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Gne glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase [ Rattus norvegicus (Norway rat) ]

Gene ID: 114711, updated on 30-Mar-2024

Summary

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Official Symbol
Gneprovided by RGD
Official Full Name
glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinaseprovided by RGD
Primary source
RGD:69239
See related
Ensembl:ENSRNOG00000014365 AllianceGenome:RGD:69239
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as
Uae1
Summary
Enables N-acylmannosamine kinase activity and UDP-N-acetylglucosamine 2-epimerase activity. Predicted to be involved in N-acetylneuraminate biosynthetic process. Located in cytosol. Human ortholog(s) of this gene implicated in GNE myopathy and sialuria. Orthologous to human GNE (glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in Liver (RPKM 304.5), Spleen (RPKM 79.3) and 8 other tissues See more
Orthologs
human mouse all
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Genomic context

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Location:
5q22
Exon count:
15
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) 5 NC_086023.1 (63062953..63103320, complement)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 5 NC_051340.1 (58267189..58307396, complement)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 5 NC_005104.4 (59511738..59553421, complement)

Chromosome 5 - NC_086023.1Genomic Context describing neighboring genes Neighboring gene calicin Neighboring gene clathrin, light chain A Neighboring gene small ribosomal subunit protein eS12-like Neighboring gene ring finger protein 38

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Biochemical characterization of the M712T-mutation of the UDP-N-acetylglucosamine 2-epimerase/N-acetyl-mannosaminekinase in hereditary inclusion body myopathy. Weidemann W, et al. Neuromuscul Disord, 2011 Dec. PMID 21873062
  2. The key enzyme of sialic acid biosynthesis (GNE) promotes neurite outgrowth of PC12 cells. Kontou M, et al. Neuroreport, 2008 Aug 6. PMID 18628673
  3. Characterization of ligand binding to the bifunctional key enzyme in the sialic acid biosynthesis by NMR: I. Investigation of the UDP-GlcNAc 2-epimerase functionality. Blume A, et al. J Biol Chem, 2004 Dec 31. PMID 15498764
  4. Domain-specific characteristics of the bifunctional key enzyme of sialic acid biosynthesis, UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase. Blume A, et al. Biochem J, 2004 Dec 15. PMID 15330759, Free PMC Article
  5. Genetic dissection of increased urinary albumin excretion in the munich wistar frömter rat. Schulz A, et al. J Am Soc Nephrol, 2002 Nov. PMID 12397040

See all (18) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Variations in the mRNA expression level of UDP-GlcNAc epimerase/ManNAc kinase and neuraminidase 1 genes in organs of type 2 diabetic animals.
    Title: Variations in the mRNA expression level of UDP-GlcNAc epimerase/ManNAc kinase and neuraminidase 1 genes in organs of type 2 diabetic animals.
  2. M712T mutation of GNE gene does not affect GNE oligomerization but affects the solubility and posttranslational modification of the GNE protein.
    Title: Biochemical characterization of the M712T-mutation of the UDP-N-acetylglucosamine 2-epimerase/N-acetyl-mannosaminekinase in hereditary inclusion body myopathy.
  3. Data show that overexpression of GNE induces neurite outgrowth in PC12 cells, but does not lead to an increased biosynthesis of sialic acid.
    Title: The key enzyme of sialic acid biosynthesis (GNE) promotes neurite outgrowth of PC12 cells.
  4. UDP-GlcNAc 2-epimerase/ManNAc kinase expression is regulated on the transcriptional level by DNA methylation
    Title: Epigenetically mediated loss of UDP-GlcNAc 2-epimerase/ManNAc kinase expression in hyposialylated cell lines.
  5. analysis of ligand binding to UDP-GlcNAc 2-epimerase by NMR
    Title: Characterization of ligand binding to the bifunctional key enzyme in the sialic acid biosynthesis by NMR: I. Investigation of the UDP-GlcNAc 2-epimerase functionality.
  6. The collapsin response mediator protein 1 and the promyelocytic leukemia zinc finger protein interact with UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE).
    Title: The collapsin response mediator protein 1 (CRMP-1) and the promyelocytic leukemia zinc finger protein (PLZF) bind to UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE), the key enzyme of sialic acid biosynthesis.
  7. structures required for dimerization are localized within the kinase domain, and a potential trimerization site is possibly located in a region between the two domains
    Title: Domain-specific characteristics of the bifunctional key enzyme of sialic acid biosynthesis, UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase.
  8. analysis of oligomeric states of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase by biophysical methods
    Title: Evidence for dynamic interplay of different oligomeric states of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase by biophysical methods.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables N-acylmannosamine kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables N-acylmannosamine kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables N-acylmannosamine kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables N-acylmannosamine kinase activity ISO
Inferred from Sequence Orthology
more info
  
enables UDP-N-acetylglucosamine 2-epimerase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables UDP-N-acetylglucosamine 2-epimerase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables UDP-N-acetylglucosamine 2-epimerase activity IEA
Inferred from Electronic Annotation
more info
  
enables UDP-N-acetylglucosamine 2-epimerase activity ISO
Inferred from Sequence Orthology
more info
  
enables hydrolase activity, hydrolyzing O-glycosyl compounds IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in N-acetylglucosamine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in N-acetylneuraminate biosynthetic process IC
Inferred by Curator
more info
 PubMed 
involved_in N-acetylneuraminate biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in N-acetylneuraminate biosynthetic process ISO
Inferred from Sequence Orthology
more info
  
involved_in N-acetylneuraminate biosynthetic process TAS
Traceable Author Statement
more info
 PubMed 
involved_in UDP-N-acetylglucosamine metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in carbohydrate phosphorylation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
Names
UDP-GlcNAc-2-epimerase/ManAc kinase
UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase
NP_446217.1
XP_006238068.1
XP_006238070.1
XP_006238071.1
XP_038965053.1
XP_038965055.1
XP_063143130.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_053765.3 → NP_446217.1  bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase

