GEO DataSets

(Submitter supplied) Comparison of Azotobacter vinelandii str. DJ transcriptomes using either ammonium or dinitrogen as sole nitrogen source. Transcriptomes from cells in early stages of nitrogenase derepression (10 min and 30 min) and at the peak of nitrogenase derepression (4 hours) were compared against the corresponding controls from cells using ammonium as nitrogen source. Kinetics of the nitrogen fixation (nif) genes expression profiles resemble those previously published (Poza-Carrion, 2013; doi: 10.1128/jb.00942-13).

Organism:

Azotobacter vinelandii DJ

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33825

6 Samples

Download data: TXT

(Submitter supplied) Biological nitrogen fixation, the microbial reduction of atmospheric nitrogen to bioavailable ammonia, represents both a major limitation on biological productivity and a highly desirable engineering target for synthetic biology. However, the engineering of nitrogen fixation requires an integrated understanding of how the gene regulatory dynamics of host diazotrophs respond across sequence-function space of its central catalytic metalloenzyme, nitrogenase. more...

Organism:

Azotobacter vinelandii DJ

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33461

6 Samples

Download data: TXT

(Submitter supplied) The transcriptional differences found during stationary-phase ammonium accumulation show a strong contrast between the deregulated (nifL disrupted) and wild-type strain, and to what was reported for the wild-type strain under exponential growth related to key processes involved in driving the process of nitrogen fixation in A. vinelandii. These results further illuminate a number of additional genes associated with siderophore synthesis, molybdate transfer and electron transfer that are likely associated with biological nitrogen fixation.

Organism:

Azotobacter vinelandii

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23257

15 Samples

Download data: TXT

(Submitter supplied) NimbleGen Serial No: 080201_HE_NTNU_Azo_vinelandii_expr_x4. A 4x72k array covering 5012 genes from Azotobacter vinelandii DJ. SEQ_IDs are based on the geneIDs from the A. vinelandii DJ genome annotation. The geneIDs of certain ORFs in the DJ genome were however reassigned after manufacture of the array, causing a discrepancy between the SEQ_ID and GENE_ID columns in the matrix worksheet. Protocol: High-density DNA array prepared with Maskless Array Synthesizer (MAS) technology. more...

Organism:

Azotobacter vinelandii DJ

Download data: NCD, NDF, NGD