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Items: 1 to 20 of 2464025

1.

Transcriptome (RNA-seq) of Huh7 cells non-infected (NI) or infected (Inf.) with the pathogen Listeria monocytogenes (strain EGD-e)

(Submitter supplied) Comparisons of gene expression profiles of human hepatocytes (Huh7) infected or not by the bacterium Listeria monocytogenes (strain EGD-e) for 72 hours and analysed by RNA-seq
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18573
6 Samples
Download data: TXT
Series
Accession:
GSE184729
ID:
200184729
2.

Novel temporal and spatial patterns of metastatic colonization from breast cancer rapid-autopsy tumor biopsies

(Submitter supplied) We collected 42 samples from a breast cancer patients including 28 metastatic tumors (which also had DNA samples) and 14 surrounding normal samples. We generated RNAseq data from these samples to study the differential expression of the metastatic tumors.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL11154
42 Samples
Download data: TXT
Series
Accession:
GSE184717
ID:
200184717
3.

Next Generation Sequencing Facilitates Quantitative Analysis of endothelial cells transfected with Ptbp1(wt), Ptbp1(T138A) or Ptbp1(T138E) plasmid Transcriptomes

(Submitter supplied) Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived endothelial cell transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL11154
9 Samples
Download data: XLSX
Series
Accession:
GSE184631
ID:
200184631
4.

Transcriptome analysis of genes that regulated by lnc-CXCL2-4-1

(Submitter supplied) To demonstrate the regulating function of lnc-CXCL2-4-1 in lung epithelial cells after viral infection, we performed transcriptome sequencing of wild-type and lnc-CXCL2-4-1-silenced A549 cells after influenza A virus (IAV) infection, and then analyzed differentially expressed genes.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
2 Samples
Download data
Series
Accession:
GSE179951
ID:
200179951
5.

Single-cell analysis of pathology in Klinefelter syndrome and idiopathic male infertility

(Submitter supplied) Klinefelter syndrome (KS), also known as 47,XXY,  is characterized by a distinct set of physiological abnormalities, commonly including infertility. The molecular basis for Klinefelter-related infertility is still unclear, largely due to the cellular complexity of the testis and the intricate endocrine and paracrine signaling that regulates spermatogenesis. Here, we demonstrate an analysis framework for dissecting human testis pathology that uses comparative analysis of single-cell RNA-sequencing data from the biopsies of 13 human donors. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL16791
7 Samples
Download data: TXT
Series
Accession:
GSE169062
ID:
200169062
6.

FlowCT for the analysis of large immunophenotypic datasets and biomarker discovery in cancer immunology

(Submitter supplied) Large-scale immune monitoring is becoming routinely used in clinical trials to identify determinants of treatment responsiveness, particularly to immunotherapies. Flow cytometry remains one of the most versatile and high throughput approaches for single-cell analysis; however, manual interpretation of multidimensional data poses a challenge to capture full cellular diversity and provide reproducible results. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18573
30 Samples
Download data: TXT
Series
Accession:
GSE166711
ID:
200166711
7.

miRNA changes after overexpression of LINC01126 in HepG2 cells

(Submitter supplied) To identify putative miRNA targets for LINC01126, we overexpressed LINC01126 in HepG2 cells and carried out a microarray analysis. A total of 66 miRNAs present in the array demonstrated 1.5-fold upregulation or downregulation of expression upon LINC01126 transfection.
Organism:
Homo sapiens
Type:
Non-coding RNA profiling by array
Platform:
GPL20712
2 Samples
Download data: TXT, XLS
Series
Accession:
GSE184745
ID:
200184745
8.

Expansion of Human Papillomavirus-Specific T Cells in Periphery and Cervix in a Therapeutic Vaccine Recipient Whose Cervical High-Grade Squamous Intraepithelial Lesion Regressed

(Submitter supplied) Single-cell TCR/transcriptome analysis for a peripheral blood lymphocytes (PBL) specimen was perform on a 10X Chromium instrument using a single-cell VDJ kit
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing; Other
Platform:
GPL21697
2 Samples
Download data: CSV, MTX, TSV
Series
Accession:
GSE184703
ID:
200184703
9.

Transcriptome (RNA-seq) of HepG2 cells non-infected (NI) or infected (Inf.) with the pathogen Listeria monocytogenes (strain EGD-e)

(Submitter supplied) Comparisons of gene expression profiles of human hepatocytes (HepG2) infected or not by the bacterium Listeria monocytogenes (strain EGD-e) for 72 hours and analysed by RNA-seq
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18573
6 Samples
Download data: TXT
Series
Accession:
GSE184697
ID:
200184697
10.

Human hepatocellular carcinoma RNAseq data

(Submitter supplied) This is a series of RNAseq data obtained from the predominant region of 29 treatment-naïve, surgically-resected human HCCs.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
29 Samples
Download data: TXT
Series
Accession:
GSE183406
ID:
200183406
11.

