and has been demonstrated to orchestrate airway inflammation by cooperating with and inducing proinflammatory cytokines. Mircoarrays were used to identify immediate-early/ primary response IL-17A-dependent gene transcripts in primary human bronchial ASM cells from mild asthmatic and healthy individuals.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4803

Platform:

GPL6244

12 Samples

Download data: CEL

Analysis of airway smooth muscle cells (ASM) from mild asthmatics after a 2hr interleukin-17A (IL-17A) treatment. IL-17A orchestrates airway inflammation by cooperating with and inducing proinflammatory cytokines. Results provide insight into immediate-early/primary response gene targets of IL-17A.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent, 2 disease state sets

Platform:

GPL6244

Series:

GSE35643

12 Samples

Download data: CEL

(Submitter supplied) Evaluation of the airway transcriptome may reveal patterns of gene expression that are associated with clinical phenotypes of asthma. To define transcriptomic endotypes of asthma (TEA) we analyzed gene expression in induced sputum that correlate with phenotypes of disease. Gene expression was measured in sputum of subjects with asthma using Affymetrix HuGene ST 1.0 microarrays. Unsupervised clustering analysis of genes identified TEA clusters. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

112 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

59 Samples

Download data: CEL

(Submitter supplied) Background: In asthma, airway epithelium remodeling can already be detected during childhood, and epithelial cells are more susceptible to virus and oxidative stress. Their exact role in natural history and severity of children allergic respiratory disease remains however surprisingly unexplored. Aim: To analyze dysfunctions of epithelium in dust mite allergic respiratory disease (rhinitis ± asthma) in children. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

38 Samples

Download data: CEL

(Submitter supplied) Background: In asthma, airway epithelium remodeling can already be detected during childhood, and epithelial cells are more susceptible to virus and oxidative stress. Their exact role in natural history and severity of children allergic respiratory disease remains however surprisingly unexplored. Aim: To analyze dysfunctions of epithelium in dust mite allergic respiratory disease (rhinitis ± asthma) in children. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

21 Samples

Download data: CEL