Nkx2-5 NK2 homeobox 5 [ Rattus norvegicus (Norway rat) ]

Gene ID: 114109, updated on 30-Mar-2024

Summary

Official Symbol: Nkx2-5provided by RGD
Official Full Name: NK2 homeobox 5provided by RGD
Primary source: RGD:620520
See related: Ensembl:ENSRNOG00000020747 AllianceGenome:RGD:620520
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Csx; Nkx2.5
Summary: Enables several functions, including DNA-binding transcription factor activity; histone deacetylase binding activity; and identical protein binding activity. Involved in several processes, including cellular response to hydrogen peroxide; negative regulation of transcription by RNA polymerase II; and response to estradiol. Located in nucleus. Biomarker of pulmonary fibrosis. Human ortholog(s) of this gene implicated in aortic valve disease 2; atrioventricular block; congenital heart disease (multiple); and congenital nongoitrous hypothyroidism 5. Orthologous to human NKX2-5 (NK2 homeobox 5). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Restricted expression toward (RPKM 179.0) See more
Orthologs: human mouse all
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Genomic context

Location:: 10q12

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	10	NC_086028.1 (16844888..16851458)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	10	NC_051345.1 (16340428..16347004)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	10	NC_005109.4 (16635989..16638758)

Chromosome 10 - NC_086028.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Nkx2-5 Regulates the Proliferation and Migration of H9c2 Cells.
Title: Nkx2-5 Regulates the Proliferation and Migration of H9c2 Cells.
Homocysteine downregulates cardiac homeobox transcription factor NKX2.5 via IGFBP5.
Title: Homocysteine downregulates cardiac homeobox transcription factor NKX2.5 via IGFBP5.
The authors findings suggest that Nkx2.5, as a transcription factor, may be a regulatory element connecting different signaling pathways in response to oxidative stress and that reduced Nsd1 (Nuclear receptor-binding SET domain-containing protein 1) expression could lead to the alteration of global H3K36 and H4K20 methylation, influencing cardiac development.
Title: Role of Nkx2.5 in H(2)O(2)-induced Nsd1 suppression.
The data indicated that inhibition of Smad4 diminished both AdBMP2 induced and basal histone acetylation levels in the promoter regions of GATA4 and Nkx2.5.
Title: Smad4 mediated BMP2 signal is essential for the regulation of GATA4 and Nkx2.5 by affecting the histone H3 acetylation in H9c2 cells.
SIRT1 acts as a direct transcriptional target of Nkx2.5 that maintains cardiomyocyte homeostasis and survival.
Title: SIRT1 mediates the protective function of Nkx2.5 during stress in cardiomyocytes.
pacemaking cells originate from Nkx2.5 positive cardiac progenitor cells present in embryonic heart tubes
Title: Endothelin-induced differentiation of Nkx2.5⁺ cardiac progenitor cells into pacemaking cells.
GATA-4 and Nkx2. 5 gene expression increased gradually in the course of mesenchymal stem cells differentiation into cardiomyocytes.
Title: [The expression of GATA-4 and Nkx2.5 gene in the transformation of Rattus mesenchymal stem cells into cardiomyocytes].
novel function for Nkx2.5 as a repressor of alpha-SMA gene transcription, which may be of homeostatic significance as a means of suppressing myofibroblast differentiation
Title: Nkx2.5/Csx represses myofibroblast differentiation.
p300 acts as an Nkx2.5 cofactor and facilitates increased Nkx2.5 activity by relieving the conformational impediment of its inhibitory C-terminal domain.
Title: Carboxyl terminus of Nkx2.5 impairs its interaction with p300.
Nkx2.5 regulates expression of Ca2+ channels in neonatal heart.
Title: Transcription factors Csx/Nkx2.5 and GATA4 distinctly regulate expression of Ca2+ channels in neonatal rat heart.