    See identical proteins and their annotated locations for NP_446217.1

    Status: PROVISIONAL

    Source sequence(s)
    JAXUCZ010000005
    UniProtKB/Swiss-Prot
    O35826
    UniProtKB/TrEMBL
    A0A8I5ZWN3, A6IJ68
    Related
    ENSRNOP00000019532.3, ENSRNOT00000019532.6
    Conserved Domains (2) summary
    COG1940
    Location:409 → 722
    NagC; Sugar kinase of the NBD/HSP70 family, may contain an N-terminal HTH domain [Transcription, Carbohydrate transport and metabolism]
    TIGR03568
    Location:11 → 374
    NeuC_NnaA; UDP-N-acetyl-D-glucosamine 2-epimerase, UDP-hydrolysing

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086023.1 Reference GRCr8

    Range
    63062953..63103320 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006238006.5 → XP_006238068.1  bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X1

    See identical proteins and their annotated locations for XP_006238068.1

    UniProtKB/TrEMBL
    A0A0G2K7T2, A0A8I5ZWN3, A6IJ67
    Related
    ENSRNOP00000074334.1
    Conserved Domains (4) summary
    cd03786
    Location:42 → 406
    GT1_UDP-GlcNAc_2-Epimerase; Bacterial members of the UDP-N-Acetylglucosamine (GlcNAc) 2-Epimerase family are known to catalyze the reversible interconversion of UDP-GlcNAc and UDP-N-acetylmannosamine (UDP-ManNAc). The enzyme serves to produce an activated form of ManNAc residues ...
    COG1940
    Location:440 → 753
    NagC; Sugar kinase of the NBD/HSP70 family, may contain an N-terminal HTH domain [Transcription, Carbohydrate transport and metabolism]
    TIGR03568
    Location:42 → 405
    NeuC_NnaA; UDP-N-acetyl-D-glucosamine 2-epimerase, UDP-hydrolysing
    cl17037
    Location:441 → 719
    NBD_sugar-kinase_HSP70_actin; Nucleotide-Binding Domain of the sugar kinase/HSP70/actin superfamily

  2. XM_006238008.3 → XP_006238070.1  bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X2

    See identical proteins and their annotated locations for XP_006238070.1

    UniProtKB/Swiss-Prot
    O35826
    UniProtKB/TrEMBL
    A0A8I5ZWN3, A6IJ68
    Related
    ENSRNOP00000083164.1
    Conserved Domains (2) summary
    COG1940
    Location:409 → 722
    NagC; Sugar kinase of the NBD/HSP70 family, may contain an N-terminal HTH domain [Transcription, Carbohydrate transport and metabolism]
    TIGR03568
    Location:11 → 374
    NeuC_NnaA; UDP-N-acetyl-D-glucosamine 2-epimerase, UDP-hydrolysing

  3. XM_039109125.2 → XP_038965053.1  bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X2

    UniProtKB/Swiss-Prot
    O35826
    UniProtKB/TrEMBL
    A0A8I5ZWN3, A6IJ68
    Conserved Domains (2) summary
    COG1940
    Location:409 → 722
    NagC; Sugar kinase of the NBD/HSP70 family, may contain an N-terminal HTH domain [Transcription, Carbohydrate transport and metabolism]
    TIGR03568
    Location:11 → 374
    NeuC_NnaA; UDP-N-acetyl-D-glucosamine 2-epimerase, UDP-hydrolysing

  4. XM_006238009.5 → XP_006238071.1  bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X2

    See identical proteins and their annotated locations for XP_006238071.1

    UniProtKB/Swiss-Prot
    O35826
    UniProtKB/TrEMBL
    A0A8I5ZWN3, A6IJ68
    Conserved Domains (2) summary
    COG1940
    Location:409 → 722
    NagC; Sugar kinase of the NBD/HSP70 family, may contain an N-terminal HTH domain [Transcription, Carbohydrate transport and metabolism]
    TIGR03568
    Location:11 → 374
    NeuC_NnaA; UDP-N-acetyl-D-glucosamine 2-epimerase, UDP-hydrolysing

  5. XM_039109127.2 → XP_038965055.1  bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X2

    UniProtKB/Swiss-Prot
    O35826
    UniProtKB/TrEMBL
    A0A8I5ZWN3, A6IJ68
    Conserved Domains (2) summary
    COG1940
    Location:409 → 722
    NagC; Sugar kinase of the NBD/HSP70 family, may contain an N-terminal HTH domain [Transcription, Carbohydrate transport and metabolism]
    TIGR03568
    Location:11 → 374
    NeuC_NnaA; UDP-N-acetyl-D-glucosamine 2-epimerase, UDP-hydrolysing

  6. XM_063287060.1 → XP_063143130.1  bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X3

Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
O35826.1 GenPept UniProtKB/Swiss-Prot:O35826

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