GEO DataSets

Analysis of whole colon tissue two weeks after C. rodentium infection of IL-10-deficient C57BL/6 females. C. rodentium is a mucosal pathogen that colonizes the colon causing transient mucosal inflammation. Results provide insight into the role of IL-10 in the colonic response to infection.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation, 2 infection sets

Platform:

GPL7202

Series:

GSE55812

4 Samples

Download data: TXT

(Submitter supplied) To further understand immune mechanims involved in regulating intestinal inflammation, we employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential of regulating inflammation in the absence of IL-10. Whole colon tissue from IL-10-deficient and C57BL/6 (wild-type) mice was collected 2 weeks after Citrobacter rodentium infection and from uninfected controls. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5629

Platform:

GPL7202

4 Samples

Download data: TXT

(Submitter supplied) Profiling of a total of 34,790 genes revealed a wide range of expression changes during the course of C. rodentium infection in murine colon. The majority of changes were observed during weeks 1 and 2, while relatively fewer changes were seen at week 3. Interestingly, chemokines made up 20% of the top twenty upregulated genes.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2897

4 Samples

Download data: XLS

(Submitter supplied) Infection by attaching and effacing (A/E) pathogens poses a serious threat to public health, as was highlighted by the recent outbreak of E. coli O157:H7 infection in the United States. Here, by using a murine A/E pathogen, Citrobacter rodentium, we demonstrate that C. rodentium infection is lethal to IL-22-/- mice within two weeks. IL-22, in the early phase of infection, is indispensable for preventing the invasion of bacteria through the intestinal epithelium, and thereby preventing systemic spread and mortality. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3226

Platform:

GPL2872

6 Samples

Download data: TXT

Analysis of C57BL/6 colon cultures treated with 10 ng/ml of interleukin-22 (IL-22) for 24 hours. IL-22, a member of the IL-10 family of cytokines, can induce a marked antimicrobial response in vitro. Results provide insight into the molecular basis of IL-22 induced host defense mechanisms.

Organism:

Mus musculus

Type:

Expression profiling by array, log10 ratio, 2 agent sets

Platform:

GPL2872

Series:

GSE10010

6 Samples

Download data: TXT

(Submitter supplied) We performed deep RNA sequencing and systematically analyzed the global gene expression profiles of C. rodentium-infected colon tissues from both susceptible and resistant congenic mice strains to determine the common transcriptomic responses to infection and the Rspo2-mediated dysregulated pathway signatures associated with the loss of disease tolerance.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

12 Samples

Download data: TXT

(Submitter supplied) IL-22 acts on epithelial cells and has been shown to induce tissue protective and wound healing responses in these cells. But it has recently been decribed that IL-22 exacerbates ileatis after infection with T. gondii. The goal of the study is to identify the IL-22-dependent genes during T. gondii infection in order to understand why IL-22 is pathogenic in this context.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

10 Samples

Download data: CEL

(Submitter supplied) miR-34a is a known tumor suppressor that targets cell proliferation and apoptosis genes. In this study, we have shown that miR-34a acts as a safeguard for the inflammatory stem cell niche and reparative regeneration by modulating both the immune and epithelial responses to infection and inflammation. We isolated CD4+ T cells and colon epithelial cells from miR-34a-/- and wildtype mice revealed various changes in gene expression.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

12 Samples

Download data: TXT

(Submitter supplied) This is to determine the regulation of gene expression in different T Cell population with short chain fatty acids. This will provide the roles of SCFAs in regulation of adaptive immunity and T-cell-mediated inflammation.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

14 Samples

Download data: CEL, CHP

(Submitter supplied) We report analysis of colonic transcriptome in gut epithelial cells specific deficient mouse (Il17ra villin cre +) and littermate control mouse (Il17ra villin cre -) infected with Citrobater rodentium (C. rodentium) for 10 days. We found RNA-seq data show Nox family genes and defensin genes are downregulated in Il17ra villin cre + mice. It is interestingly Tnfsf13 was also downregulated in Il17ra villin cre + mice. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21626

6 Samples

Download data: TXT

(Submitter supplied) We unveiled the disease relevance of the C. rodentium induced colitis model to IBD and demonstrated the protective role of the mucosal healing therapy IL-22.Fc in ameliorating the epithelial dysfunction

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21626

5 Samples

Download data: H5, MTX, TSV

(Submitter supplied) Three subsets of intestinal epithelial cells (IECs) (P9, Surface; P10, Large Crypt; P11, Small Crypt) were isolated from Naïve and Day 9 C.r.-infected Cntrl (Cre-) and CD4 cre+. Il22 floxed mice (CD4 cKO)

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

33 Samples

Download data: TXT

(Submitter supplied) Mice with and without IFNAR expression were treated with broad-spectrum antibiotics for more than 10 days, either infected with MNV or left uninfected and then treated with 3% DSS in drinking water for 6 days. Intestinal epithelial cells were isolated from the colon and sorted and RNA extracted for RNASeq analysis. We found that mice with IFNAR expression on IEC and infected with MNV had a gene expression signature of DNA repair similar that induced by IL-22 signaling.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

12 Samples

Download data: XLSX

(Submitter supplied) Overall hypothesis: RNAseq can be used to examine and compare the vitamin A (VA) effect, Infection effect caused by Citrobacter rodentium (C. rodentium), and the Interaction effect (VA status × C. rodentium infection) in mouse small intestine (SI) and colon, on the scale of global transcriptome. Methods: Vitamin A deficient (VAD) mice were generated by feeding VAD diet to the pregnant C57/BL6 dams and their post-weaning offspring. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

28 Samples

Download data: TXT

(Submitter supplied) scRNAseq of in vitro polarized Th17 and Th22 cells (Gpr65_WT versus KO), to compare metabolism gene expression in Gpr65_WT versus KO.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

8 Samples

Download data: MTX, TSV

(Submitter supplied) We identified two distinct subsets of absorptive epithelial cells in the mid-distal colons of mice that are regionally divided and differentially respond to IL-22 signals.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

6 Samples

Download data: MTX, TSV