Submit: New GeneRIF Correction See all GeneRIFs (11)

General gene information

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Markers

UniSTS:472868 (e-PCR)
- UniSTS:472868

Nkx2-5 (e-PCR), detects polymorphism
- UniSTS:472868

UniSTS:463422 (e-PCR)
- UniSTS:463422

Nkx2-5 (e-PCR), detects polymorphism
- UniSTS:463422

Nkx2-5 (e-PCR), detects polymorphism
- UniSTS:527451

Nkx2-5 (e-PCR), detects polymorphism
- UniSTS:144024

Nkx2-5 (e-PCR), detects polymorphism
- UniSTS:144025

RH133759 (e-PCR)
- UniSTS:217042

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables DNA binding	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription activator activity	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription activator activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to DNA-binding transcription factor activity	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor activity	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	ISS Inferred from Sequence or Structural Similarity more info
enables RNA polymerase II-specific DNA-binding transcription factor binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II-specific DNA-binding transcription factor binding	ISO Inferred from Sequence Orthology more info
enables chromatin binding	IEA Inferred from Electronic Annotation more info
enables chromatin binding	ISO Inferred from Sequence Orthology more info
enables histone deacetylase binding	IPI Inferred from Physical Interaction more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IEA Inferred from Electronic Annotation more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables sequence-specific double-stranded DNA binding	ISO Inferred from Sequence Orthology more info
enables transcription cis-regulatory region binding	ISO Inferred from Sequence Orthology more info
enables transcription cis-regulatory region binding	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in Purkinje myocyte differentiation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within Purkinje myocyte differentiation	ISO Inferred from Sequence Orthology more info
involved_in adult heart development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within adult heart development	ISO Inferred from Sequence Orthology more info
involved_in adult heart development	ISO Inferred from Sequence Orthology more info
acts_upstream_of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in apoptotic process involved in heart morphogenesis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within apoptotic process involved in heart morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in atrial cardiac muscle tissue development	IEA Inferred from Electronic Annotation more info
involved_in atrial cardiac muscle tissue development	ISO Inferred from Sequence Orthology more info
involved_in atrial septum morphogenesis	IEA Inferred from Electronic Annotation more info
involved_in atrial septum morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in atrioventricular node cell development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within atrioventricular node cell development	ISO Inferred from Sequence Orthology more info
involved_in atrioventricular node cell fate commitment	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within atrioventricular node cell fate commitment	ISO Inferred from Sequence Orthology more info
involved_in atrioventricular node development	ISO Inferred from Sequence Orthology more info
involved_in bundle of His development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within bundle of His development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cardiac conduction system development	ISO Inferred from Sequence Orthology more info
involved_in cardiac conduction system development	ISO Inferred from Sequence Orthology more info
involved_in cardiac muscle cell development	ISO Inferred from Sequence Orthology more info
involved_in cardiac muscle cell differentiation	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within cardiac muscle cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in cardiac muscle cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in cardiac muscle cell proliferation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cardiac muscle cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in cardiac muscle contraction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cardiac muscle contraction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cardiac muscle tissue development	ISO Inferred from Sequence Orthology more info
involved_in cardiac muscle tissue morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in cardiac septum morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in cardiac ventricle formation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cardiac ventricle formation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cardiac ventricle morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in cell differentiation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in cellular response to growth factor stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to hydrogen peroxide	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within embryonic heart tube development	ISO Inferred from Sequence Orthology more info
involved_in embryonic heart tube left/right pattern formation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within embryonic heart tube left/right pattern formation	ISO Inferred from Sequence Orthology more info
involved_in epithelial cell apoptotic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of epithelial cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in epithelial cell differentiation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within epithelial cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in epithelial cell proliferation	IEA Inferred from Electronic Annotation more info
acts_upstream_of epithelial cell proliferation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within heart contraction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within heart development	ISO Inferred from Sequence Orthology more info
involved_in heart development	ISO Inferred from Sequence Orthology more info
involved_in heart looping	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within heart looping	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within heart morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in heart morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in heart trabecula formation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within heart trabecula formation	ISO Inferred from Sequence Orthology more info
