GEO DataSet Links for SRA (Select 5223484) - GEO DataSets

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Items: 2

Select item 3030400221.

wt/motAam [B2_S2]

Organism:: Tequatrovirus T4

Source name:: wt/motAam

Platform:: GPL24733
Series:: GSE111808

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Accession:: GSM3040022

ID:: 303040022

SRA Run Selector

Select item 2001118082.

The E. coli global regulator DksA attenuates transcription during T4 infection

(Submitter supplied) Purpose: We investigated how deletion of DksA or ppGpp, two E. coli global transcription regulators, affects T4 infection. Method: B606, B606 DdksA, and B606 ppGpp0 were grown at 37C to early/mid log phase (OD600 ~ 0.4) then infected with moi of 10 of either wt T4 or T4motAam and total RNA was isolated. 2.5 µg total RNA from each sample was treated with a Ribo-Zero rRNA Removal Kit (Gram-Negative Bacteria; Illumina San Diego, CA) to deplete rRNA. more...