GEO DataSets

(Submitter supplied) By comprehensive screening of long non-coding RNAs (lncRNAs) over mouse heart development, we have identified Tbx5 upstream antisense RNA (Tbx5ua). In order to understand its function, we produced Tbx5ua knock down ES cells by inserting triple bovine polyadenylation signal to the second exon of Tbx5ua. From the ES cells we made chimeric mouse embryos via tetraploid complementation assay. We conducted RNA-seq analysis on the WT and KD heart ventricles at E9.5 to further elucidate the lncRNA's molecular functions.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

6 Samples

Download data: TXT

(Submitter supplied) In order to compehensively determine long non-coding RNAs (lncRNAs) expressed in the mouse heart, we conducted RNA-seq on heart ventricles at three stages. We found that although lncRNAs are generally at a distance from protein-coding genes, cardiac transcription factor genes have lncRNAs in their proximity. Detailed examination revealed that many of these ln cRNAs were transcribed from the neighboring genes' promoter in a head-to-head divergent manner.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL13112

3 Samples

Download data: GTF, TXT