GEO DataSets

(Submitter supplied) To investigate the rapid adaptation mechanism of Bacillus thuringiensis in an alkaline environment, we have employed whole genome microarray expression profiling as a discovery platform to identify the difference of gene expression between normal condition and alkaline condition.

Organism:

Bacillus thuringiensis

Type:

Expression profiling by array

Platform:

GPL17384

6 Samples

Download data: TXT

(Submitter supplied) We then performed gene expression profiling using data obtained from RNA-seq of 2 different cells at three time points.

Organism:

Bacillus thuringiensis serovar kurstaki str. HD73

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32767

18 Samples

Download data: TAB

(Submitter supplied) The formation of endospores has always been considered as the unique mode of survival and transmission of sporulating Firmicutes due to the exceptional resistance and persistence of this bacterial cell form. In this study, we investigated the behavior of a bacterial population in the late stages of an infection as well as the characteristics of the Spo- bacterial form in the B. thuringiensis/Galleria mellonella infection model. more...

Organism:

Bacillus thuringiensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32378

9 Samples

Download data: XLSX

(Submitter supplied) Transcriptome analyses after the transfer of pCER270-spec megaplasmid in B. cereus F4810-72 (previously cured for its plasmid), B. thuringiensis HD73, and B. weihenstephanensis KBAB6 strains. We determined differentially expressed genes in pCER270+ strains in comparison with pCER270- strains.

Organism:

Bacillus mycoides; Bacillus cereus; Bacillus thuringiensis

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL29834 GPL32378 GPL32936

36 Samples

Download data: TXT

(Submitter supplied) Spores of Bacillales and Clostridiales species contain 100s of different mRNAs, and their major function in Bacillus subtilis is to provide ribonucleotides for new RNA synthesis when spores germinate. In new work, RNA was isolated from spores of five Bacillales and one Clostridioides species and relative spore mRNA levels were determined by RNA-seq. Determination of RNA levels in single spores allowed calculation of RNA nt/spore, and assuming mRNA is 3% of spore RNA allowed calculation that only ~6% of spore mRNAs were present at ≥ 1/spore. more...

Organism:

Clostridioides difficile 630; Priestia megaterium QM B1551; Bacillus thuringiensis str. Al Hakam; Bacillus subtilis; Bacillus atrophaeus ATCC 9372; Geobacillus stearothermophilus ATCC 12980

Type:

Expression profiling by high throughput sequencing

6 related Platforms

12 Samples

Download data: TXT

(Submitter supplied) In planktonic and biofilm mimicking environments, the staphyloccocal transcriptome in t111+t13595 co-cultures showed significant upregulation of genes related to virulence factors contrary to those co-cultures with B. thuringiesis and K. oxytoca. In the biofilm polymicrobial environment, S. aureus transcriptome shows extensive downregulation of gene expression. The animal model co-infection with S. more...

Organism:

Klebsiella oxytoca; Staphylococcus aureus; Bacillus thuringiensis

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL28183 GPL28182 GPL20673

64 Samples

Download data: RPKM

(Submitter supplied) To understand which gene(s) are affected by 2-methylcitrate to lead to the delayed sporulation in ∆prpD, we extracted the total RNA from BMB171 and ∆prpD at 12 h for whole genome transcription analysis by RNA sequencing (RNA-Seq). Our RNA-seq data showed that transcriptions of almost all of the SigF regulon genes, as concluded from the B. subtilis regulon, were significantly down-regulated in ∆prpD compared with the wild type BMB171. more...

Organism:

Bacillus thuringiensis BMB171

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27747

4 Samples

Download data: XLSX

(Submitter supplied) RNA-seq has recently become a popular choice for gene expression studies. It is revolutionizing the fields of genomics and transcriptomics by enabling a wide range of applications along with high genome coverage and the ability to detect weakly expressed genes, which makes it an attractive alternative to microarrays. However, the field of RNA-Seq analysis is still evolving. In this study transcriptomic data from Bacillus thuringiensis strains ATCC10792 and CT43, grown in two Luria broth medium lots on four dates was obtained from Illumina High-Seq2000 and analyzed using DESeq2. more...

Organism:

Bacillus thuringiensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20724

2 Samples

Download data: XLSX

(Submitter supplied) Pathogenic species belonging to Bacillus cereus sensu lato group possess a high evolutionary advantage in the environment and in food matrices thanks to their capacity to survive as silent spores to harsh environmental insults and grow at relatively low temperatures. Ready to re-heat products are at severe risk for contamination by members of Bacillus cereus s.l. group if not stored at proper conditions. more...

Organism:

Bacillus cereus; [Bacillus thuringiensis] serovar konkukian str. 97-27

Type:

Expression profiling by array

Platform:

GPL19697

12 Samples

Download data: TXT

(Submitter supplied) To determine Sigma 54 (SigL) reglons in Bacillus thuringiensis HD73 strain, A sigLmutant, HD(ΔsigL::kan), was constructed with insertion of kanamycin resistance gene cassete. We have employed whole genome microarray expression profiling as a discovery platform to identify the difference of gene expression between mutant and wild-type strains.

Organism:

Bacillus thuringiensis

Type:

Expression profiling by array

Platform:

GPL17384

6 Samples

Download data: TXT

(Submitter supplied) We investigated the gene expression and metabolic regulatory mechanisms associated with the high-level accumulation of ICPs by performing the transcriptomics analysis of B. thuringiensis strain CT-43, using Illumina high throughout sequencing (RNA-seq) technique.

Organism:

Bacillus thuringiensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15828

4 Samples

Download data: TXT

(Submitter supplied) Analysis of genomic content of closely related Bacillus species. Refer to individual records for strain information. Refer to platform and individual sample records for experimental protocols. Keywords: other

Organism:

Bacillus anthracis; Bacillus cereus; Bacillus mycoides; Bacillus thuringiensis

Type:

Genome variation profiling by array

Platform:

GPL266

60 Samples

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(Submitter supplied) Combimatrix Bacillus cereus sensu latu group germination and outgrowth in food model Complete genome sequence of B. thuringiensis sv konkukian str. 97-27 (NCBI Reference Sequence: NC_005957.1) was chosen for its high homology with B. cereus s.l. UC10070, to design probes corresponding to 5,197 genes spotted in duplicates onto ElectraSenseH 12K microarrays chip. Protocol: Probes are directly synthesized on a 12k Combimatrix array by an in situ oligo synthesis system developed by Combimatrix Corporation (Mukilteo, USA) using the Combimatrix CustomArray synthesizer. more...

Organism:

[Bacillus thuringiensis] serovar konkukian str. 97-27

1 Series

12 Samples

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(Submitter supplied) Arrays of this design have barcodes that begin with 16037484 or 2537484. Orientation: Features are numbered numbered Left-to-Right, Top-to-Bottom as scanned by an Agilent scanner (barcode on the left, DNA on the back surface, scanned through the glass), matching the FeatureNum output from Agilent's Feature Extraction software. The ID column represents the Agilent Feature Extraction feature number. more...

Organism:

Bacillus thuringiensis

2 Series

12 Samples

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