10X genomics single cell GEX and VDJ 5' sequencing of PBMC from Type 1 Diabetes patients treated with Treg therapy alone or plus low dose IL-2

(Submitter supplied) Background: A previous Phase I study showed that the infusion of autologous Treg cells expanded ex-vivo into recent onset Type 1 Diabetes (T1D) patients had an excellent safety profile, however, the majority of the infused Tregs could no longer be detected in the peripheral blood three months post-infusion (NCT01210664-Treg-T1D Trial). Interleukin-2 (IL-2) is a well-characterized cytokine that has been shown to enhance human Treg cell survival and expansion at low doses in vivo. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
84 Samples
Download data: H5AD
Series
Accession:
GSE178991
ID:
200178991
12.

Differentially polarized human monocyte-derived macrophages (M0, M1, M2/IL10, M2/IL4, TAM-like)

(Submitter supplied) In order to assess whether differences in the transcriptomic profile of human tumor-associated macrophages vs. alveolar macrophages from adjacent non-tumor-tissue (GSE162669) were caused by factors secreted by tumor cells, primary human monocyte-derived macrophages (MDMs) were polarized towards a TAM-like phenotype by cultivating them in tumor cell-conditioned medium. A genome-wide comparison by bulk RNA-Seq confirmed a high similarity of ex vivo TAMs and in vitro polarized TAM-like macrophages. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL16791
15 Samples
Download data: TXT
Series
Accession:
GSE162698
ID:
200162698
13.

Human tumor-associated macrophages from lung adenocarcinoma vs. alveolar macrophages from adjacent non-tumor tissue

(Submitter supplied) Human tumor-associated macrophages (TAMs) were isolated from lung adenocarcinoma tissues and compared with alveolar macrophages (AMs) obtained from adjacent non-tumor tissues. For details, see Hoppstädter et al., 2021 (doi:10.1016/j.ebiom.2021.103578).
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL16791
18 Samples
Download data: TXT
Series
Accession:
GSE162669
ID:
200162669
14.

Targeting EYA2 tyrosine phosphatase activity in glioblastoma stem cells induces mitotic catastrophe

(Submitter supplied) Glioblastoma ranks among the most lethal of primary brain malignancies, with glioblastoma stem cells (GSCs) at the apex of tumor cellular hierarchies. Here, to discover novel therapeutic GSC targets, we interrogated gene expression profiles from GSCs, differentiated glioblastoma cells (DGCs), and neural stem cells (NSCs), revealing EYA2 as preferentially expressed by GSCs. Targeting EYA2 impaired GSC maintenance and induced cell cycle arrest, apoptosis, and loss of self-renewal. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL20301
6 Samples
Download data: XLSX
Series
Accession:
GSE135306
ID:
200135306
15.

ARID3a is Required for Erythrocyte Lineage Differentiation of Hemin Stimulated Human K562 Cells

(Submitter supplied) Inhibition of ARID3a by shRNA in hemin-induced K562 cells results in significant decrease of erythroid specific lineage factors and alpha-globin genes.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL18573
56 Samples
Download data: TXT
Series
Accession:
GSE131649
ID:
200131649
16.

The transcriptomes of PC3 cells with or without POH1 interference

(Submitter supplied) Total RNA was obtained from PC3 cells with or without POH1 interference to perform a High-throughput sequencing
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL20795
2 Samples
Download data: TXT
Series
Accession:
GSE120481
ID:
200120481
17.

Gene expression data from trastuzumab-hypersensitive clones

(Submitter supplied) Genes involved in trastuzumab response were identified using transcriptomic data from cells hypersensitive to trastuzumab derived from BT474.
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL16686
3 Samples
Download data: CEL, CHP
Series
Accession:
GSE184655
ID:
200184655
18.

RNA expression and m5c modification in NSUN2 kncok down CaSki cell

(Submitter supplied) 5-methylcytosine (m5C) modification ubiquitously occurs on mammalian mRNAs and plays important roles in multiple biological processes. Currently, the role of m5C in cancer initiation and progression is gaining more and more research attention, but the detailed mechanism remains unclear. As an m5C methyltransferase with unique mRNA catalytic activity, NSUN2 was found to be highly expressed in multiple cancers including gastric, bladder, gallbladder, and breast cancers, suggesting NSUN2 might exert oncogenic properties through affecting m5C levels in cancer cells. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing; Other
Platform:
GPL11154
6 Samples
Download data: XLSX
Series
Accession:
GSE184621
ID:
200184621
19.

Impact of ADA2 on human macrophages

(Submitter supplied) This study aims to understand the impact of adenosine deaminase 2 (ADA2) on human monocyte-derived macrophages
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL11154
6 Samples
Download data: TXT
Series
Accession:
GSE184572
ID:
200184572
20.

Human circulating T cells with skin tissue-resident phenotype (cTRM) display Th2-like inflammatory profile

(Submitter supplied) To investigate the transcriptional profile of human circulating T cells with skin tissue-resident memory phenotype (cTRM) in GVHD we performed bulk RNA sequencing from SMART-seq2 of sorted human cTRM (CD3+ CD4+ CLA+ CD103+) and conventional T cells (CD3+ CD4+ CD103-), and single cell RNA sequencing from 10X Genomics of sorted human CD45+, both from isolated blood PBMCs of patients with active acute GVHD.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
9 Samples
Download data: TXT
Series
Accession:
GSE183212
ID:
200183212
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