involved_in hemopoiesis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within hemopoiesis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of canonical Wnt signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of canonical Wnt signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cardiac muscle cell apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of cardiac muscle cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of epithelial cell apoptotic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of epithelial cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of myotube differentiation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of myotube differentiation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within outflow tract morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in outflow tract septum morphogenesis	IEA Inferred from Electronic Annotation more info
involved_in outflow tract septum morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in pharyngeal system development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within pharyngeal system development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cardioblast differentiation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cardioblast differentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of epithelial cell proliferation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of epithelial cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of gene expression	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of heart contraction	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of heart contraction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of neuron differentiation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of neuron differentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of sodium ion transport	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of sodium ion transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of transcription initiation by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of transcription initiation by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in proepicardium development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within proepicardium development	ISO Inferred from Sequence Orthology more info
involved_in pulmonary myocardium development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within pulmonary myocardium development	ISO Inferred from Sequence Orthology more info
involved_in regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in regulation of cardiac conduction	IEA Inferred from Electronic Annotation more info
involved_in regulation of cardiac conduction	ISO Inferred from Sequence Orthology more info
involved_in regulation of cardiac muscle cell proliferation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of cardiac muscle cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in regulation of cardiac muscle contraction	IEA Inferred from Electronic Annotation more info
involved_in regulation of cardiac muscle contraction	ISO Inferred from Sequence Orthology more info
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in response to estradiol	IEP Inferred from Expression Pattern more info	PubMed
involved_in right ventricular cardiac muscle tissue morphogenesis	IEA Inferred from Electronic Annotation more info
involved_in right ventricular cardiac muscle tissue morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in septum secundum development	IEA Inferred from Electronic Annotation more info
involved_in septum secundum development	ISO Inferred from Sequence Orthology more info
involved_in spleen development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within spleen development	ISO Inferred from Sequence Orthology more info
involved_in spleen development	ISO Inferred from Sequence Orthology more info
involved_in spleen development	ISS Inferred from Sequence or Structural Similarity more info
involved_in thyroid gland development	IEA Inferred from Electronic Annotation more info
involved_in thyroid gland development	ISO Inferred from Sequence Orthology more info
involved_in transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
acts_upstream_of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in vasculogenesis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within vasculogenesis	ISO Inferred from Sequence Orthology more info
involved_in ventricular cardiac myofibril assembly	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within ventricular cardiac myofibril assembly	ISO Inferred from Sequence Orthology more info
involved_in ventricular septum morphogenesis	IEA Inferred from Electronic Annotation more info
involved_in ventricular septum morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in ventricular trabecula myocardium morphogenesis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within ventricular trabecula myocardium morphogenesis	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of Nkx-2.5 complex	IEA Inferred from Electronic Annotation more info
part_of Nkx-2.5 complex	ISO Inferred from Sequence Orthology more info
part_of RNA polymerase II transcription regulator complex	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
part_of protein-DNA complex	IEA Inferred from Electronic Annotation more info
part_of protein-DNA complex	ISO Inferred from Sequence Orthology more info
part_of protein-DNA complex	ISS Inferred from Sequence or Structural Similarity more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	ISS Inferred from Sequence or Structural Similarity more info
part_of transcription regulator complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: homeobox protein Nkx-2.5

Names: homeobox protein NK-2 homolog E; rNKx-2.5

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_053651.2 → NP_446103.2 homeobox protein Nkx-2.5

Status: VALIDATED

Source sequence(s)

JAXUCZ010000010

UniProtKB/Swiss-Prot

O35767

UniProtKB/TrEMBL

A6HDD5, G3V8T2

Related

ENSRNOP00000028155.3, ENSRNOT00000028155.6

Conserved Domains (1) summary

pfam00046
Location:144 → 194

Homeobox; Homeobox domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086028.1 Reference GRCr8

Range

16844888..16851458

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039085022.2 → XP_038940950.1 homeobox protein Nkx-2.5 isoform X1

UniProtKB/Swiss-Prot

O35767

UniProtKB/TrEMBL

A6HDD5, G3V8T2

Conserved Domains (1) summary

pfam00046
Location:144 → 194

Homeobox; Homeobox domain