GEO DataSets

Analysis of intact papillae isolated from male occipital scalp skin, 2D cultures of dermal papilla (DP) cells (passage 1, 3, 5) and 3D spheroid cultures of DP cells. Results provide insight into molecular mechanisms underlying spatial environment influences regulating dermal papilla cell induction.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 cell type, 3 individual, 5 other sets

Platform:

GPL570

Series:

GSE44765

18 Samples

Download data: CEL

(Submitter supplied) Dermal papilla cells isolated from the human hair follicle are capable of inducing hair growth in recipient epithelia. However, demonstrating disparity from rodent dermal papilla, human cells lose this inductive competance immediately upon growth in culture under normal growth conditions. We grew dermal papilla cells in hanging drop cultures that are morphologically akin to intact dermal papilla, and found that by enhancing the environment for aggregation, we could restore the inductive capacity of human dermal papilla cells in culture. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5296

Platform:

GPL570

18 Samples

Download data: CEL

(Submitter supplied) Perform bulk RNA-seq in two groups of samples. Group 1 (sample 1 to 12) , Four dermal components in Early Growth phase feather follicle. Group 2 (sample 13 to 21), Pulp in different developmental stages.

Organism:

Gallus gallus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19787

21 Samples

Download data: CSV

(Submitter supplied) Different types of hair follicles can be found in the skin of mice. It is believed that the signals that control hair follicle differentiation arise from cells in a structure called the dermal papilla. Understanding the nature of those signals is of interest for the biology of the normal tissue. We have developed a technique for isolation of dermal cells by enzymatic digestion of intact skin. We have identified two subpopulations of cells that can be separated by FACS. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

9 Samples

Download data: CEL, CHP

(Submitter supplied) Gene expression profiling reveals a potential higher effect of minoxdil and TCQA in stimulating hair growth in 3D cultered dermal papilla cells. HFDPCs were human primary cells line, treated with 0,1 µM minoxidil and 10 µM TCQA for 48 h. Microarray gene expression profiling was conducted for three biological replicates

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13667

5 Samples

Download data: CEL, TXT

(Submitter supplied) Gene expression profiling reveals a potential effect of minoxdil and TCQA in stimulating hair growth in 2D cultered dermal papilla cells. HFDPCs were human primary cells line, treated with 0,1 µM minoxidil and 10 µM TCQA for 48 h. Microarray gene expression profiling was conducted for three biological replicates

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13667

4 Samples

Download data: CEL, TXT

(Submitter supplied) The dermal papilla (DP) of the hair follicle plays crucial roles in the hair follcie morphogenesis and cycling. Thus, the elucication of human DP molecular signature is of great interest. DP cell culture by conventional method impairs intrinsic properties of DP cells. Isolatoion of human DP is hampered by the lack of specific cell surface markers. Thus, it still depends on manual microdissection, with which the removal of minor contamination is unfeasible. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL571

16 Samples

Download data: CEL

(Submitter supplied) Fgf20 is expressed from the hair follicle placode and is required for development of the dermal condensate. Here we used RNAseq to identify the immediate transcriptional targets of FGF20 in Fgf20-/- dermis at E13.5, a timepoint immediately prior to dermal condensate initiation.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

10 Samples

Download data: TXT

(Submitter supplied) Tissue homeostasis requires the balance of growth by cell production and regression through cellular loss. In the hair cycle during follicle regression, the niche traverses the skin through an unknown mechanism to reach the stem cells and prime regeneration. Here, by cell specific ablation and intravital imaging in live mice, we identify the follicle-lining dermal sheath as the key driver of tissue regression and niche relocation via smooth muscle contractile machinery that generates centripetal constriction force. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

12 Samples

Download data: XLSX

(Submitter supplied) Gene expression profiling reveals a potential role of Orange cells (BT-OC) in stimulating hair growth in dermal papilla cells. HFDPCs were human primary cells line, treated with 1:2000 Orange cells (BT-OC) for 48 h. Microarray gene expression profiling was conducted for three biological replicates

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13667

6 Samples

Download data: CEL, CHP, TXT

(Submitter supplied) Gene expression profiling reveals a potential role of Green cells (BT-GC) in stimulating hair growth in dermal papilla cells. HFDPCs were human primary cells line, treated with 1:2000 Green cells (BT-GC) for 48 h. Microarray gene expression profiling was conducted for three biological replicates

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13667

6 Samples

Download data: CEL, CHP, TXT

(Submitter supplied) The formation of tissue patterns is critical for organ functions in development and regeneration. To advance the use of organoids for organ regeneration, we learn principles that govern the skin organoids to regenerate hairs. We show the formation of epidermal-dermal coupled cysts function as competent morphogenetic units (CMU) which harbor the ability of skin organoids to regenerate. ScRNA-sequencing shows the emergence of cell types and new cell interactions during CMU formation. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

5 Samples

Download data: H5

(Submitter supplied) Organoid formation is critical to the progress of regenerative medicine. We use the formation of skin organoids from dissociated cells to study the self-organization process. Here we live-image the process which reveals an unexpectedly complex morphogenetic process: dissociated cells – aggregates – polarized cysts – fused cysts– planar skin – hair placodes. Transcriptome profiling and functional testing show orderly expression peaks of adhesion molecules, insulin-like growth factors, Wnts, extracellular matrix molecules, and matrix metalloproteinases are crucial to the sequential morphological transitions. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL13112 GPL19057

22 Samples

Download data: TXT